Strain Fitness in Escherichia coli BW25113 around b2102

Experiment: deuterated glucose in D2O

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyegV and yegW overlap by 27 nucleotidesyegW and yegX are separated by 51 nucleotidesyegX and thiD are separated by 64 nucleotidesthiD and thiM overlap by 4 nucleotides b2100: yegV - predicted kinase (NCBI), at 2,179,118 to 2,180,083 yegV b2101: yegW - predicted DNA-binding transcriptional regulator (NCBI), at 2,180,057 to 2,180,803 yegW b2102: yegX - orf, hypothetical protein (VIMSS), at 2,180,855 to 2,181,673 yegX b2103: thiD - phosphomethylpyrimidine kinase (NCBI), at 2,181,738 to 2,182,538 thiD b2104: thiM - hydroxyethylthiazole kinase (NCBI), at 2,182,535 to 2,183,323 thiM Position (kb) 2180 2181 2182Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2179.906 kb on - strand, within yegVat 2179.906 kb on - strand, within yegVat 2179.993 kb on - strandat 2180.103 kb on + strandat 2180.234 kb on + strand, within yegWat 2180.502 kb on - strand, within yegWat 2180.515 kb on + strand, within yegWat 2180.595 kb on - strand, within yegWat 2180.604 kb on + strand, within yegWat 2180.604 kb on + strand, within yegWat 2180.686 kb on - strand, within yegWat 2180.686 kb on - strand, within yegWat 2180.686 kb on - strand, within yegWat 2180.829 kb on + strandat 2180.837 kb on - strandat 2180.886 kb on + strandat 2180.896 kb on - strandat 2180.902 kb on - strandat 2180.902 kb on - strandat 2180.904 kb on - strandat 2180.917 kb on - strandat 2180.934 kb on + strandat 2180.934 kb on + strandat 2180.961 kb on - strand, within yegXat 2181.005 kb on - strandat 2181.005 kb on - strand, within yegXat 2181.027 kb on + strand, within yegXat 2181.027 kb on + strand, within yegXat 2181.202 kb on - strand, within yegXat 2181.202 kb on - strand, within yegXat 2181.221 kb on + strand, within yegXat 2181.256 kb on - strand, within yegXat 2181.376 kb on + strand, within yegXat 2181.460 kb on + strand, within yegXat 2181.467 kb on + strand, within yegXat 2181.485 kb on - strand, within yegXat 2181.497 kb on - strand, within yegXat 2181.516 kb on - strand, within yegXat 2181.525 kb on + strand, within yegXat 2181.548 kb on + strand, within yegXat 2181.628 kb on + strandat 2181.628 kb on + strandat 2181.631 kb on + strandat 2181.660 kb on + strandat 2181.678 kb on - strandat 2181.695 kb on + strandat 2181.726 kb on + strandat 2181.731 kb on - strandat 2181.749 kb on - strandat 2181.801 kb on - strandat 2181.806 kb on - strandat 2181.875 kb on + strand, within thiDat 2181.883 kb on - strand, within thiDat 2181.883 kb on - strand, within thiDat 2181.883 kb on - strand, within thiDat 2182.009 kb on + strand, within thiDat 2182.009 kb on + strand, within thiDat 2182.012 kb on + strand, within thiDat 2182.020 kb on - strand, within thiDat 2182.119 kb on - strand, within thiDat 2182.150 kb on - strand, within thiDat 2182.161 kb on + strand, within thiDat 2182.375 kb on + strand, within thiDat 2182.375 kb on + strand, within thiDat 2182.382 kb on + strand, within thiDat 2182.546 kb on - strandat 2182.646 kb on + strand, within thiM

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Per-strain Table

Position Strand Gene LocusTag Fraction deuterated glucose in D2O
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2,179,906 - yegV b2100 0.82 +0.3
2,179,906 - yegV b2100 0.82 +1.0
2,179,993 - +0.3
2,180,103 + -1.6
2,180,234 + yegW b2101 0.24 -0.1
2,180,502 - yegW b2101 0.60 -0.0
2,180,515 + yegW b2101 0.61 +0.1
2,180,595 - yegW b2101 0.72 +0.5
2,180,604 + yegW b2101 0.73 +0.9
2,180,604 + yegW b2101 0.73 +1.4
2,180,686 - yegW b2101 0.84 +0.3
2,180,686 - yegW b2101 0.84 +1.0
2,180,686 - yegW b2101 0.84 -0.2
2,180,829 + -0.8
2,180,837 - -0.5
2,180,886 + +0.1
2,180,896 - +0.5
2,180,902 - -0.0
2,180,902 - +0.4
2,180,904 - +0.1
2,180,917 - +0.2
2,180,934 + +2.0
2,180,934 + +0.4
2,180,961 - yegX b2102 0.13 -0.0
2,181,005 - +0.6
2,181,005 - yegX b2102 0.18 -0.1
2,181,027 + yegX b2102 0.21 -0.8
2,181,027 + yegX b2102 0.21 +1.4
2,181,202 - yegX b2102 0.42 +0.2
2,181,202 - yegX b2102 0.42 +0.6
2,181,221 + yegX b2102 0.45 -0.1
2,181,256 - yegX b2102 0.49 +0.6
2,181,376 + yegX b2102 0.64 +0.4
2,181,460 + yegX b2102 0.74 +2.1
2,181,467 + yegX b2102 0.75 +2.1
2,181,485 - yegX b2102 0.77 +0.3
2,181,497 - yegX b2102 0.78 -0.9
2,181,516 - yegX b2102 0.81 +1.1
2,181,525 + yegX b2102 0.82 -0.4
2,181,548 + yegX b2102 0.85 -0.1
2,181,628 + -0.3
2,181,628 + +0.7
2,181,631 + +0.1
2,181,660 + +0.1
2,181,678 - +0.4
2,181,695 + -0.1
2,181,726 + +0.1
2,181,731 - -0.4
2,181,749 - -3.0
2,181,801 - -3.3
2,181,806 - +0.4
2,181,875 + thiD b2103 0.17 -3.0
2,181,883 - thiD b2103 0.18 -3.2
2,181,883 - thiD b2103 0.18 -2.0
2,181,883 - thiD b2103 0.18 -2.5
2,182,009 + thiD b2103 0.34 -1.3
2,182,009 + thiD b2103 0.34 +0.8
2,182,012 + thiD b2103 0.34 -2.1
2,182,020 - thiD b2103 0.35 -1.6
2,182,119 - thiD b2103 0.48 -1.6
2,182,150 - thiD b2103 0.51 -2.1
2,182,161 + thiD b2103 0.53 -1.0
2,182,375 + thiD b2103 0.80 -2.3
2,182,375 + thiD b2103 0.80 -0.6
2,182,382 + thiD b2103 0.80 -3.5
2,182,546 - +0.3
2,182,646 + thiM b2104 0.14 -1.6

Or see this region's nucleotide sequence