Experiment: NAG (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1961 and Atu1962 are separated by 44 nucleotides Atu1962 and Atu1964 are separated by 182 nucleotides Atu1964 and Atu1965 are separated by 127 nucleotides Atu1965 and Atu1966 are separated by 142 nucleotides
Atu1961: Atu1961 - transcription antitermination protein NusG, at 1,934,185 to 1,934,715
Atu1961
Atu1962: Atu1962 - secretion protein, at 1,934,760 to 1,934,960
Atu1962
Atu1964: Atu1964 - tRNA-Trp, at 1,935,143 to 1,935,218
Atu1964
Atu1965: Atu1965 - UDP-glucose 4-epimerase, at 1,935,346 to 1,936,239
Atu1965
Atu1966: Atu1966 - elongation factor TU, at 1,936,382 to 1,937,557
Atu1966
Position (kb)
1935
1936
1937 Strain fitness (log2 ratio)
-1
0
1 at 1935.375 kb on + strand at 1935.433 kb on - strand at 1935.490 kb on + strand, within Atu1965 at 1935.490 kb on + strand, within Atu1965 at 1935.522 kb on - strand, within Atu1965 at 1935.544 kb on - strand, within Atu1965 at 1935.791 kb on + strand, within Atu1965 at 1935.926 kb on - strand, within Atu1965 at 1935.926 kb on - strand, within Atu1965 at 1935.926 kb on - strand, within Atu1965 at 1935.976 kb on + strand, within Atu1965 at 1936.110 kb on + strand, within Atu1965 at 1936.153 kb on - strand at 1936.155 kb on - strand at 1936.211 kb on - strand at 1936.265 kb on - strand at 1936.308 kb on - strand at 1936.313 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (N) remove 1,935,375 + -1.2 1,935,433 - +0.3 1,935,490 + Atu1965 0.16 +0.1 1,935,490 + Atu1965 0.16 +0.8 1,935,522 - Atu1965 0.20 +0.4 1,935,544 - Atu1965 0.22 -0.2 1,935,791 + Atu1965 0.50 -0.0 1,935,926 - Atu1965 0.65 +0.9 1,935,926 - Atu1965 0.65 -0.2 1,935,926 - Atu1965 0.65 +0.4 1,935,976 + Atu1965 0.70 -0.1 1,936,110 + Atu1965 0.85 +1.6 1,936,153 - -0.9 1,936,155 - +0.4 1,936,211 - +0.2 1,936,265 - -0.6 1,936,308 - +0.8 1,936,313 + +0.1
Or see this region's nucleotide sequence