Experiment: Nalidixic 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_17830 and PS417_17835 overlap by 4 nucleotides PS417_17835 and PS417_17840 are separated by 5 nucleotides PS417_17840 and PS417_17845 are separated by 49 nucleotides PS417_17845 and PS417_17850 are separated by 15 nucleotides
PS417_17830: PS417_17830 - 4-hydroxyphenylacetate isomerase, at 3,893,873 to 3,894,538
_17830
PS417_17835: PS417_17835 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 3,894,535 to 3,895,305
_17835
PS417_17840: PS417_17840 - betaine-aldehyde dehydrogenase, at 3,895,311 to 3,896,771
_17840
PS417_17845: PS417_17845 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 3,896,821 to 3,897,678
_17845
PS417_17850: PS417_17850 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 3,897,694 to 3,898,107
_17850
Position (kb)
3895
3896
3897 Strain fitness (log2 ratio)
-1
0
1
2 at 3894.515 kb on - strand at 3894.766 kb on + strand, within PS417_17835 at 3894.766 kb on + strand, within PS417_17835 at 3894.767 kb on - strand, within PS417_17835 at 3894.821 kb on - strand, within PS417_17835 at 3894.821 kb on - strand, within PS417_17835 at 3894.837 kb on - strand, within PS417_17835 at 3895.130 kb on - strand at 3895.368 kb on + strand at 3895.369 kb on - strand at 3895.369 kb on - strand at 3895.369 kb on - strand at 3895.369 kb on - strand at 3895.698 kb on + strand, within PS417_17840 at 3895.699 kb on - strand, within PS417_17840 at 3896.542 kb on - strand, within PS417_17840 at 3896.792 kb on - strand at 3896.805 kb on - strand at 3896.858 kb on + strand at 3896.870 kb on - strand at 3897.046 kb on + strand, within PS417_17845 at 3897.088 kb on + strand, within PS417_17845 at 3897.217 kb on + strand, within PS417_17845 at 3897.217 kb on + strand, within PS417_17845 at 3897.218 kb on - strand, within PS417_17845
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.025 mg/ml remove 3,894,515 - +0.3 3,894,766 + PS417_17835 0.30 -0.9 3,894,766 + PS417_17835 0.30 +1.1 3,894,767 - PS417_17835 0.30 -0.3 3,894,821 - PS417_17835 0.37 +0.6 3,894,821 - PS417_17835 0.37 +0.6 3,894,837 - PS417_17835 0.39 -0.1 3,895,130 - +0.9 3,895,368 + +0.9 3,895,369 - +1.9 3,895,369 - -0.6 3,895,369 - +0.5 3,895,369 - -0.5 3,895,698 + PS417_17840 0.26 +1.2 3,895,699 - PS417_17840 0.27 +0.9 3,896,542 - PS417_17840 0.84 +0.6 3,896,792 - +0.5 3,896,805 - +0.3 3,896,858 + -0.4 3,896,870 - -0.7 3,897,046 + PS417_17845 0.26 -0.2 3,897,088 + PS417_17845 0.31 +0.1 3,897,217 + PS417_17845 0.46 -0.7 3,897,217 + PS417_17845 0.46 -0.1 3,897,218 - PS417_17845 0.46 +0.4
Or see this region's nucleotide sequence