Experiment: deuterated glucose in D2O
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt livM and livH overlap by 4 nucleotides livH and livK are separated by 47 nucleotides livK and yhhK are separated by 423 nucleotides yhhK and livJ are separated by 187 nucleotides
b3456: livM - leucine/isoleucine/valine transporter subunit (NCBI), at 3,592,226 to 3,593,503
livM
b3457: livH - leucine/isoleucine/valine transporter subunit (NCBI), at 3,593,500 to 3,594,426
livH
b3458: livK - leucine transporter subunit (NCBI), at 3,594,474 to 3,595,583
livK
b3459: yhhK - hypothetical protein (NCBI), at 3,596,007 to 3,596,390
yhhK
b3460: livJ - leucine/isoleucine/valine transporter subunit (NCBI), at 3,596,578 to 3,597,681
livJ
Position (kb)
3594
3595
3596 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 3593.524 kb on + strand at 3593.620 kb on - strand, within livH at 3593.620 kb on - strand, within livH at 3593.655 kb on - strand, within livH at 3593.655 kb on - strand, within livH at 3593.655 kb on - strand, within livH at 3593.655 kb on - strand, within livH at 3593.660 kb on + strand, within livH at 3593.660 kb on + strand, within livH at 3593.749 kb on + strand, within livH at 3593.825 kb on + strand, within livH at 3593.833 kb on - strand, within livH at 3593.869 kb on + strand, within livH at 3593.942 kb on + strand, within livH at 3593.942 kb on + strand, within livH at 3593.966 kb on - strand, within livH at 3594.055 kb on - strand, within livH at 3594.098 kb on - strand, within livH at 3594.150 kb on + strand, within livH at 3594.150 kb on + strand, within livH at 3594.220 kb on + strand, within livH at 3594.232 kb on - strand, within livH at 3594.232 kb on - strand, within livH at 3594.236 kb on + strand, within livH at 3594.236 kb on + strand, within livH at 3594.346 kb on - strand at 3594.350 kb on - strand at 3594.350 kb on - strand at 3594.404 kb on - strand at 3594.404 kb on - strand at 3594.417 kb on + strand at 3594.469 kb on + strand at 3594.485 kb on - strand at 3594.518 kb on - strand at 3594.593 kb on + strand, within livK at 3594.638 kb on + strand, within livK at 3594.639 kb on + strand, within livK at 3594.639 kb on + strand, within livK at 3594.639 kb on + strand, within livK at 3594.639 kb on + strand, within livK at 3594.743 kb on - strand, within livK at 3594.821 kb on + strand, within livK at 3594.876 kb on + strand, within livK at 3594.876 kb on + strand, within livK at 3594.908 kb on + strand, within livK at 3594.954 kb on + strand, within livK at 3595.035 kb on + strand, within livK at 3595.043 kb on - strand, within livK at 3595.053 kb on - strand, within livK at 3595.097 kb on + strand, within livK at 3595.109 kb on - strand, within livK at 3595.142 kb on + strand, within livK at 3595.199 kb on + strand, within livK at 3595.207 kb on - strand, within livK at 3595.207 kb on - strand, within livK at 3595.207 kb on - strand, within livK at 3595.264 kb on + strand, within livK at 3595.276 kb on - strand, within livK at 3595.343 kb on - strand, within livK at 3595.343 kb on - strand, within livK at 3595.354 kb on - strand, within livK at 3595.365 kb on + strand, within livK at 3595.427 kb on - strand, within livK at 3595.466 kb on + strand, within livK at 3595.466 kb on + strand, within livK at 3595.510 kb on - strand at 3595.517 kb on + strand at 3595.517 kb on + strand at 3595.549 kb on - strand at 3595.553 kb on - strand at 3595.553 kb on - strand at 3595.554 kb on + strand at 3595.637 kb on + strand at 3595.669 kb on + strand at 3595.968 kb on + strand at 3595.975 kb on + strand at 3596.005 kb on + strand at 3596.005 kb on - strand at 3596.005 kb on - strand at 3596.007 kb on + strand at 3596.011 kb on - strand at 3596.012 kb on - strand at 3596.013 kb on - strand at 3596.043 kb on + strand at 3596.048 kb on + strand, within yhhK at 3596.048 kb on + strand, within yhhK at 3596.056 kb on - strand, within yhhK at 3596.056 kb on - strand, within yhhK at 3596.061 kb on - strand, within yhhK at 3596.117 kb on + strand, within yhhK at 3596.128 kb on - strand, within yhhK at 3596.163 kb on - strand, within yhhK at 3596.163 kb on - strand, within yhhK at 3596.169 kb on + strand, within yhhK at 3596.209 kb on + strand, within yhhK at 3596.209 kb on + strand, within yhhK at 3596.251 kb on - strand, within yhhK at 3596.251 kb on - strand, within yhhK at 3596.267 kb on + strand, within yhhK at 3596.267 kb on + strand, within yhhK at 3596.288 kb on + strand, within yhhK at 3596.334 kb on + strand, within yhhK at 3596.420 kb on + strand at 3596.427 kb on + strand at 3596.427 kb on + strand at 3596.500 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction deuterated glucose in D2O remove 3,593,524 + +4.7 3,593,620 - livH b3457 0.13 +4.5 3,593,620 - livH b3457 0.13 +4.3 3,593,655 - livH b3457 0.17 +4.3 3,593,655 - livH b3457 0.17 +5.4 3,593,655 - livH b3457 0.17 +4.6 3,593,655 - livH b3457 0.17 +4.4 3,593,660 + livH b3457 0.17 +5.0 3,593,660 + livH b3457 0.17 +4.9 3,593,749 + livH b3457 0.27 +4.6 3,593,825 + livH b3457 0.35 +6.1 3,593,833 - livH b3457 0.36 +5.0 3,593,869 + livH b3457 0.40 +4.7 3,593,942 + livH b3457 0.48 +5.2 3,593,942 + livH b3457 0.48 +5.3 3,593,966 - livH b3457 0.50 +5.0 3,594,055 - livH b3457 0.60 +5.0 3,594,098 - livH b3457 0.65 +5.0 3,594,150 + livH b3457 0.70 +5.1 3,594,150 + livH b3457 0.70 +5.9 3,594,220 + livH b3457 0.78 +4.7 3,594,232 - livH b3457 0.79 +5.3 3,594,232 - livH b3457 0.79 +5.0 3,594,236 + livH b3457 0.79 +6.2 3,594,236 + livH b3457 0.79 +4.5 3,594,346 - +4.8 3,594,350 - +4.4 3,594,350 - +4.0 3,594,404 - +5.2 3,594,404 - +5.6 3,594,417 + +5.5 3,594,469 + +4.9 3,594,485 - -3.3 3,594,518 - -3.6 3,594,593 + livK b3458 0.11 +2.0 3,594,638 + livK b3458 0.15 +1.6 3,594,639 + livK b3458 0.15 +3.8 3,594,639 + livK b3458 0.15 +3.1 3,594,639 + livK b3458 0.15 +3.5 3,594,639 + livK b3458 0.15 +2.8 3,594,743 - livK b3458 0.24 -2.7 3,594,821 + livK b3458 0.31 +2.2 3,594,876 + livK b3458 0.36 +3.3 3,594,876 + livK b3458 0.36 +4.0 3,594,908 + livK b3458 0.39 +3.8 3,594,954 + livK b3458 0.43 +3.6 3,595,035 + livK b3458 0.51 +4.0 3,595,043 - livK b3458 0.51 -2.0 3,595,053 - livK b3458 0.52 -1.9 3,595,097 + livK b3458 0.56 +3.8 3,595,109 - livK b3458 0.57 -0.1 3,595,142 + livK b3458 0.60 +3.8 3,595,199 + livK b3458 0.65 +5.6 3,595,207 - livK b3458 0.66 +2.7 3,595,207 - livK b3458 0.66 +2.6 3,595,207 - livK b3458 0.66 +2.1 3,595,264 + livK b3458 0.71 +3.5 3,595,276 - livK b3458 0.72 +4.2 3,595,343 - livK b3458 0.78 +1.8 3,595,343 - livK b3458 0.78 +3.8 3,595,354 - livK b3458 0.79 +3.9 3,595,365 + livK b3458 0.80 +4.3 3,595,427 - livK b3458 0.86 +1.7 3,595,466 + livK b3458 0.89 +4.9 3,595,466 + livK b3458 0.89 +4.1 3,595,510 - +3.4 3,595,517 + +4.7 3,595,517 + +4.6 3,595,549 - +1.5 3,595,553 - -0.3 3,595,553 - -1.3 3,595,554 + +5.0 3,595,637 + +2.0 3,595,669 + +2.2 3,595,968 + +0.8 3,595,975 + +1.4 3,596,005 + +3.1 3,596,005 - -0.5 3,596,005 - +1.3 3,596,007 + -0.2 3,596,011 - +0.0 3,596,012 - -0.4 3,596,013 - -0.0 3,596,043 + +1.3 3,596,048 + yhhK b3459 0.11 +2.0 3,596,048 + yhhK b3459 0.11 +2.0 3,596,056 - yhhK b3459 0.13 +0.9 3,596,056 - yhhK b3459 0.13 +1.0 3,596,061 - yhhK b3459 0.14 +1.0 3,596,117 + yhhK b3459 0.29 +2.0 3,596,128 - yhhK b3459 0.32 +0.4 3,596,163 - yhhK b3459 0.41 -0.0 3,596,163 - yhhK b3459 0.41 -0.7 3,596,169 + yhhK b3459 0.42 +0.6 3,596,209 + yhhK b3459 0.53 +0.9 3,596,209 + yhhK b3459 0.53 +1.2 3,596,251 - yhhK b3459 0.64 +1.4 3,596,251 - yhhK b3459 0.64 -0.1 3,596,267 + yhhK b3459 0.68 -0.2 3,596,267 + yhhK b3459 0.68 +0.1 3,596,288 + yhhK b3459 0.73 +0.6 3,596,334 + yhhK b3459 0.85 +0.8 3,596,420 + -0.5 3,596,427 + +0.3 3,596,427 + -0.6 3,596,500 + +0.9
Or see this region's nucleotide sequence