Experiment: Nalidixic 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22565 and PS417_22570 are separated by 350 nucleotides PS417_22570 and PS417_22575 are separated by 89 nucleotides PS417_22575 and PS417_22580 are separated by 167 nucleotides PS417_22580 and PS417_22585 overlap by 4 nucleotides
PS417_22565: PS417_22565 - formyltetrahydrofolate deformylase, at 4,886,301 to 4,887,149
_22565
PS417_22570: PS417_22570 - H-NS histone, at 4,887,500 to 4,887,874
_22570
PS417_22575: PS417_22575 - exonuclease I, at 4,887,964 to 4,889,391
_22575
PS417_22580: PS417_22580 - membrane protein, at 4,889,559 to 4,890,251
_22580
PS417_22585: PS417_22585 - membrane protein, at 4,890,248 to 4,891,228
_22585
Position (kb)
4887
4888
4889
4890 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4887.227 kb on + strand at 4887.228 kb on - strand at 4887.258 kb on + strand at 4887.261 kb on + strand at 4887.261 kb on + strand at 4887.283 kb on + strand at 4887.286 kb on - strand at 4887.408 kb on - strand at 4887.411 kb on + strand at 4887.427 kb on + strand at 4887.448 kb on + strand at 4887.449 kb on - strand at 4887.451 kb on + strand at 4887.451 kb on + strand at 4887.483 kb on + strand at 4887.483 kb on + strand at 4887.487 kb on + strand at 4887.488 kb on - strand at 4887.539 kb on - strand, within PS417_22570 at 4887.936 kb on + strand at 4887.937 kb on - strand at 4887.974 kb on - strand at 4887.974 kb on - strand at 4887.998 kb on + strand at 4888.087 kb on - strand at 4888.247 kb on - strand, within PS417_22575 at 4888.283 kb on - strand, within PS417_22575 at 4888.316 kb on + strand, within PS417_22575 at 4888.316 kb on + strand, within PS417_22575 at 4888.435 kb on - strand, within PS417_22575 at 4888.514 kb on + strand, within PS417_22575 at 4888.515 kb on - strand, within PS417_22575 at 4888.571 kb on + strand, within PS417_22575 at 4888.572 kb on - strand, within PS417_22575 at 4888.697 kb on + strand, within PS417_22575 at 4888.728 kb on + strand, within PS417_22575 at 4888.729 kb on - strand, within PS417_22575 at 4888.729 kb on - strand, within PS417_22575 at 4888.729 kb on - strand, within PS417_22575 at 4888.729 kb on - strand, within PS417_22575 at 4888.826 kb on - strand, within PS417_22575 at 4889.072 kb on + strand, within PS417_22575 at 4889.073 kb on - strand, within PS417_22575 at 4889.108 kb on + strand, within PS417_22575 at 4889.299 kb on + strand at 4889.299 kb on + strand at 4889.299 kb on + strand at 4889.366 kb on + strand at 4889.397 kb on - strand at 4889.778 kb on + strand, within PS417_22580 at 4889.778 kb on + strand, within PS417_22580 at 4889.779 kb on - strand, within PS417_22580 at 4889.779 kb on - strand, within PS417_22580 at 4890.042 kb on - strand, within PS417_22580 at 4890.042 kb on - strand, within PS417_22580
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.025 mg/ml remove 4,887,227 + +0.1 4,887,228 - -0.1 4,887,258 + +1.2 4,887,261 + +0.1 4,887,261 + +0.1 4,887,283 + +2.1 4,887,286 - -1.8 4,887,408 - -0.6 4,887,411 + +0.8 4,887,427 + +0.6 4,887,448 + -0.4 4,887,449 - -2.9 4,887,451 + +0.3 4,887,451 + +0.1 4,887,483 + -0.3 4,887,483 + +0.1 4,887,487 + -1.5 4,887,488 - -0.3 4,887,539 - PS417_22570 0.10 -0.1 4,887,936 + -0.6 4,887,937 - +0.9 4,887,974 - -1.2 4,887,974 - +0.2 4,887,998 + -0.7 4,888,087 - +0.1 4,888,247 - PS417_22575 0.20 -1.7 4,888,283 - PS417_22575 0.22 -1.2 4,888,316 + PS417_22575 0.25 -1.4 4,888,316 + PS417_22575 0.25 -1.5 4,888,435 - PS417_22575 0.33 -1.7 4,888,514 + PS417_22575 0.39 -0.5 4,888,515 - PS417_22575 0.39 -3.0 4,888,571 + PS417_22575 0.43 -1.1 4,888,572 - PS417_22575 0.43 -1.5 4,888,697 + PS417_22575 0.51 -1.7 4,888,728 + PS417_22575 0.54 -1.9 4,888,729 - PS417_22575 0.54 -2.4 4,888,729 - PS417_22575 0.54 -0.2 4,888,729 - PS417_22575 0.54 -0.8 4,888,729 - PS417_22575 0.54 -1.7 4,888,826 - PS417_22575 0.60 +0.1 4,889,072 + PS417_22575 0.78 -2.7 4,889,073 - PS417_22575 0.78 -1.7 4,889,108 + PS417_22575 0.80 -1.7 4,889,299 + -1.1 4,889,299 + -4.1 4,889,299 + -1.7 4,889,366 + -2.1 4,889,397 - -1.2 4,889,778 + PS417_22580 0.32 -1.3 4,889,778 + PS417_22580 0.32 +1.3 4,889,779 - PS417_22580 0.32 -0.5 4,889,779 - PS417_22580 0.32 -1.0 4,890,042 - PS417_22580 0.70 -1.8 4,890,042 - PS417_22580 0.70 +0.3
Or see this region's nucleotide sequence