Experiment: deuterated glucose in D2O
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tktB and ypfG are separated by 94 nucleotides ypfG and yffH are separated by 125 nucleotides yffH and aegA are separated by 67 nucleotides
b2465: tktB - transketolase 2, thiamin-binding (NCBI), at 2,577,658 to 2,579,661
tktB
b2466: ypfG - hypothetical protein (NCBI), at 2,579,756 to 2,580,799
ypfG
b2467: yffH - predicted NUDIX hydrolase (NCBI), at 2,580,925 to 2,581,500
yffH
b2468: aegA - fused predicted oxidoreductase: FeS binding subunit/NAD/FAD-binding subunit (NCBI), at 2,581,568 to 2,583,547
aegA
Position (kb)
2579
2580
2581 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2578.865 kb on + strand, within tktB at 2579.050 kb on - strand, within tktB at 2579.050 kb on - strand, within tktB at 2579.050 kb on - strand, within tktB at 2579.050 kb on - strand, within tktB at 2579.079 kb on - strand, within tktB at 2579.181 kb on + strand, within tktB at 2579.205 kb on - strand, within tktB at 2579.369 kb on + strand, within tktB at 2579.406 kb on - strand, within tktB at 2579.406 kb on - strand, within tktB at 2579.438 kb on - strand, within tktB at 2579.438 kb on - strand, within tktB at 2579.586 kb on + strand at 2579.702 kb on - strand at 2579.799 kb on - strand at 2579.799 kb on - strand at 2579.825 kb on + strand at 2579.825 kb on + strand at 2580.163 kb on - strand, within ypfG at 2580.219 kb on + strand, within ypfG at 2580.222 kb on - strand, within ypfG at 2580.222 kb on - strand, within ypfG at 2580.223 kb on - strand, within ypfG at 2580.258 kb on - strand, within ypfG at 2580.264 kb on - strand, within ypfG at 2580.517 kb on + strand, within ypfG at 2580.572 kb on + strand, within ypfG at 2580.609 kb on + strand, within ypfG at 2580.609 kb on + strand, within ypfG at 2580.959 kb on + strand at 2580.959 kb on + strand at 2581.059 kb on - strand, within yffH at 2581.099 kb on - strand, within yffH at 2581.124 kb on + strand, within yffH at 2581.325 kb on + strand, within yffH at 2581.335 kb on - strand, within yffH at 2581.342 kb on + strand, within yffH at 2581.350 kb on - strand, within yffH at 2581.369 kb on - strand at 2581.372 kb on + strand, within yffH at 2581.380 kb on - strand, within yffH at 2581.380 kb on - strand, within yffH at 2581.445 kb on + strand at 2581.453 kb on - strand at 2581.453 kb on - strand at 2581.570 kb on + strand at 2581.570 kb on + strand at 2581.662 kb on - strand at 2581.712 kb on - strand at 2581.718 kb on - strand at 2581.718 kb on - strand at 2581.759 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction deuterated glucose in D2O remove 2,578,865 + tktB b2465 0.60 -0.0 2,579,050 - tktB b2465 0.69 +0.5 2,579,050 - tktB b2465 0.69 -0.5 2,579,050 - tktB b2465 0.69 +0.5 2,579,050 - tktB b2465 0.69 -0.7 2,579,079 - tktB b2465 0.71 +0.3 2,579,181 + tktB b2465 0.76 -1.3 2,579,205 - tktB b2465 0.77 +1.3 2,579,369 + tktB b2465 0.85 +1.1 2,579,406 - tktB b2465 0.87 -0.0 2,579,406 - tktB b2465 0.87 +0.6 2,579,438 - tktB b2465 0.89 +0.1 2,579,438 - tktB b2465 0.89 +0.0 2,579,586 + +0.5 2,579,702 - -0.3 2,579,799 - +1.5 2,579,799 - +1.3 2,579,825 + -0.0 2,579,825 + +0.2 2,580,163 - ypfG b2466 0.39 -1.7 2,580,219 + ypfG b2466 0.44 -0.8 2,580,222 - ypfG b2466 0.45 +0.4 2,580,222 - ypfG b2466 0.45 +4.4 2,580,223 - ypfG b2466 0.45 -0.2 2,580,258 - ypfG b2466 0.48 -0.1 2,580,264 - ypfG b2466 0.49 +1.0 2,580,517 + ypfG b2466 0.73 +0.3 2,580,572 + ypfG b2466 0.78 +0.1 2,580,609 + ypfG b2466 0.82 +0.3 2,580,609 + ypfG b2466 0.82 +1.1 2,580,959 + +0.0 2,580,959 + -0.9 2,581,059 - yffH b2467 0.23 +0.1 2,581,099 - yffH b2467 0.30 -0.7 2,581,124 + yffH b2467 0.35 +0.5 2,581,325 + yffH b2467 0.69 -0.4 2,581,335 - yffH b2467 0.71 +0.6 2,581,342 + yffH b2467 0.72 -0.6 2,581,350 - yffH b2467 0.74 +0.3 2,581,369 - +1.3 2,581,372 + yffH b2467 0.78 -0.4 2,581,380 - yffH b2467 0.79 +0.6 2,581,380 - yffH b2467 0.79 +0.3 2,581,445 + -0.2 2,581,453 - -1.0 2,581,453 - -0.9 2,581,570 + +0.6 2,581,570 + +0.9 2,581,662 - +0.7 2,581,712 - +1.0 2,581,718 - +2.4 2,581,718 - +0.4 2,581,759 + -0.0
Or see this region's nucleotide sequence