Experiment: deuterated glucose in D2O
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybbB and ybbS are separated by 68 nucleotides ybbS and allA are separated by 229 nucleotides allA and allR are separated by 77 nucleotides
b0503: ybbB - tRNA 2-selenouridine synthase, selenophosphate-dependent (NCBI), at 529,356 to 530,450
ybbB
b0504: ybbS - DNA-binding transcriptional activator of the allD operon (NCBI), at 530,519 to 531,445
ybbS
b0505: allA - ureidoglycolate hydrolase (NCBI), at 531,675 to 532,157
allA
b0506: allR - DNA-binding transcriptional repressor (NCBI), at 532,235 to 533,050
allR
Position (kb) 530
531
532 Strain fitness (log2 ratio) -1
0
1
2
3 at 529.728 kb on - strand, within ybbB at 529.728 kb on - strand, within ybbB at 529.906 kb on - strand, within ybbB at 529.911 kb on + strand, within ybbB at 529.911 kb on + strand, within ybbB at 529.959 kb on + strand, within ybbB at 529.959 kb on + strand, within ybbB at 530.005 kb on - strand, within ybbB at 530.209 kb on + strand, within ybbB at 530.583 kb on - strand at 530.583 kb on - strand at 531.058 kb on - strand, within ybbS at 531.077 kb on - strand, within ybbS at 531.113 kb on + strand, within ybbS at 531.134 kb on - strand, within ybbS at 531.229 kb on + strand, within ybbS at 531.687 kb on + strand at 531.687 kb on + strand at 531.776 kb on + strand, within allA at 531.816 kb on + strand, within allA at 531.907 kb on - strand, within allA at 531.962 kb on - strand, within allA at 532.199 kb on + strand at 532.216 kb on - strand at 532.221 kb on + strand at 532.278 kb on + strand at 532.278 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction deuterated glucose in D2O remove 529,728 - ybbB b0503 0.34 +0.1 529,728 - ybbB b0503 0.34 -0.1 529,906 - ybbB b0503 0.50 +0.2 529,911 + ybbB b0503 0.51 +1.2 529,911 + ybbB b0503 0.51 +1.2 529,959 + ybbB b0503 0.55 +1.1 529,959 + ybbB b0503 0.55 +0.0 530,005 - ybbB b0503 0.59 +3.4 530,209 + ybbB b0503 0.78 +0.6 530,583 - +0.5 530,583 - +0.4 531,058 - ybbS b0504 0.58 +0.6 531,077 - ybbS b0504 0.60 +1.5 531,113 + ybbS b0504 0.64 +0.6 531,134 - ybbS b0504 0.66 +1.1 531,229 + ybbS b0504 0.77 +0.8 531,687 + +0.2 531,687 + +1.1 531,776 + allA b0505 0.21 +0.5 531,816 + allA b0505 0.29 +3.2 531,907 - allA b0505 0.48 -0.1 531,962 - allA b0505 0.59 +0.4 532,199 + +0.2 532,216 - +0.5 532,221 + +1.1 532,278 + -0.9 532,278 + +0.1
Or see this region's nucleotide sequence