Experiment: Nalidixic 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18035 and PS417_18040 are separated by 5 nucleotides PS417_18040 and PS417_18045 overlap by 4 nucleotides PS417_18045 and PS417_18050 overlap by 26 nucleotides
PS417_18035: PS417_18035 - general secretion pathway protein GspD, at 3,930,311 to 3,932,422
_18035
PS417_18040: PS417_18040 - general secretion pathway protein GspN, at 3,932,428 to 3,932,946
_18040
PS417_18045: PS417_18045 - general secretion pathway protein GspM, at 3,932,943 to 3,933,545
_18045
PS417_18050: PS417_18050 - general secretion pathway protein GspL, at 3,933,520 to 3,934,512
_18050
Position (kb)
3932
3933 Strain fitness (log2 ratio)
-1
0
1
2 at 3931.443 kb on + strand, within PS417_18035 at 3931.443 kb on + strand at 3931.444 kb on - strand, within PS417_18035 at 3931.444 kb on - strand, within PS417_18035 at 3931.444 kb on - strand, within PS417_18035 at 3931.720 kb on - strand, within PS417_18035 at 3931.720 kb on - strand, within PS417_18035 at 3931.843 kb on - strand, within PS417_18035 at 3931.947 kb on + strand, within PS417_18035 at 3932.083 kb on - strand, within PS417_18035 at 3932.454 kb on + strand at 3932.894 kb on - strand, within PS417_18040 at 3933.382 kb on + strand, within PS417_18045 at 3933.562 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.025 mg/ml remove 3,931,443 + PS417_18035 0.54 +2.0 3,931,443 + +2.5 3,931,444 - PS417_18035 0.54 -0.4 3,931,444 - PS417_18035 0.54 -0.3 3,931,444 - PS417_18035 0.54 -0.9 3,931,720 - PS417_18035 0.67 -0.3 3,931,720 - PS417_18035 0.67 +0.7 3,931,843 - PS417_18035 0.73 -0.1 3,931,947 + PS417_18035 0.77 +2.4 3,932,083 - PS417_18035 0.84 +0.1 3,932,454 + -0.1 3,932,894 - PS417_18040 0.90 -0.7 3,933,382 + PS417_18045 0.73 -0.5 3,933,562 + -0.7
Or see this region's nucleotide sequence