Experiment: m.b. Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA264_20055 and CA264_20060 are separated by 69 nucleotides CA264_20060 and CA264_20065 are separated by 50 nucleotides CA264_20065 and CA264_20070 overlap by 4 nucleotides
CA264_20055: CA264_20055 - peptidase M41, at 4,621,910 to 4,624,033
_20055
CA264_20060: CA264_20060 - ribosome silencing factor RsfS, at 4,624,103 to 4,624,477
_20060
CA264_20065: CA264_20065 - biotin--[acetyl-CoA-carboxylase] ligase, at 4,624,528 to 4,625,277
_20065
CA264_20070: CA264_20070 - hypothetical protein, at 4,625,274 to 4,625,846
_20070
Position (kb)
4624
4625 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4623.800 kb on + strand, within CA264_20055 at 4623.902 kb on + strand at 4624.042 kb on - strand at 4624.042 kb on - strand at 4624.124 kb on + strand at 4624.124 kb on + strand at 4624.193 kb on + strand, within CA264_20060 at 4624.194 kb on - strand, within CA264_20060 at 4624.197 kb on - strand, within CA264_20060 at 4624.340 kb on + strand, within CA264_20060 at 4624.499 kb on + strand at 4624.500 kb on - strand at 4624.521 kb on + strand at 4624.524 kb on + strand at 4624.525 kb on - strand at 4624.525 kb on - strand at 4624.527 kb on + strand at 4624.527 kb on + strand at 4624.527 kb on + strand at 4624.528 kb on - strand at 4625.272 kb on - strand at 4625.385 kb on + strand, within CA264_20070
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Dimethyl Sulfoxide 10 vol% remove 4,623,800 + CA264_20055 0.89 -3.4 4,623,902 + -0.2 4,624,042 - -0.5 4,624,042 - +0.6 4,624,124 + -0.9 4,624,124 + -0.5 4,624,193 + CA264_20060 0.24 -1.8 4,624,194 - CA264_20060 0.24 +0.2 4,624,197 - CA264_20060 0.25 -2.2 4,624,340 + CA264_20060 0.63 -0.9 4,624,499 + -0.7 4,624,500 - -2.3 4,624,521 + -0.3 4,624,524 + -0.9 4,624,525 - +0.3 4,624,525 - -1.8 4,624,527 + +0.2 4,624,527 + -0.1 4,624,527 + -0.7 4,624,528 - -0.7 4,625,272 - -2.2 4,625,385 + CA264_20070 0.19 -1.0
Or see this region's nucleotide sequence