Experiment: Nalidixic 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13110 and PS417_13115 are separated by 141 nucleotides PS417_13115 and PS417_13120 are separated by 297 nucleotides PS417_13120 and PS417_13125 are separated by 21 nucleotides
PS417_13110: PS417_13110 - methylase, at 2,864,997 to 2,865,809
_13110
PS417_13115: PS417_13115 - hypothetical protein, at 2,865,951 to 2,866,337
_13115
PS417_13120: PS417_13120 - aspartyl beta-hydroxylase, at 2,866,635 to 2,867,534
_13120
PS417_13125: PS417_13125 - ATP-dependent DNA ligase, at 2,867,556 to 2,870,015
_13125
Position (kb)
2866
2867
2868 Strain fitness (log2 ratio)
-2
-1
0
1 at 2865.636 kb on + strand, within PS417_13110 at 2865.636 kb on + strand, within PS417_13110 at 2865.636 kb on + strand, within PS417_13110 at 2865.735 kb on + strand at 2865.780 kb on - strand at 2865.879 kb on + strand at 2865.879 kb on + strand at 2865.891 kb on + strand at 2865.891 kb on + strand at 2865.892 kb on - strand at 2865.893 kb on + strand at 2866.086 kb on - strand, within PS417_13115 at 2866.118 kb on + strand, within PS417_13115 at 2866.324 kb on - strand at 2866.338 kb on - strand at 2866.338 kb on - strand at 2866.338 kb on - strand at 2866.338 kb on - strand at 2866.460 kb on + strand at 2866.460 kb on + strand at 2866.678 kb on - strand at 2866.678 kb on - strand at 2866.678 kb on - strand at 2866.678 kb on - strand at 2866.678 kb on - strand at 2866.678 kb on - strand at 2866.681 kb on + strand at 2866.681 kb on + strand at 2866.682 kb on - strand at 2866.682 kb on - strand at 2866.768 kb on - strand, within PS417_13120 at 2866.797 kb on + strand, within PS417_13120 at 2866.797 kb on + strand, within PS417_13120 at 2866.911 kb on + strand, within PS417_13120 at 2866.963 kb on - strand, within PS417_13120 at 2867.121 kb on + strand, within PS417_13120 at 2867.190 kb on + strand, within PS417_13120 at 2867.191 kb on - strand, within PS417_13120 at 2867.191 kb on - strand, within PS417_13120 at 2867.232 kb on + strand, within PS417_13120 at 2867.233 kb on - strand, within PS417_13120 at 2867.424 kb on + strand, within PS417_13120 at 2867.621 kb on - strand at 2867.803 kb on + strand, within PS417_13125 at 2868.365 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.025 mg/ml remove 2,865,636 + PS417_13110 0.79 -0.1 2,865,636 + PS417_13110 0.79 -0.1 2,865,636 + PS417_13110 0.79 +0.0 2,865,735 + +0.2 2,865,780 - -0.1 2,865,879 + +1.4 2,865,879 + -0.3 2,865,891 + +0.2 2,865,891 + -1.1 2,865,892 - +0.8 2,865,893 + +0.0 2,866,086 - PS417_13115 0.35 +0.5 2,866,118 + PS417_13115 0.43 -0.1 2,866,324 - -0.7 2,866,338 - +0.2 2,866,338 - +0.1 2,866,338 - -0.5 2,866,338 - -0.8 2,866,460 + -0.5 2,866,460 + +0.5 2,866,678 - +0.8 2,866,678 - -0.5 2,866,678 - -0.5 2,866,678 - -0.3 2,866,678 - -1.1 2,866,678 - +0.2 2,866,681 + +0.2 2,866,681 + -0.4 2,866,682 - -2.0 2,866,682 - -0.5 2,866,768 - PS417_13120 0.15 -0.5 2,866,797 + PS417_13120 0.18 +0.2 2,866,797 + PS417_13120 0.18 -0.7 2,866,911 + PS417_13120 0.31 -1.7 2,866,963 - PS417_13120 0.36 -1.5 2,867,121 + PS417_13120 0.54 -0.8 2,867,190 + PS417_13120 0.62 -0.8 2,867,191 - PS417_13120 0.62 -1.4 2,867,191 - PS417_13120 0.62 -0.2 2,867,232 + PS417_13120 0.66 +0.8 2,867,233 - PS417_13120 0.66 -1.5 2,867,424 + PS417_13120 0.88 -0.7 2,867,621 - -0.8 2,867,803 + PS417_13125 0.10 +0.6 2,868,365 - -0.2
Or see this region's nucleotide sequence