Strain Fitness in Escherichia coli BW25113 around b0124

Experiment: deuterated glucose in D2O

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcueO and gcd are separated by 201 nucleotidesgcd and hpt are separated by 205 nucleotideshpt and can are separated by 40 nucleotides b0123: cueO - multicopper oxidase (laccase) (NCBI), at 137,083 to 138,633 cueO b0124: gcd - glucose dehydrogenase (NCBI), at 138,835 to 141,225 gcd b0125: hpt - hypoxanthine phosphoribosyltransferase (VIMSS), at 141,431 to 141,967 hpt b0126: can - carbonic anhydrase (NCBI), at 142,008 to 142,670 can Position (kb) 138 139 140 141 142Strain fitness (log2 ratio) -2 -1 0 1 2 3at 137.852 kb on - strand, within cueOat 137.889 kb on + strand, within cueOat 137.897 kb on + strand, within cueOat 137.907 kb on + strand, within cueOat 137.913 kb on - strand, within cueOat 138.067 kb on + strand, within cueOat 138.067 kb on + strand, within cueOat 138.070 kb on + strand, within cueOat 138.070 kb on + strand, within cueOat 138.232 kb on + strand, within cueOat 138.242 kb on + strand, within cueOat 138.252 kb on + strand, within cueOat 138.255 kb on - strand, within cueOat 138.352 kb on - strand, within cueOat 138.418 kb on + strand, within cueOat 138.418 kb on + strand, within cueOat 138.435 kb on - strand, within cueOat 138.435 kb on - strand, within cueOat 138.566 kb on + strandat 138.566 kb on + strandat 138.572 kb on - strandat 138.589 kb on - strandat 138.689 kb on - strandat 138.797 kb on - strandat 138.862 kb on + strandat 138.862 kb on + strandat 138.870 kb on - strandat 138.870 kb on - strandat 138.870 kb on - strandat 138.870 kb on - strandat 138.919 kb on - strandat 138.928 kb on + strandat 138.928 kb on + strandat 138.950 kb on - strandat 139.025 kb on + strandat 139.025 kb on + strandat 139.057 kb on - strandat 139.095 kb on - strand, within gcdat 139.095 kb on - strand, within gcdat 139.147 kb on + strand, within gcdat 139.203 kb on + strand, within gcdat 139.211 kb on + strand, within gcdat 139.211 kb on + strand, within gcdat 139.243 kb on + strand, within gcdat 139.245 kb on - strand, within gcdat 139.245 kb on - strand, within gcdat 139.267 kb on + strand, within gcdat 139.274 kb on + strand, within gcdat 139.282 kb on - strand, within gcdat 139.282 kb on - strand, within gcdat 139.284 kb on + strand, within gcdat 139.284 kb on + strand, within gcdat 139.285 kb on - strand, within gcdat 139.285 kb on - strand, within gcdat 139.288 kb on + strand, within gcdat 139.352 kb on - strand, within gcdat 139.405 kb on - strand, within gcdat 139.405 kb on - strand, within gcdat 139.469 kb on - strand, within gcdat 139.469 kb on - strand, within gcdat 139.492 kb on - strand, within gcdat 139.492 kb on - strand, within gcdat 139.552 kb on + strand, within gcdat 139.623 kb on + strand, within gcdat 139.623 kb on + strand, within gcdat 139.670 kb on - strand, within gcdat 139.795 kb on - strand, within gcdat 139.795 kb on - strand, within gcdat 139.836 kb on + strand, within gcdat 139.836 kb on + strand, within gcdat 139.861 kb on - strand, within gcdat 140.007 kb on - strand, within gcdat 140.007 kb on - strand, within gcdat 140.020 kb on + strand, within gcdat 140.161 kb on - strand, within gcdat 140.466 kb on + strand, within gcdat 140.541 kb on + strand, within gcdat 140.542 kb on - strand, within gcdat 140.542 kb on - strand, within gcdat 140.576 kb on - strand, within gcdat 140.580 kb on - strand, within gcdat 140.630 kb on + strand, within gcdat 140.634 kb on - strand, within gcdat 140.663 kb on + strand, within gcdat 140.710 kb on + strand, within gcdat 140.710 kb on + strand, within gcdat 140.733 kb on - strand, within gcdat 140.733 kb on - strand, within gcdat 140.744 kb on - strand, within gcdat 140.744 kb on - strand, within gcdat 140.818 kb on + strand, within gcdat 140.818 kb on + strand, within gcdat 140.826 kb on - strand, within gcdat 140.826 kb on - strand, within gcdat 140.842 kb on - strand, within gcdat 140.854 kb on - strand, within gcdat 140.854 kb on - strand, within gcdat 140.856 kb on + strand, within gcdat 141.007 kb on + strandat 141.010 kb on + strandat 141.059 kb on + strandat 141.067 kb on - strandat 141.078 kb on + strandat 141.086 kb on - strandat 141.095 kb on + strandat 141.103 kb on - strandat 141.103 kb on - strandat 141.134 kb on + strandat 141.134 kb on + strandat 141.182 kb on - strandat 141.229 kb on - strandat 141.365 kb on + strandat 141.513 kb on - strand, within hptat 141.566 kb on + strand, within hptat 141.566 kb on + strand, within hptat 141.574 kb on - strand, within hptat 141.574 kb on - strand, within hptat 141.586 kb on + strand, within hptat 141.588 kb on + strand, within hptat 141.588 kb on + strand, within hptat 141.591 kb on - strand, within hptat 141.591 kb on - strand, within hptat 141.618 kb on + strand, within hptat 141.899 kb on + strand, within hptat 141.906 kb on + strand, within hptat 141.910 kb on + strand, within hptat 141.910 kb on + strand, within hptat 141.967 kb on + strandat 142.000 kb on - strandat 142.000 kb on - strandat 142.184 kb on - strand, within can

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Per-strain Table

Position Strand Gene LocusTag Fraction deuterated glucose in D2O
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137,852 - cueO b0123 0.50 +0.2
137,889 + cueO b0123 0.52 -0.0
137,897 + cueO b0123 0.52 +0.4
137,907 + cueO b0123 0.53 +1.6
137,913 - cueO b0123 0.54 -0.0
138,067 + cueO b0123 0.63 +0.8
138,067 + cueO b0123 0.63 +0.8
138,070 + cueO b0123 0.64 -0.1
138,070 + cueO b0123 0.64 +0.2
138,232 + cueO b0123 0.74 +0.4
138,242 + cueO b0123 0.75 +0.0
138,252 + cueO b0123 0.75 +0.1
138,255 - cueO b0123 0.76 +0.5
138,352 - cueO b0123 0.82 +2.8
138,418 + cueO b0123 0.86 -1.6
138,418 + cueO b0123 0.86 +1.5
138,435 - cueO b0123 0.87 +0.7
138,435 - cueO b0123 0.87 -0.6
138,566 + +0.0
138,566 + -0.1
138,572 - +0.6
138,589 - -0.5
138,689 - +0.2
138,797 - +0.0
138,862 + +0.2
138,862 + +0.7
138,870 - +0.9
138,870 - -0.0
138,870 - +0.3
138,870 - -0.3
138,919 - -1.0
138,928 + -0.8
138,928 + -0.6
138,950 - +0.6
139,025 + -0.5
139,025 + +1.2
139,057 - +0.7
139,095 - gcd b0124 0.11 +0.6
139,095 - gcd b0124 0.11 -0.2
139,147 + gcd b0124 0.13 -0.3
139,203 + gcd b0124 0.15 -0.2
139,211 + gcd b0124 0.16 -0.2
139,211 + gcd b0124 0.16 -0.7
139,243 + gcd b0124 0.17 +0.2
139,245 - gcd b0124 0.17 -0.4
139,245 - gcd b0124 0.17 -0.9
139,267 + gcd b0124 0.18 +0.7
139,274 + gcd b0124 0.18 -0.1
139,282 - gcd b0124 0.19 +0.2
139,282 - gcd b0124 0.19 +0.4
139,284 + gcd b0124 0.19 -0.6
139,284 + gcd b0124 0.19 +0.2
139,285 - gcd b0124 0.19 +0.8
139,285 - gcd b0124 0.19 +0.6
139,288 + gcd b0124 0.19 +0.2
139,352 - gcd b0124 0.22 +0.6
139,405 - gcd b0124 0.24 -0.8
139,405 - gcd b0124 0.24 -0.1
139,469 - gcd b0124 0.27 +0.3
139,469 - gcd b0124 0.27 +2.8
139,492 - gcd b0124 0.27 +0.1
139,492 - gcd b0124 0.27 -0.3
139,552 + gcd b0124 0.30 +0.7
139,623 + gcd b0124 0.33 +0.5
139,623 + gcd b0124 0.33 +1.2
139,670 - gcd b0124 0.35 +1.2
139,795 - gcd b0124 0.40 +0.4
139,795 - gcd b0124 0.40 +0.5
139,836 + gcd b0124 0.42 -0.8
139,836 + gcd b0124 0.42 -0.7
139,861 - gcd b0124 0.43 -0.1
140,007 - gcd b0124 0.49 +0.7
140,007 - gcd b0124 0.49 +1.7
140,020 + gcd b0124 0.50 -0.4
140,161 - gcd b0124 0.55 -0.0
140,466 + gcd b0124 0.68 -0.3
140,541 + gcd b0124 0.71 +0.7
140,542 - gcd b0124 0.71 -0.1
140,542 - gcd b0124 0.71 -0.6
140,576 - gcd b0124 0.73 +0.2
140,580 - gcd b0124 0.73 +0.4
140,630 + gcd b0124 0.75 -0.2
140,634 - gcd b0124 0.75 -0.6
140,663 + gcd b0124 0.76 +0.4
140,710 + gcd b0124 0.78 +1.2
140,710 + gcd b0124 0.78 +0.2
140,733 - gcd b0124 0.79 -0.2
140,733 - gcd b0124 0.79 -0.5
140,744 - gcd b0124 0.80 -0.2
140,744 - gcd b0124 0.80 +0.2
140,818 + gcd b0124 0.83 +0.8
140,818 + gcd b0124 0.83 +1.3
140,826 - gcd b0124 0.83 +1.8
140,826 - gcd b0124 0.83 +1.7
140,842 - gcd b0124 0.84 +0.3
140,854 - gcd b0124 0.84 +1.2
140,854 - gcd b0124 0.84 -0.2
140,856 + gcd b0124 0.85 -2.1
141,007 + -0.2
141,010 + -0.5
141,059 + -0.5
141,067 - -0.8
141,078 + +0.2
141,086 - -0.6
141,095 + -1.3
141,103 - +0.8
141,103 - +1.2
141,134 + +0.7
141,134 + +1.2
141,182 - -0.2
141,229 - +0.0
141,365 + +0.6
141,513 - hpt b0125 0.15 +1.2
141,566 + hpt b0125 0.25 -0.2
141,566 + hpt b0125 0.25 +1.2
141,574 - hpt b0125 0.27 +0.6
141,574 - hpt b0125 0.27 +0.6
141,586 + hpt b0125 0.29 +0.0
141,588 + hpt b0125 0.29 +2.0
141,588 + hpt b0125 0.29 -0.2
141,591 - hpt b0125 0.30 +0.9
141,591 - hpt b0125 0.30 -0.1
141,618 + hpt b0125 0.35 -0.1
141,899 + hpt b0125 0.87 +0.3
141,906 + hpt b0125 0.88 +1.8
141,910 + hpt b0125 0.89 +0.3
141,910 + hpt b0125 0.89 +0.9
141,967 + -0.6
142,000 - +0.2
142,000 - -0.3
142,184 - can b0126 0.27 +2.1

Or see this region's nucleotide sequence