Experiment: Nalidixic 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19800 and PS417_19805 overlap by 8 nucleotides PS417_19805 and PS417_19810 are separated by 16 nucleotides PS417_19810 and PS417_19815 are separated by 131 nucleotides
PS417_19800: PS417_19800 - flagellar motor switch protein FliG, at 4,298,448 to 4,299,467
_19800
PS417_19805: PS417_19805 - flagellar M-ring protein FliF, at 4,299,460 to 4,301,238
_19805
PS417_19810: PS417_19810 - flagellar hook-basal body protein FliE, at 4,301,255 to 4,301,584
_19810
PS417_19815: PS417_19815 - Fis family transcriptional regulator, at 4,301,716 to 4,303,092
_19815
Position (kb)
4299
4300
4301
4302 Strain fitness (log2 ratio)
-1
0
1
2 at 4299.220 kb on + strand at 4299.221 kb on - strand, within PS417_19800 at 4299.221 kb on - strand, within PS417_19800 at 4299.555 kb on - strand at 4299.800 kb on + strand, within PS417_19805 at 4300.702 kb on + strand, within PS417_19805 at 4301.244 kb on - strand at 4301.283 kb on + strand at 4301.303 kb on - strand, within PS417_19810 at 4301.303 kb on - strand, within PS417_19810 at 4301.634 kb on + strand at 4301.634 kb on + strand at 4301.635 kb on - strand at 4301.645 kb on + strand at 4301.718 kb on - strand at 4301.718 kb on - strand at 4301.728 kb on + strand at 4301.729 kb on - strand at 4301.729 kb on - strand at 4301.732 kb on + strand at 4301.732 kb on + strand at 4301.733 kb on - strand at 4301.733 kb on - strand at 4301.733 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.025 mg/ml remove 4,299,220 + -0.0 4,299,221 - PS417_19800 0.76 +0.8 4,299,221 - PS417_19800 0.76 +1.0 4,299,555 - +0.8 4,299,800 + PS417_19805 0.19 +0.9 4,300,702 + PS417_19805 0.70 +2.6 4,301,244 - +1.4 4,301,283 + +1.0 4,301,303 - PS417_19810 0.15 +2.4 4,301,303 - PS417_19810 0.15 +2.4 4,301,634 + +1.0 4,301,634 + +0.9 4,301,635 - +0.8 4,301,645 + +0.8 4,301,718 - +1.6 4,301,718 - -1.1 4,301,728 + +1.5 4,301,729 - -0.2 4,301,729 - +0.3 4,301,732 + +1.6 4,301,732 + +1.6 4,301,733 - -1.6 4,301,733 - -0.1 4,301,733 - +0.8
Or see this region's nucleotide sequence