Experiment: Nalidixic 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_20975 and PS417_20980 are separated by 26 nucleotides PS417_20980 and PS417_20985 are separated by 60 nucleotides PS417_20985 and PS417_20990 are separated by 107 nucleotides PS417_20990 and PS417_20995 are separated by 87 nucleotides
PS417_20975: PS417_20975 - protein kinase, at 4,562,939 to 4,564,609
_20975
PS417_20980: PS417_20980 - uroporphyrin-III methyltransferase, at 4,564,636 to 4,565,379
_20980
PS417_20985: PS417_20985 - porin, at 4,565,440 to 4,566,420
_20985
PS417_20990: PS417_20990 - RNA polymerase sigma factor SigX, at 4,566,528 to 4,567,118
_20990
PS417_20995: PS417_20995 - transporter, at 4,567,206 to 4,568,030
_20995
Position (kb)
4565
4566
4567 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4564.589 kb on + strand at 4564.635 kb on + strand at 4564.636 kb on - strand at 4564.859 kb on - strand, within PS417_20980 at 4564.860 kb on + strand, within PS417_20980 at 4564.860 kb on + strand, within PS417_20980 at 4565.134 kb on + strand, within PS417_20980 at 4565.134 kb on + strand, within PS417_20980 at 4565.134 kb on + strand, within PS417_20980 at 4565.135 kb on - strand, within PS417_20980 at 4565.135 kb on - strand, within PS417_20980 at 4565.199 kb on + strand, within PS417_20980 at 4565.268 kb on + strand, within PS417_20980 at 4565.535 kb on - strand at 4565.627 kb on + strand, within PS417_20985 at 4565.677 kb on - strand, within PS417_20985 at 4565.979 kb on - strand, within PS417_20985 at 4566.012 kb on + strand, within PS417_20985 at 4566.446 kb on + strand at 4566.508 kb on + strand at 4566.509 kb on - strand at 4567.088 kb on - strand at 4567.190 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.025 mg/ml remove 4,564,589 + +0.4 4,564,635 + +0.1 4,564,636 - +0.0 4,564,859 - PS417_20980 0.30 +0.0 4,564,860 + PS417_20980 0.30 +0.1 4,564,860 + PS417_20980 0.30 -0.1 4,565,134 + PS417_20980 0.67 -0.8 4,565,134 + PS417_20980 0.67 -0.0 4,565,134 + PS417_20980 0.67 -1.2 4,565,135 - PS417_20980 0.67 -0.2 4,565,135 - PS417_20980 0.67 -0.1 4,565,199 + PS417_20980 0.76 -0.3 4,565,268 + PS417_20980 0.85 +0.2 4,565,535 - -2.2 4,565,627 + PS417_20985 0.19 -2.8 4,565,677 - PS417_20985 0.24 -2.3 4,565,979 - PS417_20985 0.55 -3.1 4,566,012 + PS417_20985 0.58 -1.4 4,566,446 + -1.6 4,566,508 + -1.2 4,566,509 - +0.5 4,567,088 - -0.2 4,567,190 - -1.8
Or see this region's nucleotide sequence