Experiment: Nalidixic 0.015 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_25640 and PS417_25645 are separated by 209 nucleotides PS417_25645 and PS417_25650 are separated by 3 nucleotides
PS417_25640: PS417_25640 - tyrosine--tRNA ligase, at 5,538,382 to 5,539,581
_25640
PS417_25645: PS417_25645 - peptidase M23, at 5,539,791 to 5,541,209
_25645
PS417_25650: PS417_25650 - anhydro-N-acetylmuramic acid kinase, at 5,541,213 to 5,542,304
_25650
Position (kb)
5539
5540
5541
5542 Strain fitness (log2 ratio)
-1
0
1
2 at 5539.623 kb on - strand at 5539.623 kb on - strand at 5539.638 kb on - strand at 5539.718 kb on + strand at 5539.786 kb on + strand at 5539.786 kb on + strand at 5540.415 kb on - strand, within PS417_25645 at 5540.641 kb on - strand, within PS417_25645 at 5540.667 kb on + strand, within PS417_25645 at 5540.670 kb on + strand, within PS417_25645 at 5540.687 kb on + strand, within PS417_25645 at 5540.687 kb on + strand, within PS417_25645 at 5540.732 kb on + strand, within PS417_25645 at 5540.732 kb on + strand, within PS417_25645 at 5540.733 kb on - strand, within PS417_25645 at 5540.916 kb on + strand, within PS417_25645 at 5541.042 kb on + strand, within PS417_25645 at 5541.222 kb on + strand at 5541.222 kb on + strand at 5541.312 kb on + strand at 5541.313 kb on - strand at 5541.313 kb on - strand at 5541.329 kb on + strand, within PS417_25650 at 5541.330 kb on - strand, within PS417_25650 at 5541.334 kb on + strand, within PS417_25650 at 5541.576 kb on - strand, within PS417_25650 at 5541.576 kb on - strand, within PS417_25650 at 5541.803 kb on - strand, within PS417_25650
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.015 mg/ml remove 5,539,623 - +0.5 5,539,623 - +0.2 5,539,638 - +2.1 5,539,718 + -0.9 5,539,786 + +1.5 5,539,786 + +0.6 5,540,415 - PS417_25645 0.44 +0.3 5,540,641 - PS417_25645 0.60 +1.1 5,540,667 + PS417_25645 0.62 -0.1 5,540,670 + PS417_25645 0.62 +0.5 5,540,687 + PS417_25645 0.63 +1.1 5,540,687 + PS417_25645 0.63 +0.3 5,540,732 + PS417_25645 0.66 +1.9 5,540,732 + PS417_25645 0.66 +0.8 5,540,733 - PS417_25645 0.66 +0.1 5,540,916 + PS417_25645 0.79 +1.3 5,541,042 + PS417_25645 0.88 +0.5 5,541,222 + +0.3 5,541,222 + +0.8 5,541,312 + -0.1 5,541,313 - -0.2 5,541,313 - +1.3 5,541,329 + PS417_25650 0.11 +1.3 5,541,330 - PS417_25650 0.11 +1.0 5,541,334 + PS417_25650 0.11 +1.1 5,541,576 - PS417_25650 0.33 +0.2 5,541,576 - PS417_25650 0.33 -0.5 5,541,803 - PS417_25650 0.54 +0.8
Or see this region's nucleotide sequence