Strain Fitness in Escherichia coli BW25113 around b4209

Experiment: deuterated glucose in D2O

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcycA and ytfE are separated by 44 nucleotidesytfE and ytfF are separated by 107 nucleotides b4208: cycA - D-alanine/D-serine/glycine transporter (NCBI), at 4,427,887 to 4,429,299 cycA b4209: ytfE - predicted regulator of cell morphogenesis and cell wall metabolism (NCBI), at 4,429,344 to 4,430,006 ytfE b4210: ytfF - predicted inner membrane protein (RefSeq), at 4,430,114 to 4,431,079 ytfF Position (kb) 4429 4430 4431Strain fitness (log2 ratio) -2 -1 0 1 2at 4428.375 kb on - strand, within cycAat 4428.398 kb on + strand, within cycAat 4428.422 kb on + strand, within cycAat 4428.427 kb on + strand, within cycAat 4428.454 kb on - strand, within cycAat 4428.589 kb on - strand, within cycAat 4428.690 kb on - strand, within cycAat 4428.692 kb on - strand, within cycAat 4428.742 kb on - strand, within cycAat 4428.752 kb on + strand, within cycAat 4428.752 kb on + strand, within cycAat 4428.765 kb on - strand, within cycAat 4428.765 kb on - strand, within cycAat 4428.793 kb on + strand, within cycAat 4428.793 kb on + strand, within cycAat 4428.793 kb on + strand, within cycAat 4428.793 kb on + strand, within cycAat 4428.793 kb on + strand, within cycAat 4428.793 kb on + strand, within cycAat 4428.850 kb on - strand, within cycAat 4428.880 kb on - strand, within cycAat 4428.980 kb on + strand, within cycAat 4428.980 kb on + strand, within cycAat 4428.985 kb on + strand, within cycAat 4429.009 kb on + strand, within cycAat 4429.087 kb on - strand, within cycAat 4429.088 kb on + strand, within cycAat 4429.111 kb on + strand, within cycAat 4429.156 kb on - strand, within cycAat 4429.159 kb on - strandat 4429.176 kb on - strandat 4429.185 kb on - strandat 4429.195 kb on + strandat 4429.221 kb on - strandat 4429.221 kb on - strandat 4429.254 kb on - strandat 4429.280 kb on + strandat 4429.315 kb on + strandat 4429.361 kb on + strandat 4429.373 kb on + strandat 4429.453 kb on + strand, within ytfEat 4429.453 kb on + strand, within ytfEat 4429.463 kb on - strand, within ytfEat 4429.463 kb on - strand, within ytfEat 4429.466 kb on - strand, within ytfEat 4429.501 kb on - strand, within ytfEat 4429.559 kb on + strand, within ytfEat 4429.559 kb on + strand, within ytfEat 4429.631 kb on - strand, within ytfEat 4429.631 kb on - strand, within ytfEat 4429.798 kb on + strand, within ytfEat 4429.821 kb on + strand, within ytfEat 4429.857 kb on - strand, within ytfEat 4429.907 kb on - strand, within ytfEat 4429.935 kb on - strand, within ytfEat 4429.935 kb on - strand, within ytfEat 4430.003 kb on + strandat 4430.189 kb on - strandat 4430.189 kb on - strandat 4430.189 kb on - strandat 4430.189 kb on - strandat 4430.189 kb on - strandat 4430.196 kb on + strandat 4430.199 kb on - strandat 4430.209 kb on - strandat 4430.320 kb on + strand, within ytfFat 4430.335 kb on - strand, within ytfFat 4430.335 kb on - strand, within ytfFat 4430.408 kb on - strand, within ytfFat 4430.456 kb on - strand, within ytfFat 4430.557 kb on + strand, within ytfFat 4430.565 kb on - strand, within ytfFat 4430.565 kb on - strand, within ytfFat 4430.591 kb on + strand, within ytfFat 4430.739 kb on + strand, within ytfFat 4430.739 kb on + strand, within ytfFat 4430.744 kb on + strand, within ytfFat 4430.744 kb on + strand, within ytfFat 4430.809 kb on + strand, within ytfFat 4430.809 kb on + strand, within ytfFat 4430.849 kb on - strand, within ytfFat 4430.849 kb on - strand, within ytfFat 4430.853 kb on + strand, within ytfFat 4430.853 kb on + strand, within ytfFat 4430.861 kb on - strand, within ytfFat 4430.861 kb on - strand, within ytfFat 4430.878 kb on - strand, within ytfFat 4430.887 kb on - strand, within ytfFat 4430.942 kb on - strand, within ytfFat 4430.955 kb on - strand, within ytfFat 4430.955 kb on - strand, within ytfFat 4430.956 kb on - strand, within ytfFat 4430.988 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction deuterated glucose in D2O
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4,428,375 - cycA b4208 0.35 -0.7
4,428,398 + cycA b4208 0.36 +1.4
4,428,422 + cycA b4208 0.38 +0.3
4,428,427 + cycA b4208 0.38 +0.1
4,428,454 - cycA b4208 0.40 -0.4
4,428,589 - cycA b4208 0.50 +0.1
4,428,690 - cycA b4208 0.57 -0.4
4,428,692 - cycA b4208 0.57 +0.1
4,428,742 - cycA b4208 0.61 +0.1
4,428,752 + cycA b4208 0.61 +0.2
4,428,752 + cycA b4208 0.61 +1.8
4,428,765 - cycA b4208 0.62 -1.9
4,428,765 - cycA b4208 0.62 -1.5
4,428,793 + cycA b4208 0.64 -1.2
4,428,793 + cycA b4208 0.64 +0.3
4,428,793 + cycA b4208 0.64 -0.1
4,428,793 + cycA b4208 0.64 +0.1
4,428,793 + cycA b4208 0.64 -0.1
4,428,793 + cycA b4208 0.64 +0.3
4,428,850 - cycA b4208 0.68 +0.1
4,428,880 - cycA b4208 0.70 +0.3
4,428,980 + cycA b4208 0.77 -0.2
4,428,980 + cycA b4208 0.77 +0.8
4,428,985 + cycA b4208 0.78 -1.2
4,429,009 + cycA b4208 0.79 +0.2
4,429,087 - cycA b4208 0.85 +0.2
4,429,088 + cycA b4208 0.85 +0.4
4,429,111 + cycA b4208 0.87 -1.2
4,429,156 - cycA b4208 0.90 +0.7
4,429,159 - -0.5
4,429,176 - +0.1
4,429,185 - -0.8
4,429,195 + +0.6
4,429,221 - +0.5
4,429,221 - +0.7
4,429,254 - +0.9
4,429,280 + -0.8
4,429,315 + +0.0
4,429,361 + +0.3
4,429,373 + -0.1
4,429,453 + ytfE b4209 0.16 -0.4
4,429,453 + ytfE b4209 0.16 -1.1
4,429,463 - ytfE b4209 0.18 -0.1
4,429,463 - ytfE b4209 0.18 +0.8
4,429,466 - ytfE b4209 0.18 -1.1
4,429,501 - ytfE b4209 0.24 -0.3
4,429,559 + ytfE b4209 0.32 -1.6
4,429,559 + ytfE b4209 0.32 +0.4
4,429,631 - ytfE b4209 0.43 -1.7
4,429,631 - ytfE b4209 0.43 -0.7
4,429,798 + ytfE b4209 0.68 -0.9
4,429,821 + ytfE b4209 0.72 +0.1
4,429,857 - ytfE b4209 0.77 +0.5
4,429,907 - ytfE b4209 0.85 -0.2
4,429,935 - ytfE b4209 0.89 -1.1
4,429,935 - ytfE b4209 0.89 -1.9
4,430,003 + +1.7
4,430,189 - +0.1
4,430,189 - +0.1
4,430,189 - -0.2
4,430,189 - -0.1
4,430,189 - +0.2
4,430,196 + +1.2
4,430,199 - +0.1
4,430,209 - +0.6
4,430,320 + ytfF b4210 0.21 -0.6
4,430,335 - ytfF b4210 0.23 -1.3
4,430,335 - ytfF b4210 0.23 -0.6
4,430,408 - ytfF b4210 0.30 +0.3
4,430,456 - ytfF b4210 0.35 +0.2
4,430,557 + ytfF b4210 0.46 -0.3
4,430,565 - ytfF b4210 0.47 -0.0
4,430,565 - ytfF b4210 0.47 +0.4
4,430,591 + ytfF b4210 0.49 +0.1
4,430,739 + ytfF b4210 0.65 +0.4
4,430,739 + ytfF b4210 0.65 -0.3
4,430,744 + ytfF b4210 0.65 +1.2
4,430,744 + ytfF b4210 0.65 -0.7
4,430,809 + ytfF b4210 0.72 +0.0
4,430,809 + ytfF b4210 0.72 -0.3
4,430,849 - ytfF b4210 0.76 -0.1
4,430,849 - ytfF b4210 0.76 -0.6
4,430,853 + ytfF b4210 0.77 -0.3
4,430,853 + ytfF b4210 0.77 +0.2
4,430,861 - ytfF b4210 0.77 +1.8
4,430,861 - ytfF b4210 0.77 +1.6
4,430,878 - ytfF b4210 0.79 -2.4
4,430,887 - ytfF b4210 0.80 +0.1
4,430,942 - ytfF b4210 0.86 -0.7
4,430,955 - ytfF b4210 0.87 +0.4
4,430,955 - ytfF b4210 0.87 -0.2
4,430,956 - ytfF b4210 0.87 -0.6
4,430,988 - -0.0

Or see this region's nucleotide sequence