Experiment: Nalidixic 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24510 and PS417_24515 are separated by 89 nucleotides PS417_24515 and PS417_24520 are separated by 224 nucleotides PS417_24520 and PS417_24525 are separated by 25 nucleotides PS417_24525 and PS417_24530 are separated by 117 nucleotides
PS417_24510: PS417_24510 - AsnC family transcriptional regulator, at 5,320,000 to 5,320,461
_24510
PS417_24515: PS417_24515 - acyl carrier protein, at 5,320,551 to 5,320,727
_24515
PS417_24520: PS417_24520 - DNA polymerase III subunit epsilon, at 5,320,952 to 5,321,563
_24520
PS417_24525: PS417_24525 - nuclease, at 5,321,589 to 5,322,065
_24525
PS417_24530: PS417_24530 - ABC transporter substrate-binding protein, at 5,322,183 to 5,322,914
_24530
Position (kb)
5320
5321
5322 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5319.957 kb on - strand at 5320.009 kb on + strand at 5320.009 kb on + strand at 5320.009 kb on + strand at 5320.058 kb on - strand, within PS417_24510 at 5320.216 kb on - strand, within PS417_24510 at 5320.216 kb on - strand, within PS417_24510 at 5320.238 kb on - strand, within PS417_24510 at 5320.326 kb on + strand, within PS417_24510 at 5320.327 kb on - strand, within PS417_24510 at 5320.542 kb on + strand at 5320.542 kb on + strand at 5320.542 kb on + strand at 5320.542 kb on + strand at 5320.542 kb on + strand at 5320.542 kb on + strand at 5320.542 kb on + strand at 5320.543 kb on - strand at 5320.543 kb on - strand at 5320.765 kb on - strand at 5320.821 kb on + strand at 5320.822 kb on - strand at 5320.825 kb on + strand at 5320.826 kb on - strand at 5320.826 kb on - strand at 5320.869 kb on + strand at 5320.869 kb on + strand at 5320.870 kb on - strand at 5320.871 kb on + strand at 5320.871 kb on + strand at 5320.871 kb on + strand at 5320.872 kb on - strand at 5320.872 kb on - strand at 5321.151 kb on + strand, within PS417_24520 at 5321.151 kb on + strand, within PS417_24520 at 5321.265 kb on + strand, within PS417_24520 at 5321.265 kb on + strand, within PS417_24520 at 5321.265 kb on + strand, within PS417_24520 at 5321.266 kb on - strand, within PS417_24520 at 5321.554 kb on + strand at 5321.554 kb on + strand at 5321.554 kb on + strand at 5321.555 kb on - strand at 5321.555 kb on - strand at 5321.555 kb on - strand at 5321.555 kb on - strand at 5321.590 kb on + strand at 5321.595 kb on - strand at 5321.674 kb on + strand, within PS417_24525 at 5321.845 kb on + strand, within PS417_24525 at 5321.846 kb on - strand, within PS417_24525 at 5321.929 kb on + strand, within PS417_24525 at 5321.936 kb on - strand, within PS417_24525 at 5322.022 kb on + strand at 5322.022 kb on + strand at 5322.023 kb on - strand at 5322.026 kb on - strand at 5322.496 kb on + strand, within PS417_24530 at 5322.497 kb on - strand, within PS417_24530 at 5322.497 kb on - strand, within PS417_24530
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.025 mg/ml remove 5,319,957 - -0.9 5,320,009 + +0.9 5,320,009 + -1.8 5,320,009 + -0.2 5,320,058 - PS417_24510 0.13 +0.5 5,320,216 - PS417_24510 0.47 -0.7 5,320,216 - PS417_24510 0.47 +0.1 5,320,238 - PS417_24510 0.52 +0.1 5,320,326 + PS417_24510 0.71 -0.4 5,320,327 - PS417_24510 0.71 -0.4 5,320,542 + +0.2 5,320,542 + -0.3 5,320,542 + -0.9 5,320,542 + +0.7 5,320,542 + -0.0 5,320,542 + +0.4 5,320,542 + -0.3 5,320,543 - +0.3 5,320,543 - +0.3 5,320,765 - -0.5 5,320,821 + -0.0 5,320,822 - +2.1 5,320,825 + +0.2 5,320,826 - +0.4 5,320,826 - +0.2 5,320,869 + -0.1 5,320,869 + -0.2 5,320,870 - -0.1 5,320,871 + +0.2 5,320,871 + +0.8 5,320,871 + +0.2 5,320,872 - +0.5 5,320,872 - +0.2 5,321,151 + PS417_24520 0.33 +0.6 5,321,151 + PS417_24520 0.33 +0.9 5,321,265 + PS417_24520 0.51 +0.2 5,321,265 + PS417_24520 0.51 +1.6 5,321,265 + PS417_24520 0.51 +0.2 5,321,266 - PS417_24520 0.51 +0.7 5,321,554 + -0.1 5,321,554 + +0.6 5,321,554 + -0.5 5,321,555 - -0.5 5,321,555 - -0.1 5,321,555 - -0.2 5,321,555 - -0.8 5,321,590 + +1.4 5,321,595 - -0.1 5,321,674 + PS417_24525 0.18 -0.2 5,321,845 + PS417_24525 0.54 +1.4 5,321,846 - PS417_24525 0.54 -1.4 5,321,929 + PS417_24525 0.71 -0.5 5,321,936 - PS417_24525 0.73 -0.9 5,322,022 + +0.3 5,322,022 + -0.5 5,322,023 - +0.1 5,322,026 - +1.5 5,322,496 + PS417_24530 0.43 -0.2 5,322,497 - PS417_24530 0.43 -0.2 5,322,497 - PS417_24530 0.43 -1.6
Or see this region's nucleotide sequence