Experiment: Nalidixic 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_20020 and PS417_20025 are separated by 2 nucleotides PS417_20025 and PS417_20030 overlap by 4 nucleotides PS417_20030 and PS417_20035 overlap by 4 nucleotides PS417_20035 and PS417_20040 are separated by 91 nucleotides
PS417_20020: PS417_20020 - cobalamin synthase, at 4,346,019 to 4,346,747
_20020
PS417_20025: PS417_20025 - alpha-ribazole phosphatase, at 4,346,750 to 4,347,325
_20025
PS417_20030: PS417_20030 - nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase, at 4,347,322 to 4,348,377
_20030
PS417_20035: PS417_20035 - adenosylcobinamide kinase, at 4,348,374 to 4,348,895
_20035
PS417_20040: PS417_20040 - cobyric acid synthase, at 4,348,987 to 4,350,438
_20040
Position (kb) 4347
4348
4349 Strain fitness (log2 ratio) -1
0
1 at 4346.864 kb on + strand, within PS417_20025 at 4346.917 kb on - strand, within PS417_20025 at 4347.582 kb on + strand, within PS417_20030 at 4347.583 kb on - strand, within PS417_20030 at 4347.583 kb on - strand, within PS417_20030 at 4347.709 kb on + strand, within PS417_20030 at 4348.807 kb on + strand, within PS417_20035 at 4348.807 kb on + strand, within PS417_20035 at 4348.807 kb on + strand, within PS417_20035 at 4348.807 kb on + strand, within PS417_20035 at 4348.807 kb on + strand, within PS417_20035 at 4348.808 kb on - strand, within PS417_20035 at 4348.808 kb on - strand, within PS417_20035 at 4348.808 kb on - strand, within PS417_20035 at 4348.808 kb on - strand, within PS417_20035 at 4348.978 kb on + strand at 4349.165 kb on + strand, within PS417_20040 at 4349.165 kb on + strand, within PS417_20040 at 4349.166 kb on - strand, within PS417_20040
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.025 mg/ml remove 4,346,864 + PS417_20025 0.20 -0.5 4,346,917 - PS417_20025 0.29 -0.4 4,347,582 + PS417_20030 0.25 -1.4 4,347,583 - PS417_20030 0.25 +1.0 4,347,583 - PS417_20030 0.25 -0.3 4,347,709 + PS417_20030 0.37 -0.6 4,348,807 + PS417_20035 0.83 -1.1 4,348,807 + PS417_20035 0.83 -0.0 4,348,807 + PS417_20035 0.83 -0.7 4,348,807 + PS417_20035 0.83 +0.1 4,348,807 + PS417_20035 0.83 +1.0 4,348,808 - PS417_20035 0.83 -0.0 4,348,808 - PS417_20035 0.83 -0.5 4,348,808 - PS417_20035 0.83 -0.1 4,348,808 - PS417_20035 0.83 +0.6 4,348,978 + +1.0 4,349,165 + PS417_20040 0.12 -0.2 4,349,165 + PS417_20040 0.12 +0.0 4,349,166 - PS417_20040 0.12 +0.0
Or see this region's nucleotide sequence