Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0019 and Echvi_0020 are separated by 351 nucleotides Echvi_0020 and Echvi_0021 are separated by 71 nucleotides Echvi_0021 and Echvi_0022 are separated by 2 nucleotides Echvi_0022 and Echvi_0023 are separated by 4 nucleotides
Echvi_0019: Echvi_0019 - hypothetical protein, at 17,776 to 18,441
_0019
Echvi_0020: Echvi_0020 - conserved hypothetical protein YmdA/YtgF, at 18,793 to 20,352
_0020
Echvi_0021: Echvi_0021 - Cell division protein ZapA., at 20,424 to 20,708
_0021
Echvi_0022: Echvi_0022 - hypothetical protein, at 20,711 to 20,995
_0022
Echvi_0023: Echvi_0023 - phenylalanyl-tRNA synthetase, beta subunit, non-spirochete bacterial, at 21,000 to 23,411
_0023
Position (kb)
18
19
20
21 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 17.866 kb on - strand, within Echvi_0019 at 17.887 kb on + strand, within Echvi_0019 at 17.891 kb on + strand, within Echvi_0019 at 17.891 kb on + strand, within Echvi_0019 at 17.913 kb on + strand, within Echvi_0019 at 17.913 kb on + strand, within Echvi_0019 at 17.914 kb on - strand, within Echvi_0019 at 17.915 kb on + strand, within Echvi_0019 at 17.916 kb on - strand, within Echvi_0019 at 17.949 kb on + strand, within Echvi_0019 at 17.950 kb on - strand, within Echvi_0019 at 17.955 kb on + strand, within Echvi_0019 at 17.956 kb on - strand, within Echvi_0019 at 18.056 kb on - strand, within Echvi_0019 at 18.056 kb on - strand, within Echvi_0019 at 18.058 kb on + strand, within Echvi_0019 at 18.152 kb on + strand, within Echvi_0019 at 18.152 kb on + strand, within Echvi_0019 at 18.153 kb on - strand, within Echvi_0019 at 18.178 kb on - strand, within Echvi_0019 at 18.180 kb on - strand, within Echvi_0019 at 18.276 kb on + strand, within Echvi_0019 at 18.277 kb on - strand, within Echvi_0019 at 18.277 kb on - strand, within Echvi_0019 at 18.336 kb on + strand, within Echvi_0019 at 18.427 kb on - strand at 18.427 kb on - strand at 18.467 kb on + strand at 18.505 kb on - strand at 18.685 kb on - strand at 18.726 kb on - strand at 18.728 kb on - strand at 18.731 kb on + strand at 18.744 kb on + strand at 18.794 kb on + strand at 20.353 kb on - strand at 20.362 kb on - strand at 20.370 kb on - strand at 20.998 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 17,866 - Echvi_0019 0.14 +1.8 17,887 + Echvi_0019 0.17 +1.0 17,891 + Echvi_0019 0.17 -1.0 17,891 + Echvi_0019 0.17 +0.3 17,913 + Echvi_0019 0.21 -0.6 17,913 + Echvi_0019 0.21 +0.8 17,914 - Echvi_0019 0.21 +1.2 17,915 + Echvi_0019 0.21 +0.3 17,916 - Echvi_0019 0.21 +0.4 17,949 + Echvi_0019 0.26 -1.1 17,950 - Echvi_0019 0.26 -2.2 17,955 + Echvi_0019 0.27 +1.3 17,956 - Echvi_0019 0.27 +1.8 18,056 - Echvi_0019 0.42 +0.1 18,056 - Echvi_0019 0.42 -1.2 18,058 + Echvi_0019 0.42 +1.9 18,152 + Echvi_0019 0.56 +0.5 18,152 + Echvi_0019 0.56 +0.0 18,153 - Echvi_0019 0.57 +0.1 18,178 - Echvi_0019 0.60 -0.5 18,180 - Echvi_0019 0.61 -2.3 18,276 + Echvi_0019 0.75 +0.2 18,277 - Echvi_0019 0.75 +1.4 18,277 - Echvi_0019 0.75 -0.7 18,336 + Echvi_0019 0.84 -0.3 18,427 - +0.4 18,427 - -0.5 18,467 + +0.7 18,505 - +1.0 18,685 - -0.3 18,726 - +0.3 18,728 - -1.1 18,731 + -1.0 18,744 + -0.2 18,794 + -0.6 20,353 - -2.5 20,362 - -0.4 20,370 - -0.4 20,998 + +0.7
Or see this region's nucleotide sequence