Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5473 and Atu5474 are separated by 61 nucleotides
Atu5473: Atu5473 - D-alanine aminotransferase, at 465,274 to 466,146
Atu5473
Atu5474: Atu5474 - 2-hydroxyacid dehydrogenase, at 466,208 to 467,143
Atu5474
Position (kb) 465
466
467 Strain fitness (log2 ratio) -1
0
1
2 at 465.235 kb on - strand at 465.328 kb on + strand at 465.358 kb on - strand at 465.360 kb on - strand at 465.406 kb on + strand, within Atu5473 at 465.406 kb on + strand, within Atu5473 at 465.487 kb on + strand, within Atu5473 at 465.487 kb on + strand, within Atu5473 at 465.520 kb on + strand, within Atu5473 at 465.574 kb on + strand, within Atu5473 at 465.607 kb on + strand, within Atu5473 at 465.607 kb on + strand, within Atu5473 at 465.627 kb on + strand, within Atu5473 at 465.770 kb on + strand, within Atu5473 at 465.778 kb on - strand, within Atu5473 at 465.833 kb on + strand, within Atu5473 at 465.847 kb on - strand, within Atu5473 at 465.865 kb on + strand, within Atu5473 at 465.873 kb on - strand, within Atu5473 at 465.881 kb on + strand, within Atu5473 at 465.926 kb on + strand, within Atu5473 at 465.957 kb on + strand, within Atu5473 at 465.969 kb on + strand, within Atu5473 at 466.069 kb on + strand at 466.099 kb on + strand at 466.135 kb on + strand at 466.150 kb on + strand at 466.150 kb on + strand at 466.181 kb on - strand at 466.206 kb on + strand at 466.209 kb on - strand at 466.211 kb on - strand at 466.243 kb on + strand at 466.248 kb on + strand at 466.271 kb on + strand at 466.275 kb on - strand at 466.350 kb on + strand, within Atu5474 at 466.358 kb on - strand, within Atu5474 at 466.358 kb on - strand, within Atu5474 at 466.431 kb on + strand, within Atu5474 at 466.478 kb on + strand, within Atu5474 at 466.491 kb on + strand, within Atu5474 at 466.499 kb on - strand, within Atu5474 at 466.518 kb on + strand, within Atu5474 at 466.536 kb on - strand, within Atu5474 at 466.557 kb on + strand, within Atu5474 at 466.616 kb on + strand, within Atu5474 at 466.690 kb on - strand, within Atu5474 at 466.707 kb on + strand, within Atu5474 at 466.719 kb on - strand, within Atu5474 at 466.752 kb on + strand, within Atu5474 at 466.807 kb on - strand, within Atu5474 at 466.832 kb on + strand, within Atu5474 at 466.835 kb on - strand, within Atu5474 at 466.853 kb on + strand, within Atu5474 at 466.909 kb on + strand, within Atu5474 at 466.922 kb on - strand, within Atu5474 at 466.987 kb on + strand, within Atu5474 at 467.094 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 465,235 - -1.3 465,328 + -0.6 465,358 - -0.4 465,360 - +0.9 465,406 + Atu5473 0.15 -0.4 465,406 + Atu5473 0.15 +0.3 465,487 + Atu5473 0.24 -0.7 465,487 + Atu5473 0.24 +0.1 465,520 + Atu5473 0.28 +1.4 465,574 + Atu5473 0.34 +0.5 465,607 + Atu5473 0.38 +1.3 465,607 + Atu5473 0.38 +0.6 465,627 + Atu5473 0.40 -0.6 465,770 + Atu5473 0.57 -1.4 465,778 - Atu5473 0.58 -0.9 465,833 + Atu5473 0.64 +0.5 465,847 - Atu5473 0.66 +1.2 465,865 + Atu5473 0.68 +0.6 465,873 - Atu5473 0.69 +0.6 465,881 + Atu5473 0.70 -0.5 465,926 + Atu5473 0.75 +1.5 465,957 + Atu5473 0.78 -0.3 465,969 + Atu5473 0.80 +1.3 466,069 + -0.1 466,099 + +0.2 466,135 + -0.2 466,150 + -0.4 466,150 + +0.6 466,181 - +0.3 466,206 + -0.4 466,209 - -0.9 466,211 - +0.4 466,243 + -0.1 466,248 + +0.5 466,271 + -0.6 466,275 - +0.7 466,350 + Atu5474 0.15 -1.1 466,358 - Atu5474 0.16 +0.6 466,358 - Atu5474 0.16 +0.0 466,431 + Atu5474 0.24 -0.0 466,478 + Atu5474 0.29 +0.3 466,491 + Atu5474 0.30 -0.9 466,499 - Atu5474 0.31 +0.0 466,518 + Atu5474 0.33 +0.1 466,536 - Atu5474 0.35 +1.1 466,557 + Atu5474 0.37 -0.3 466,616 + Atu5474 0.44 -0.9 466,690 - Atu5474 0.51 +0.2 466,707 + Atu5474 0.53 -1.5 466,719 - Atu5474 0.55 -0.1 466,752 + Atu5474 0.58 -0.1 466,807 - Atu5474 0.64 -0.4 466,832 + Atu5474 0.67 +2.5 466,835 - Atu5474 0.67 +0.0 466,853 + Atu5474 0.69 -0.1 466,909 + Atu5474 0.75 -0.6 466,922 - Atu5474 0.76 -0.4 466,987 + Atu5474 0.83 +0.1 467,094 + +0.4
Or see this region's nucleotide sequence