Experiment: Chloramphenicol 0.015 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12725 and PS417_12730 are separated by 72 nucleotides PS417_12730 and PS417_12735 are separated by 158 nucleotides PS417_12735 and PS417_12740 are separated by 232 nucleotides
PS417_12725: PS417_12725 - AraC family transcriptional regulator, at 2,774,756 to 2,775,703
_12725
PS417_12730: PS417_12730 - glycosyltransferase, at 2,775,776 to 2,777,065
_12730
PS417_12735: PS417_12735 - peroxidase, at 2,777,224 to 2,777,724
_12735
PS417_12740: PS417_12740 - secretion protein HlyD, at 2,777,957 to 2,779,258
_12740
Position (kb)
2775
2776
2777
2778 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2774.903 kb on - strand, within PS417_12725 at 2775.063 kb on + strand, within PS417_12725 at 2775.115 kb on - strand, within PS417_12725 at 2775.525 kb on + strand, within PS417_12725 at 2775.670 kb on - strand at 2775.738 kb on + strand at 2775.780 kb on - strand at 2775.816 kb on + strand at 2775.816 kb on + strand at 2775.816 kb on + strand at 2775.816 kb on + strand at 2775.816 kb on + strand at 2775.816 kb on + strand at 2775.817 kb on - strand at 2775.817 kb on - strand at 2775.817 kb on - strand at 2775.817 kb on - strand at 2775.830 kb on - strand at 2776.297 kb on - strand, within PS417_12730 at 2776.297 kb on - strand at 2776.987 kb on + strand at 2777.151 kb on + strand at 2777.210 kb on + strand at 2777.211 kb on - strand at 2777.362 kb on - strand, within PS417_12735 at 2777.691 kb on + strand at 2777.695 kb on + strand at 2777.722 kb on + strand at 2777.722 kb on + strand at 2777.723 kb on - strand at 2777.755 kb on + strand at 2777.851 kb on + strand at 2777.852 kb on - strand at 2777.866 kb on - strand at 2777.876 kb on + strand at 2777.877 kb on - strand at 2777.880 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.015 mg/ml remove 2,774,903 - PS417_12725 0.16 +0.1 2,775,063 + PS417_12725 0.32 -0.4 2,775,115 - PS417_12725 0.38 +0.9 2,775,525 + PS417_12725 0.81 +0.2 2,775,670 - -3.0 2,775,738 + +0.6 2,775,780 - -0.1 2,775,816 + +0.2 2,775,816 + -1.3 2,775,816 + +0.4 2,775,816 + -1.0 2,775,816 + +0.0 2,775,816 + +0.2 2,775,817 - -0.1 2,775,817 - -0.6 2,775,817 - -0.7 2,775,817 - -0.6 2,775,830 - -0.7 2,776,297 - PS417_12730 0.40 -0.6 2,776,297 - -0.1 2,776,987 + -0.3 2,777,151 + +0.6 2,777,210 + +0.1 2,777,211 - +0.0 2,777,362 - PS417_12735 0.28 +0.4 2,777,691 + +0.1 2,777,695 + -0.2 2,777,722 + -0.4 2,777,722 + +0.4 2,777,723 - +1.3 2,777,755 + -0.1 2,777,851 + -0.4 2,777,852 - +0.2 2,777,866 - +0.6 2,777,876 + +0.2 2,777,877 - -0.1 2,777,880 + +0.3
Or see this region's nucleotide sequence