Experiment: deuterated glucose in D2O
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rsxA and rsxB overlap by 1 nucleotides rsxB and rsxC overlap by 8 nucleotides rsxC and rsxD are separated by 0 nucleotides
b1627: rsxA - Na(+)-translocating NADH-quinone reductase subunit E (NCBI), at 1,703,791 to 1,704,372
rsxA
b1628: rsxB - electron transport complex protein RnfB (NCBI), at 1,704,372 to 1,704,950
rsxB
b1629: rsxC - electron transport complex protein RnfC (NCBI), at 1,704,943 to 1,707,165
rsxC
b1630: rsxD - electron transport complex protein RnfD (NCBI), at 1,707,166 to 1,708,224
rsxD
Position (kb)
1704
1705
1706
1707
1708 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1704.048 kb on + strand, within rsxA at 1704.090 kb on - strand, within rsxA at 1704.235 kb on - strand, within rsxA at 1704.376 kb on + strand at 1704.376 kb on + strand at 1704.377 kb on - strand at 1704.525 kb on - strand, within rsxB at 1704.772 kb on + strand, within rsxB at 1704.806 kb on + strand, within rsxB at 1705.039 kb on + strand at 1705.042 kb on - strand at 1705.235 kb on - strand, within rsxC at 1705.290 kb on + strand, within rsxC at 1705.290 kb on + strand, within rsxC at 1705.308 kb on - strand, within rsxC at 1705.308 kb on - strand, within rsxC at 1705.459 kb on + strand, within rsxC at 1705.459 kb on + strand, within rsxC at 1705.529 kb on - strand, within rsxC at 1705.579 kb on + strand, within rsxC at 1705.727 kb on - strand, within rsxC at 1705.812 kb on + strand, within rsxC at 1705.999 kb on + strand, within rsxC at 1706.363 kb on + strand, within rsxC at 1706.547 kb on - strand, within rsxC at 1706.829 kb on + strand, within rsxC at 1706.859 kb on + strand, within rsxC at 1706.859 kb on + strand, within rsxC at 1706.883 kb on - strand, within rsxC at 1706.883 kb on - strand, within rsxC at 1706.924 kb on + strand, within rsxC at 1707.252 kb on + strand at 1707.252 kb on + strand at 1707.328 kb on + strand, within rsxD at 1707.414 kb on + strand, within rsxD at 1707.414 kb on + strand, within rsxD at 1707.473 kb on - strand, within rsxD at 1707.579 kb on - strand, within rsxD at 1707.696 kb on - strand, within rsxD at 1707.716 kb on + strand, within rsxD at 1707.793 kb on + strand, within rsxD at 1707.803 kb on - strand, within rsxD at 1707.895 kb on + strand, within rsxD at 1707.914 kb on - strand, within rsxD at 1707.924 kb on + strand, within rsxD at 1708.021 kb on + strand, within rsxD at 1708.161 kb on + strand at 1708.161 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction deuterated glucose in D2O remove 1,704,048 + rsxA b1627 0.44 -2.3 1,704,090 - rsxA b1627 0.51 -2.0 1,704,235 - rsxA b1627 0.76 +0.2 1,704,376 + -0.1 1,704,376 + -2.3 1,704,377 - -0.7 1,704,525 - rsxB b1628 0.26 -1.2 1,704,772 + rsxB b1628 0.69 -2.0 1,704,806 + rsxB b1628 0.75 -1.7 1,705,039 + -2.2 1,705,042 - -0.1 1,705,235 - rsxC b1629 0.13 -1.5 1,705,290 + rsxC b1629 0.16 -0.8 1,705,290 + rsxC b1629 0.16 -3.2 1,705,308 - rsxC b1629 0.16 +0.2 1,705,308 - rsxC b1629 0.16 -1.0 1,705,459 + rsxC b1629 0.23 -1.8 1,705,459 + rsxC b1629 0.23 -1.1 1,705,529 - rsxC b1629 0.26 -2.5 1,705,579 + rsxC b1629 0.29 -3.2 1,705,727 - rsxC b1629 0.35 -2.8 1,705,812 + rsxC b1629 0.39 -1.8 1,705,999 + rsxC b1629 0.48 -2.2 1,706,363 + rsxC b1629 0.64 -0.3 1,706,547 - rsxC b1629 0.72 -1.5 1,706,829 + rsxC b1629 0.85 -1.5 1,706,859 + rsxC b1629 0.86 +0.4 1,706,859 + rsxC b1629 0.86 -0.9 1,706,883 - rsxC b1629 0.87 -0.6 1,706,883 - rsxC b1629 0.87 -0.8 1,706,924 + rsxC b1629 0.89 -0.8 1,707,252 + -0.3 1,707,252 + -0.6 1,707,328 + rsxD b1630 0.15 +0.8 1,707,414 + rsxD b1630 0.23 -1.1 1,707,414 + rsxD b1630 0.23 -1.4 1,707,473 - rsxD b1630 0.29 -1.0 1,707,579 - rsxD b1630 0.39 -1.3 1,707,696 - rsxD b1630 0.50 -1.1 1,707,716 + rsxD b1630 0.52 -0.9 1,707,793 + rsxD b1630 0.59 -2.0 1,707,803 - rsxD b1630 0.60 -1.2 1,707,895 + rsxD b1630 0.69 -2.8 1,707,914 - rsxD b1630 0.71 -1.6 1,707,924 + rsxD b1630 0.72 -1.2 1,708,021 + rsxD b1630 0.81 -2.4 1,708,161 + -2.0 1,708,161 + -1.5
Or see this region's nucleotide sequence