Experiment: Nalidixic 0.015 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18345 and PS417_18350 are separated by 367 nucleotides PS417_18350 and PS417_18355 are separated by 289 nucleotides PS417_18355 and PS417_18360 are separated by 73 nucleotides
PS417_18345: PS417_18345 - threonyl-tRNA synthetase, at 3,996,350 to 3,998,272
_18345
PS417_18350: PS417_18350 - hypothetical protein, at 3,998,640 to 3,998,954
_18350
PS417_18355: PS417_18355 - DNA-binding protein, at 3,999,244 to 3,999,456
_18355
PS417_18360: PS417_18360 - hypothetical protein, at 3,999,530 to 3,999,847
_18360
Position (kb)
3998
3999 Strain fitness (log2 ratio)
-2
-1
0
1 at 3998.523 kb on + strand at 3998.523 kb on + strand at 3998.524 kb on - strand at 3998.533 kb on - strand at 3998.552 kb on + strand at 3998.553 kb on - strand at 3998.553 kb on - strand at 3998.554 kb on + strand at 3998.554 kb on + strand at 3998.554 kb on + strand at 3998.555 kb on - strand at 3998.555 kb on - strand at 3998.555 kb on - strand at 3998.555 kb on - strand at 3998.579 kb on + strand at 3998.579 kb on + strand at 3998.651 kb on + strand at 3998.658 kb on - strand at 3998.785 kb on + strand, within PS417_18350 at 3998.785 kb on + strand, within PS417_18350 at 3998.786 kb on - strand, within PS417_18350 at 3999.023 kb on - strand at 3999.046 kb on - strand at 3999.056 kb on - strand at 3999.117 kb on + strand at 3999.118 kb on - strand at 3999.170 kb on - strand at 3999.175 kb on - strand at 3999.175 kb on - strand at 3999.221 kb on + strand at 3999.221 kb on + strand at 3999.221 kb on + strand at 3999.222 kb on - strand at 3999.222 kb on - strand at 3999.223 kb on + strand at 3999.223 kb on + strand at 3999.224 kb on - strand at 3999.224 kb on - strand at 3999.238 kb on + strand at 3999.238 kb on + strand at 3999.238 kb on + strand at 3999.238 kb on + strand at 3999.238 kb on + strand at 3999.239 kb on - strand at 3999.239 kb on - strand at 3999.249 kb on + strand at 3999.250 kb on - strand at 3999.271 kb on - strand, within PS417_18355 at 3999.271 kb on - strand, within PS417_18355 at 3999.338 kb on + strand, within PS417_18355 at 3999.338 kb on + strand, within PS417_18355 at 3999.338 kb on + strand, within PS417_18355 at 3999.339 kb on - strand, within PS417_18355 at 3999.339 kb on - strand, within PS417_18355 at 3999.339 kb on - strand, within PS417_18355 at 3999.454 kb on + strand at 3999.454 kb on + strand at 3999.470 kb on + strand at 3999.562 kb on + strand, within PS417_18360 at 3999.597 kb on + strand, within PS417_18360 at 3999.598 kb on - strand, within PS417_18360 at 3999.614 kb on - strand, within PS417_18360 at 3999.857 kb on + strand at 3999.857 kb on + strand at 3999.857 kb on + strand at 3999.857 kb on + strand at 3999.858 kb on - strand at 3999.916 kb on + strand at 3999.917 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.015 mg/ml remove 3,998,523 + -0.8 3,998,523 + -0.4 3,998,524 - -0.3 3,998,533 - -0.4 3,998,552 + -0.4 3,998,553 - -0.6 3,998,553 - +1.2 3,998,554 + +0.9 3,998,554 + +0.5 3,998,554 + -0.2 3,998,555 - -0.6 3,998,555 - +0.2 3,998,555 - -0.5 3,998,555 - -0.4 3,998,579 + -0.3 3,998,579 + -0.3 3,998,651 + +1.2 3,998,658 - -0.2 3,998,785 + PS417_18350 0.46 -0.7 3,998,785 + PS417_18350 0.46 -0.8 3,998,786 - PS417_18350 0.46 -1.3 3,999,023 - -2.2 3,999,046 - -1.5 3,999,056 - -1.0 3,999,117 + -0.5 3,999,118 - -0.3 3,999,170 - -1.4 3,999,175 - -0.9 3,999,175 - -0.0 3,999,221 + -1.6 3,999,221 + -1.1 3,999,221 + -1.0 3,999,222 - -1.7 3,999,222 - -1.0 3,999,223 + -1.6 3,999,223 + -2.6 3,999,224 - -2.0 3,999,224 - -0.9 3,999,238 + -1.5 3,999,238 + -1.0 3,999,238 + -1.6 3,999,238 + -1.9 3,999,238 + -1.0 3,999,239 - -1.4 3,999,239 - -1.6 3,999,249 + -1.2 3,999,250 - -2.0 3,999,271 - PS417_18355 0.13 -0.5 3,999,271 - PS417_18355 0.13 -1.4 3,999,338 + PS417_18355 0.44 -1.2 3,999,338 + PS417_18355 0.44 -1.7 3,999,338 + PS417_18355 0.44 -1.5 3,999,339 - PS417_18355 0.45 -2.3 3,999,339 - PS417_18355 0.45 -1.5 3,999,339 - PS417_18355 0.45 -0.9 3,999,454 + -0.6 3,999,454 + -2.2 3,999,470 + -1.8 3,999,562 + PS417_18360 0.10 -0.7 3,999,597 + PS417_18360 0.21 -1.0 3,999,598 - PS417_18360 0.21 +0.1 3,999,614 - PS417_18360 0.26 +1.0 3,999,857 + -0.6 3,999,857 + +0.8 3,999,857 + -0.5 3,999,857 + +0.2 3,999,858 - +0.3 3,999,916 + -0.4 3,999,917 - +0.3
Or see this region's nucleotide sequence