Experiment: Nalidixic 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05650 and PS417_05655 are separated by 107 nucleotides PS417_05655 and PS417_05660 are separated by 65 nucleotides PS417_05660 and PS417_05665 are separated by 214 nucleotides
PS417_05650: PS417_05650 - leucyl-tRNA synthetase, at 1,270,380 to 1,270,562
_05650
PS417_05655: PS417_05655 - endonuclease III, at 1,270,670 to 1,271,308
_05655
PS417_05660: PS417_05660 - (4Fe-4S)-binding protein, at 1,271,374 to 1,272,327
_05660
PS417_05665: PS417_05665 - methionine--tRNA ligase, at 1,272,542 to 1,274,593
_05665
Position (kb)
1271
1272
1273 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1270.445 kb on + strand, within PS417_05650 at 1270.445 kb on + strand, within PS417_05650 at 1270.500 kb on + strand, within PS417_05650 at 1270.500 kb on + strand, within PS417_05650 at 1270.501 kb on - strand at 1270.501 kb on - strand, within PS417_05650 at 1270.562 kb on + strand at 1270.562 kb on + strand at 1270.562 kb on + strand at 1270.563 kb on - strand at 1270.580 kb on - strand at 1270.601 kb on + strand at 1270.602 kb on - strand at 1270.604 kb on - strand at 1270.604 kb on - strand at 1270.755 kb on + strand, within PS417_05655 at 1270.755 kb on + strand, within PS417_05655 at 1270.756 kb on - strand at 1270.889 kb on - strand, within PS417_05655 at 1270.889 kb on - strand, within PS417_05655 at 1271.065 kb on - strand, within PS417_05655 at 1271.086 kb on - strand, within PS417_05655 at 1271.115 kb on + strand, within PS417_05655 at 1271.115 kb on + strand, within PS417_05655 at 1271.115 kb on + strand, within PS417_05655 at 1271.115 kb on + strand at 1271.347 kb on - strand at 1271.347 kb on - strand at 1271.416 kb on + strand at 1271.416 kb on + strand at 1271.417 kb on - strand at 1271.417 kb on - strand at 1271.529 kb on + strand at 1271.676 kb on + strand, within PS417_05660 at 1271.676 kb on + strand, within PS417_05660 at 1271.677 kb on - strand, within PS417_05660 at 1271.677 kb on - strand, within PS417_05660 at 1271.742 kb on + strand, within PS417_05660 at 1271.759 kb on - strand, within PS417_05660 at 1271.835 kb on + strand, within PS417_05660 at 1271.835 kb on + strand, within PS417_05660 at 1271.836 kb on - strand, within PS417_05660 at 1271.836 kb on - strand, within PS417_05660 at 1271.836 kb on - strand, within PS417_05660 at 1271.926 kb on + strand, within PS417_05660 at 1271.927 kb on - strand, within PS417_05660 at 1272.105 kb on - strand, within PS417_05660 at 1272.456 kb on - strand at 1272.526 kb on + strand at 1272.527 kb on - strand at 1272.543 kb on + strand at 1272.543 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.025 mg/ml remove 1,270,445 + PS417_05650 0.36 +0.7 1,270,445 + PS417_05650 0.36 +0.7 1,270,500 + PS417_05650 0.66 -1.8 1,270,500 + PS417_05650 0.66 +0.3 1,270,501 - +0.0 1,270,501 - PS417_05650 0.66 +0.4 1,270,562 + +0.2 1,270,562 + -0.7 1,270,562 + +0.4 1,270,563 - +0.3 1,270,580 - +1.4 1,270,601 + +0.4 1,270,602 - -0.3 1,270,604 - +0.9 1,270,604 - +0.3 1,270,755 + PS417_05655 0.13 -0.3 1,270,755 + PS417_05655 0.13 +0.6 1,270,756 - -0.2 1,270,889 - PS417_05655 0.34 +1.2 1,270,889 - PS417_05655 0.34 -1.0 1,271,065 - PS417_05655 0.62 +0.2 1,271,086 - PS417_05655 0.65 -0.2 1,271,115 + PS417_05655 0.70 -0.6 1,271,115 + PS417_05655 0.70 +0.0 1,271,115 + PS417_05655 0.70 +0.0 1,271,115 + -0.4 1,271,347 - -0.2 1,271,347 - -0.1 1,271,416 + -0.5 1,271,416 + -0.3 1,271,417 - -1.0 1,271,417 - -0.3 1,271,529 + +1.0 1,271,676 + PS417_05660 0.32 +0.4 1,271,676 + PS417_05660 0.32 -0.5 1,271,677 - PS417_05660 0.32 +1.2 1,271,677 - PS417_05660 0.32 +1.0 1,271,742 + PS417_05660 0.39 +0.4 1,271,759 - PS417_05660 0.40 +1.5 1,271,835 + PS417_05660 0.48 +0.4 1,271,835 + PS417_05660 0.48 +1.3 1,271,836 - PS417_05660 0.48 -0.0 1,271,836 - PS417_05660 0.48 +0.4 1,271,836 - PS417_05660 0.48 +2.3 1,271,926 + PS417_05660 0.58 +0.6 1,271,927 - PS417_05660 0.58 +0.2 1,272,105 - PS417_05660 0.77 +1.0 1,272,456 - -0.8 1,272,526 + +0.3 1,272,527 - -0.2 1,272,543 + +0.6 1,272,543 + -1.0
Or see this region's nucleotide sequence