Experiment: deuterated glucose in D2O
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yrfG and hslR are separated by 10 nucleotides hslR and yrfI are separated by 24 nucleotides yrfI and yhgE are separated by 62 nucleotides
b3399: yrfG - putative phosphatase (VIMSS), at 3,526,691 to 3,527,359
yrfG
b3400: hslR - ribosome-associated heat shock protein Hsp15 (NCBI), at 3,527,370 to 3,527,771
hslR
b3401: yrfI - orf, hypothetical protein (VIMSS), at 3,527,796 to 3,528,674
yrfI
b3402: yhgE - predicted inner membrane protein (NCBI), at 3,528,737 to 3,530,461
yhgE
Position (kb)
3527
3528
3529 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3526.805 kb on - strand, within yrfG at 3526.857 kb on + strand, within yrfG at 3526.859 kb on + strand, within yrfG at 3526.859 kb on + strand, within yrfG at 3526.876 kb on + strand, within yrfG at 3526.876 kb on + strand, within yrfG at 3526.880 kb on + strand, within yrfG at 3526.880 kb on + strand, within yrfG at 3526.884 kb on - strand, within yrfG at 3526.888 kb on - strand, within yrfG at 3526.888 kb on - strand, within yrfG at 3526.894 kb on - strand, within yrfG at 3526.899 kb on - strand, within yrfG at 3526.899 kb on - strand, within yrfG at 3526.911 kb on - strand, within yrfG at 3526.911 kb on - strand, within yrfG at 3526.921 kb on - strand, within yrfG at 3526.954 kb on - strand, within yrfG at 3526.954 kb on - strand, within yrfG at 3526.962 kb on + strand, within yrfG at 3526.973 kb on + strand, within yrfG at 3526.990 kb on + strand, within yrfG at 3527.045 kb on - strand, within yrfG at 3527.048 kb on + strand, within yrfG at 3527.055 kb on + strand, within yrfG at 3527.056 kb on - strand, within yrfG at 3527.079 kb on - strand, within yrfG at 3527.079 kb on - strand, within yrfG at 3527.081 kb on + strand, within yrfG at 3527.089 kb on - strand, within yrfG at 3527.162 kb on - strand, within yrfG at 3527.162 kb on - strand, within yrfG at 3527.200 kb on + strand, within yrfG at 3527.207 kb on + strand, within yrfG at 3527.207 kb on + strand, within yrfG at 3527.212 kb on + strand, within yrfG at 3527.214 kb on + strand, within yrfG at 3527.259 kb on + strand, within yrfG at 3527.259 kb on + strand, within yrfG at 3527.313 kb on + strand at 3527.332 kb on - strand at 3527.372 kb on - strand at 3527.372 kb on - strand at 3527.383 kb on + strand at 3527.392 kb on + strand at 3527.411 kb on + strand, within hslR at 3527.411 kb on + strand, within hslR at 3527.414 kb on - strand, within hslR at 3527.414 kb on - strand, within hslR at 3527.419 kb on - strand, within hslR at 3527.424 kb on - strand, within hslR at 3527.442 kb on + strand, within hslR at 3527.442 kb on + strand, within hslR at 3527.449 kb on + strand, within hslR at 3527.475 kb on + strand, within hslR at 3527.475 kb on + strand, within hslR at 3527.483 kb on - strand, within hslR at 3527.492 kb on + strand, within hslR at 3527.530 kb on + strand, within hslR at 3527.538 kb on - strand, within hslR at 3527.538 kb on - strand, within hslR at 3527.538 kb on - strand, within hslR at 3527.538 kb on - strand, within hslR at 3527.548 kb on + strand, within hslR at 3527.556 kb on - strand, within hslR at 3527.565 kb on + strand, within hslR at 3527.565 kb on + strand, within hslR at 3527.589 kb on - strand, within hslR at 3527.608 kb on - strand, within hslR at 3527.628 kb on - strand, within hslR at 3527.632 kb on - strand, within hslR at 3527.632 kb on - strand, within hslR at 3527.641 kb on + strand, within hslR at 3527.649 kb on - strand, within hslR at 3527.649 kb on - strand, within hslR at 3527.679 kb on + strand, within hslR at 3527.685 kb on + strand, within hslR at 3527.690 kb on + strand, within hslR at 3527.698 kb on - strand, within hslR at 3527.698 kb on - strand, within hslR at 3527.764 kb on + strand at 3527.772 kb on - strand at 3527.772 kb on - strand at 3527.784 kb on + strand at 3527.787 kb on - strand at 3527.789 kb on - strand at 3527.883 kb on - strand at 3527.887 kb on + strand, within yrfI at 3527.902 kb on + strand, within yrfI at 3527.930 kb on + strand, within yrfI at 3527.943 kb on + strand, within yrfI at 3527.964 kb on - strand, within yrfI at 3527.964 kb on - strand, within yrfI at 3527.984 kb on + strand, within yrfI at 3527.994 kb on + strand, within yrfI at 3528.007 kb on - strand, within yrfI at 3528.007 kb on - strand, within yrfI at 3528.051 kb on + strand, within yrfI at 3528.062 kb on - strand, within yrfI at 3528.064 kb on + strand, within yrfI at 3528.142 kb on + strand, within yrfI at 3528.150 kb on - strand, within yrfI at 3528.150 kb on - strand, within yrfI at 3528.150 kb on - strand, within yrfI at 3528.167 kb on + strand, within yrfI at 3528.174 kb on + strand, within yrfI at 3528.191 kb on - strand, within yrfI at 3528.208 kb on - strand, within yrfI at 3528.211 kb on + strand, within yrfI at 3528.219 kb on - strand, within yrfI at 3528.231 kb on - strand, within yrfI at 3528.242 kb on + strand, within yrfI at 3528.311 kb on - strand, within yrfI at 3528.314 kb on + strand, within yrfI at 3528.356 kb on + strand, within yrfI at 3528.409 kb on + strand, within yrfI at 3528.409 kb on + strand, within yrfI at 3528.476 kb on + strand, within yrfI at 3528.476 kb on + strand, within yrfI at 3528.476 kb on + strand, within yrfI at 3528.480 kb on + strand, within yrfI at 3528.515 kb on + strand, within yrfI at 3528.548 kb on - strand, within yrfI at 3528.553 kb on + strand, within yrfI at 3528.583 kb on + strand, within yrfI at 3528.627 kb on + strand at 3528.627 kb on + strand at 3528.655 kb on - strand at 3528.676 kb on - strand at 3528.695 kb on - strand at 3528.695 kb on - strand at 3528.715 kb on - strand at 3528.738 kb on - strand at 3528.738 kb on - strand at 3528.768 kb on - strand at 3528.768 kb on - strand at 3528.778 kb on - strand at 3528.831 kb on - strand at 3528.870 kb on - strand at 3528.879 kb on + strand at 3528.895 kb on - strand at 3528.895 kb on - strand at 3528.917 kb on + strand, within yhgE at 3528.925 kb on - strand, within yhgE at 3528.962 kb on - strand, within yhgE at 3528.969 kb on + strand, within yhgE at 3528.972 kb on + strand, within yhgE at 3529.009 kb on + strand, within yhgE at 3529.030 kb on - strand, within yhgE at 3529.035 kb on - strand, within yhgE at 3529.035 kb on - strand, within yhgE at 3529.043 kb on + strand, within yhgE at 3529.043 kb on + strand, within yhgE at 3529.045 kb on - strand, within yhgE at 3529.070 kb on + strand, within yhgE at 3529.070 kb on + strand, within yhgE at 3529.070 kb on + strand, within yhgE at 3529.070 kb on + strand, within yhgE at 3529.088 kb on - strand, within yhgE at 3529.135 kb on - strand, within yhgE at 3529.135 kb on - strand, within yhgE at 3529.138 kb on - strand, within yhgE at 3529.173 kb on - strand, within yhgE at 3529.173 kb on - strand, within yhgE at 3529.206 kb on + strand, within yhgE at 3529.228 kb on - strand, within yhgE at 3529.252 kb on + strand, within yhgE at 3529.252 kb on + strand, within yhgE at 3529.260 kb on - strand, within yhgE at 3529.260 kb on - strand, within yhgE at 3529.261 kb on + strand, within yhgE at 3529.261 kb on + strand, within yhgE at 3529.267 kb on + strand, within yhgE at 3529.317 kb on - strand, within yhgE at 3529.338 kb on - strand, within yhgE at 3529.338 kb on - strand, within yhgE at 3529.340 kb on + strand, within yhgE at 3529.340 kb on + strand, within yhgE at 3529.348 kb on - strand, within yhgE at 3529.348 kb on - strand, within yhgE at 3529.377 kb on - strand, within yhgE at 3529.377 kb on - strand, within yhgE at 3529.377 kb on - strand, within yhgE at 3529.377 kb on - strand, within yhgE at 3529.437 kb on + strand, within yhgE at 3529.443 kb on - strand, within yhgE at 3529.457 kb on - strand, within yhgE at 3529.470 kb on + strand, within yhgE at 3529.511 kb on + strand, within yhgE at 3529.511 kb on + strand, within yhgE at 3529.532 kb on - strand, within yhgE at 3529.534 kb on - strand, within yhgE at 3529.537 kb on - strand, within yhgE at 3529.537 kb on - strand, within yhgE at 3529.592 kb on - strand, within yhgE at 3529.626 kb on + strand, within yhgE at 3529.626 kb on + strand, within yhgE at 3529.626 kb on + strand, within yhgE at 3529.626 kb on + strand, within yhgE at 3529.639 kb on - strand, within yhgE
Per-strain Table
Position Strand Gene LocusTag Fraction deuterated glucose in D2O remove 3,526,805 - yrfG b3399 0.17 -0.3 3,526,857 + yrfG b3399 0.25 -0.2 3,526,859 + yrfG b3399 0.25 +3.3 3,526,859 + yrfG b3399 0.25 +0.6 3,526,876 + yrfG b3399 0.28 +1.0 3,526,876 + yrfG b3399 0.28 +0.2 3,526,880 + yrfG b3399 0.28 +0.3 3,526,880 + yrfG b3399 0.28 -1.0 3,526,884 - yrfG b3399 0.29 +2.0 3,526,888 - yrfG b3399 0.29 -0.1 3,526,888 - yrfG b3399 0.29 -0.0 3,526,894 - yrfG b3399 0.30 +0.2 3,526,899 - yrfG b3399 0.31 -0.5 3,526,899 - yrfG b3399 0.31 -0.7 3,526,911 - yrfG b3399 0.33 +0.0 3,526,911 - yrfG b3399 0.33 -0.5 3,526,921 - yrfG b3399 0.34 +1.1 3,526,954 - yrfG b3399 0.39 -0.2 3,526,954 - yrfG b3399 0.39 -0.3 3,526,962 + yrfG b3399 0.41 -0.2 3,526,973 + yrfG b3399 0.42 +0.8 3,526,990 + yrfG b3399 0.45 +0.3 3,527,045 - yrfG b3399 0.53 +0.2 3,527,048 + yrfG b3399 0.53 +0.4 3,527,055 + yrfG b3399 0.54 +0.2 3,527,056 - yrfG b3399 0.55 -0.6 3,527,079 - yrfG b3399 0.58 -0.6 3,527,079 - yrfG b3399 0.58 -0.0 3,527,081 + yrfG b3399 0.58 +0.0 3,527,089 - yrfG b3399 0.59 -0.9 3,527,162 - yrfG b3399 0.70 +2.0 3,527,162 - yrfG b3399 0.70 +0.9 3,527,200 + yrfG b3399 0.76 +0.0 3,527,207 + yrfG b3399 0.77 -0.3 3,527,207 + yrfG b3399 0.77 +0.2 3,527,212 + yrfG b3399 0.78 +0.4 3,527,214 + yrfG b3399 0.78 +1.3 3,527,259 + yrfG b3399 0.85 +0.1 3,527,259 + yrfG b3399 0.85 +0.6 3,527,313 + +1.0 3,527,332 - -0.1 3,527,372 - +0.0 3,527,372 - -0.7 3,527,383 + +1.0 3,527,392 + -0.3 3,527,411 + hslR b3400 0.10 +1.3 3,527,411 + hslR b3400 0.10 +1.0 3,527,414 - hslR b3400 0.11 -0.0 3,527,414 - hslR b3400 0.11 +0.4 3,527,419 - hslR b3400 0.12 -0.5 3,527,424 - hslR b3400 0.13 +0.8 3,527,442 + hslR b3400 0.18 +0.5 3,527,442 + hslR b3400 0.18 -0.5 3,527,449 + hslR b3400 0.20 +1.0 3,527,475 + hslR b3400 0.26 -0.1 3,527,475 + hslR b3400 0.26 +0.6 3,527,483 - hslR b3400 0.28 +0.8 3,527,492 + hslR b3400 0.30 +1.1 3,527,530 + hslR b3400 0.40 +0.5 3,527,538 - hslR b3400 0.42 +0.9 3,527,538 - hslR b3400 0.42 +1.5 3,527,538 - hslR b3400 0.42 +1.3 3,527,538 - hslR b3400 0.42 +0.2 3,527,548 + hslR b3400 0.44 -0.2 3,527,556 - hslR b3400 0.46 +0.2 3,527,565 + hslR b3400 0.49 +0.2 3,527,565 + hslR b3400 0.49 +0.8 3,527,589 - hslR b3400 0.54 +1.4 3,527,608 - hslR b3400 0.59 -0.3 3,527,628 - hslR b3400 0.64 +0.2 3,527,632 - hslR b3400 0.65 +2.3 3,527,632 - hslR b3400 0.65 +1.6 3,527,641 + hslR b3400 0.67 +0.8 3,527,649 - hslR b3400 0.69 -0.0 3,527,649 - hslR b3400 0.69 +0.6 3,527,679 + hslR b3400 0.77 +0.7 3,527,685 + hslR b3400 0.78 +0.7 3,527,690 + hslR b3400 0.80 +0.2 3,527,698 - hslR b3400 0.82 -0.9 3,527,698 - hslR b3400 0.82 -0.8 3,527,764 + +0.4 3,527,772 - +0.5 3,527,772 - -0.2 3,527,784 + +0.0 3,527,787 - +0.7 3,527,789 - +0.3 3,527,883 - +2.0 3,527,887 + yrfI b3401 0.10 -0.3 3,527,902 + yrfI b3401 0.12 +1.3 3,527,930 + yrfI b3401 0.15 +1.1 3,527,943 + yrfI b3401 0.17 +0.9 3,527,964 - yrfI b3401 0.19 +1.6 3,527,964 - yrfI b3401 0.19 +2.3 3,527,984 + yrfI b3401 0.21 +1.6 3,527,994 + yrfI b3401 0.23 +1.5 3,528,007 - yrfI b3401 0.24 +0.6 3,528,007 - yrfI b3401 0.24 +0.5 3,528,051 + yrfI b3401 0.29 +2.0 3,528,062 - yrfI b3401 0.30 -0.0 3,528,064 + yrfI b3401 0.30 +1.0 3,528,142 + yrfI b3401 0.39 +1.0 3,528,150 - yrfI b3401 0.40 +1.4 3,528,150 - yrfI b3401 0.40 +1.6 3,528,150 - yrfI b3401 0.40 +1.7 3,528,167 + yrfI b3401 0.42 +1.4 3,528,174 + yrfI b3401 0.43 +1.5 3,528,191 - yrfI b3401 0.45 +0.5 3,528,208 - yrfI b3401 0.47 +0.8 3,528,211 + yrfI b3401 0.47 +0.8 3,528,219 - yrfI b3401 0.48 +1.1 3,528,231 - yrfI b3401 0.49 +1.2 3,528,242 + yrfI b3401 0.51 +1.8 3,528,311 - yrfI b3401 0.59 +2.0 3,528,314 + yrfI b3401 0.59 +0.3 3,528,356 + yrfI b3401 0.64 +1.4 3,528,409 + yrfI b3401 0.70 +0.6 3,528,409 + yrfI b3401 0.70 +0.7 3,528,476 + yrfI b3401 0.77 +2.0 3,528,476 + yrfI b3401 0.77 +0.6 3,528,476 + yrfI b3401 0.77 +0.4 3,528,480 + yrfI b3401 0.78 +1.1 3,528,515 + yrfI b3401 0.82 +1.3 3,528,548 - yrfI b3401 0.86 +1.4 3,528,553 + yrfI b3401 0.86 -0.0 3,528,583 + yrfI b3401 0.90 +0.9 3,528,627 + +0.9 3,528,627 + +0.3 3,528,655 - -0.3 3,528,676 - +2.0 3,528,695 - -0.1 3,528,695 - +0.3 3,528,715 - +1.1 3,528,738 - -1.0 3,528,738 - -1.3 3,528,768 - -1.0 3,528,768 - -0.8 3,528,778 - +0.1 3,528,831 - +1.1 3,528,870 - +0.6 3,528,879 + -0.5 3,528,895 - -0.0 3,528,895 - +0.4 3,528,917 + yhgE b3402 0.10 +0.3 3,528,925 - yhgE b3402 0.11 -0.6 3,528,962 - yhgE b3402 0.13 +0.5 3,528,969 + yhgE b3402 0.13 -0.7 3,528,972 + yhgE b3402 0.14 +0.8 3,529,009 + yhgE b3402 0.16 +0.5 3,529,030 - yhgE b3402 0.17 +0.6 3,529,035 - yhgE b3402 0.17 -0.0 3,529,035 - yhgE b3402 0.17 +2.0 3,529,043 + yhgE b3402 0.18 -2.3 3,529,043 + yhgE b3402 0.18 +0.8 3,529,045 - yhgE b3402 0.18 +0.4 3,529,070 + yhgE b3402 0.19 -1.1 3,529,070 + yhgE b3402 0.19 +0.3 3,529,070 + yhgE b3402 0.19 +0.6 3,529,070 + yhgE b3402 0.19 +1.6 3,529,088 - yhgE b3402 0.20 +0.7 3,529,135 - yhgE b3402 0.23 +0.2 3,529,135 - yhgE b3402 0.23 -0.2 3,529,138 - yhgE b3402 0.23 +1.1 3,529,173 - yhgE b3402 0.25 -0.0 3,529,173 - yhgE b3402 0.25 +0.2 3,529,206 + yhgE b3402 0.27 +1.0 3,529,228 - yhgE b3402 0.28 +1.1 3,529,252 + yhgE b3402 0.30 -0.5 3,529,252 + yhgE b3402 0.30 +0.7 3,529,260 - yhgE b3402 0.30 -0.4 3,529,260 - yhgE b3402 0.30 +0.6 3,529,261 + yhgE b3402 0.30 +0.3 3,529,261 + yhgE b3402 0.30 -0.9 3,529,267 + yhgE b3402 0.31 +0.4 3,529,317 - yhgE b3402 0.34 +0.9 3,529,338 - yhgE b3402 0.35 +0.2 3,529,338 - yhgE b3402 0.35 -0.4 3,529,340 + yhgE b3402 0.35 -0.9 3,529,340 + yhgE b3402 0.35 -0.3 3,529,348 - yhgE b3402 0.35 -0.8 3,529,348 - yhgE b3402 0.35 +0.1 3,529,377 - yhgE b3402 0.37 +0.2 3,529,377 - yhgE b3402 0.37 -0.6 3,529,377 - yhgE b3402 0.37 +0.5 3,529,377 - yhgE b3402 0.37 +0.1 3,529,437 + yhgE b3402 0.41 +0.4 3,529,443 - yhgE b3402 0.41 +1.1 3,529,457 - yhgE b3402 0.42 +3.0 3,529,470 + yhgE b3402 0.42 +0.2 3,529,511 + yhgE b3402 0.45 +0.2 3,529,511 + yhgE b3402 0.45 -0.1 3,529,532 - yhgE b3402 0.46 +0.2 3,529,534 - yhgE b3402 0.46 -0.5 3,529,537 - yhgE b3402 0.46 +0.3 3,529,537 - yhgE b3402 0.46 +0.1 3,529,592 - yhgE b3402 0.50 -0.3 3,529,626 + yhgE b3402 0.52 -0.6 3,529,626 + yhgE b3402 0.52 -2.3 3,529,626 + yhgE b3402 0.52 -0.6 3,529,626 + yhgE b3402 0.52 -1.1 3,529,639 - yhgE b3402 0.52 +0.2
Or see this region's nucleotide sequence