Strain Fitness in Pseudomonas simiae WCS417 around PS417_12235

Experiment: Nalidixic 0.025 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_12225 and PS417_12230 are separated by 30 nucleotidesPS417_12230 and PS417_12235 are separated by 64 nucleotidesPS417_12235 and PS417_12240 are separated by 127 nucleotides PS417_12225: PS417_12225 - SAM-dependent methyltransferase, at 2,657,747 to 2,658,505 _12225 PS417_12230: PS417_12230 - ribonuclease H, at 2,658,536 to 2,658,988 _12230 PS417_12235: PS417_12235 - DNA polymerase III subunit epsilon, at 2,659,053 to 2,659,799 _12235 PS417_12240: PS417_12240 - lysine decarboxylase, at 2,659,927 to 2,662,182 _12240 Position (kb) 2659 2660Strain fitness (log2 ratio) -2 -1 0 1at 2659.004 kb on + strandat 2659.007 kb on + strandat 2659.833 kb on + strandat 2659.834 kb on - strandat 2659.889 kb on + strandat 2659.916 kb on + strandat 2660.142 kb on + strandat 2660.143 kb on - strandat 2660.233 kb on - strand, within PS417_12240at 2660.354 kb on - strand, within PS417_12240at 2660.354 kb on - strand, within PS417_12240at 2660.416 kb on + strand, within PS417_12240at 2660.416 kb on + strand, within PS417_12240at 2660.764 kb on + strand, within PS417_12240at 2660.764 kb on + strand, within PS417_12240at 2660.765 kb on - strand, within PS417_12240at 2660.765 kb on - strand, within PS417_12240at 2660.765 kb on - strand, within PS417_12240

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Per-strain Table

Position Strand Gene LocusTag Fraction Nalidixic 0.025 mg/ml
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2,659,004 + -0.9
2,659,007 + +0.4
2,659,833 + -0.4
2,659,834 - +0.8
2,659,889 + -0.3
2,659,916 + +0.3
2,660,142 + +0.1
2,660,143 - +0.8
2,660,233 - PS417_12240 0.14 -0.5
2,660,354 - PS417_12240 0.19 -2.1
2,660,354 - PS417_12240 0.19 +0.5
2,660,416 + PS417_12240 0.22 +0.8
2,660,416 + PS417_12240 0.22 -0.5
2,660,764 + PS417_12240 0.37 -0.2
2,660,764 + PS417_12240 0.37 -0.4
2,660,765 - PS417_12240 0.37 +0.2
2,660,765 - PS417_12240 0.37 +1.4
2,660,765 - PS417_12240 0.37 -0.2

Or see this region's nucleotide sequence