Experiment: Nalidixic 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19865 and PS417_19870 are separated by 10 nucleotides PS417_19870 and PS417_19875 are separated by 4 nucleotides
PS417_19865: PS417_19865 - flagellar hook protein FlgK, at 4,313,343 to 4,315,388
_19865
PS417_19870: PS417_19870 - flagellar rod assembly protein FlgJ, at 4,315,399 to 4,316,670
_19870
PS417_19875: PS417_19875 - flagellar P-ring protein FlgI, at 4,316,675 to 4,317,781
_19875
Position (kb)
4315
4316
4317 Strain fitness (log2 ratio)
-1
0
1
2
3 at 4314.564 kb on - strand at 4314.579 kb on + strand, within PS417_19865 at 4314.579 kb on + strand, within PS417_19865 at 4314.631 kb on + strand, within PS417_19865 at 4314.727 kb on - strand, within PS417_19865 at 4314.727 kb on - strand, within PS417_19865 at 4314.777 kb on + strand, within PS417_19865 at 4314.778 kb on - strand, within PS417_19865 at 4314.789 kb on - strand, within PS417_19865 at 4314.789 kb on - strand, within PS417_19865 at 4315.129 kb on + strand, within PS417_19865 at 4315.129 kb on + strand, within PS417_19865 at 4315.130 kb on - strand, within PS417_19865 at 4315.192 kb on + strand at 4315.357 kb on - strand at 4315.401 kb on - strand at 4315.401 kb on - strand at 4315.402 kb on + strand at 4315.402 kb on + strand at 4315.402 kb on + strand at 4315.402 kb on + strand at 4315.402 kb on + strand at 4315.402 kb on + strand at 4315.403 kb on - strand at 4315.403 kb on - strand at 4315.403 kb on - strand at 4315.431 kb on - strand at 4315.625 kb on + strand, within PS417_19870 at 4315.626 kb on - strand, within PS417_19870 at 4316.230 kb on + strand, within PS417_19870 at 4316.420 kb on + strand, within PS417_19870 at 4316.420 kb on + strand at 4316.432 kb on + strand, within PS417_19870 at 4316.616 kb on - strand at 4316.616 kb on - strand at 4317.278 kb on - strand, within PS417_19875
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.025 mg/ml remove 4,314,564 - +0.6 4,314,579 + PS417_19865 0.60 +1.8 4,314,579 + PS417_19865 0.60 +2.0 4,314,631 + PS417_19865 0.63 +2.2 4,314,727 - PS417_19865 0.68 +0.5 4,314,727 - PS417_19865 0.68 +2.2 4,314,777 + PS417_19865 0.70 +2.4 4,314,778 - PS417_19865 0.70 +3.0 4,314,789 - PS417_19865 0.71 +1.5 4,314,789 - PS417_19865 0.71 +2.5 4,315,129 + PS417_19865 0.87 +2.7 4,315,129 + PS417_19865 0.87 +1.2 4,315,130 - PS417_19865 0.87 +1.4 4,315,192 + +2.0 4,315,357 - +2.6 4,315,401 - -0.1 4,315,401 - -0.0 4,315,402 + +1.8 4,315,402 + +1.9 4,315,402 + +3.5 4,315,402 + +3.7 4,315,402 + +2.1 4,315,402 + +1.9 4,315,403 - -0.4 4,315,403 - -0.1 4,315,403 - -1.0 4,315,431 - -0.0 4,315,625 + PS417_19870 0.18 +1.5 4,315,626 - PS417_19870 0.18 +0.4 4,316,230 + PS417_19870 0.65 -0.2 4,316,420 + PS417_19870 0.80 +2.3 4,316,420 + +3.3 4,316,432 + PS417_19870 0.81 +1.7 4,316,616 - -0.1 4,316,616 - -0.2 4,317,278 - PS417_19875 0.54 +2.9
Or see this region's nucleotide sequence