Experiment: deuterated glucose in D2O
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ampD and ampE overlap by 4 nucleotides ampE and aroP are separated by 42 nucleotides aroP and pdhR are separated by 540 nucleotides
b0110: ampD - N-acetyl-anhydromuranmyl-L-alanine amidase (NCBI), at 118,733 to 119,284
ampD
b0111: ampE - predicted inner membrane protein (NCBI), at 119,281 to 120,135
ampE
b0112: aroP - aromatic amino acid transporter (NCBI), at 120,178 to 121,551
aroP
b0113: pdhR - transcriptional regulator of pyruvate dehydrogenase complex (NCBI), at 122,092 to 122,856
pdhR
Position (kb)
120
121
122 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 119.191 kb on + strand, within ampD at 119.191 kb on + strand, within ampD at 119.274 kb on + strand at 119.339 kb on + strand at 119.339 kb on + strand at 119.499 kb on - strand, within ampE at 119.499 kb on - strand, within ampE at 119.522 kb on + strand, within ampE at 119.528 kb on - strand, within ampE at 119.555 kb on - strand, within ampE at 119.555 kb on - strand, within ampE at 119.558 kb on - strand, within ampE at 119.567 kb on + strand, within ampE at 119.567 kb on + strand, within ampE at 119.753 kb on - strand, within ampE at 119.755 kb on - strand, within ampE at 119.755 kb on - strand, within ampE at 119.756 kb on + strand, within ampE at 119.772 kb on - strand, within ampE at 119.786 kb on + strand, within ampE at 119.786 kb on + strand, within ampE at 119.799 kb on - strand, within ampE at 119.799 kb on - strand, within ampE at 119.836 kb on - strand, within ampE at 119.836 kb on - strand, within ampE at 119.915 kb on + strand, within ampE at 119.961 kb on + strand, within ampE at 119.961 kb on + strand, within ampE at 119.983 kb on + strand, within ampE at 119.985 kb on - strand, within ampE at 120.006 kb on - strand, within ampE at 120.013 kb on - strand, within ampE at 120.013 kb on - strand, within ampE at 120.014 kb on + strand, within ampE at 120.014 kb on + strand, within ampE at 120.014 kb on + strand, within ampE at 120.014 kb on + strand, within ampE at 120.037 kb on - strand, within ampE at 120.138 kb on - strand at 120.139 kb on + strand at 120.153 kb on - strand at 120.155 kb on + strand at 120.237 kb on - strand at 120.251 kb on - strand at 120.254 kb on + strand at 120.266 kb on - strand at 120.266 kb on - strand at 120.340 kb on - strand, within aroP at 120.362 kb on - strand, within aroP at 120.377 kb on + strand, within aroP at 120.389 kb on - strand, within aroP at 120.433 kb on + strand, within aroP at 120.461 kb on + strand, within aroP at 120.484 kb on + strand, within aroP at 120.502 kb on - strand, within aroP at 120.502 kb on - strand, within aroP at 120.514 kb on - strand, within aroP at 120.514 kb on - strand, within aroP at 120.518 kb on + strand, within aroP at 120.560 kb on - strand, within aroP at 120.571 kb on - strand, within aroP at 120.611 kb on - strand, within aroP at 120.611 kb on - strand, within aroP at 120.647 kb on - strand, within aroP at 120.647 kb on - strand, within aroP at 120.675 kb on + strand, within aroP at 120.683 kb on - strand, within aroP at 120.706 kb on + strand, within aroP at 120.712 kb on + strand, within aroP at 120.712 kb on + strand, within aroP at 120.752 kb on + strand, within aroP at 120.753 kb on - strand, within aroP at 120.753 kb on - strand, within aroP at 120.847 kb on + strand, within aroP at 120.854 kb on - strand, within aroP at 120.905 kb on - strand, within aroP at 120.905 kb on - strand, within aroP at 120.956 kb on - strand, within aroP at 120.956 kb on - strand, within aroP at 121.032 kb on - strand, within aroP at 121.032 kb on - strand, within aroP at 121.032 kb on - strand, within aroP at 121.054 kb on + strand, within aroP at 121.087 kb on + strand, within aroP at 121.095 kb on - strand, within aroP at 121.128 kb on - strand, within aroP at 121.128 kb on - strand, within aroP at 121.146 kb on - strand, within aroP at 121.146 kb on - strand, within aroP at 121.266 kb on - strand, within aroP at 121.266 kb on - strand, within aroP at 121.335 kb on - strand, within aroP at 121.374 kb on - strand, within aroP at 121.374 kb on - strand, within aroP at 121.431 kb on + strand at 121.431 kb on + strand at 121.458 kb on - strand at 121.469 kb on - strand at 121.514 kb on + strand at 121.531 kb on - strand at 121.716 kb on - strand at 121.716 kb on - strand at 121.723 kb on - strand at 121.723 kb on - strand at 121.738 kb on - strand at 122.355 kb on + strand, within pdhR at 122.358 kb on + strand, within pdhR at 122.369 kb on + strand, within pdhR at 122.416 kb on + strand, within pdhR at 122.442 kb on + strand, within pdhR at 122.471 kb on + strand, within pdhR at 122.489 kb on + strand, within pdhR at 122.489 kb on + strand, within pdhR at 122.489 kb on + strand, within pdhR at 122.500 kb on + strand, within pdhR at 122.537 kb on + strand, within pdhR
Per-strain Table
Position Strand Gene LocusTag Fraction deuterated glucose in D2O remove 119,191 + ampD b0110 0.83 -0.1 119,191 + ampD b0110 0.83 +0.6 119,274 + -0.1 119,339 + +0.6 119,339 + +1.0 119,499 - ampE b0111 0.25 -4.8 119,499 - ampE b0111 0.25 -0.0 119,522 + ampE b0111 0.28 -0.3 119,528 - ampE b0111 0.29 -0.8 119,555 - ampE b0111 0.32 +0.6 119,555 - ampE b0111 0.32 -0.1 119,558 - ampE b0111 0.32 -0.5 119,567 + ampE b0111 0.33 -0.7 119,567 + ampE b0111 0.33 +0.7 119,753 - ampE b0111 0.55 +0.3 119,755 - ampE b0111 0.55 +0.5 119,755 - ampE b0111 0.55 -0.0 119,756 + ampE b0111 0.56 -0.1 119,772 - ampE b0111 0.57 +0.7 119,786 + ampE b0111 0.59 -0.2 119,786 + ampE b0111 0.59 +0.1 119,799 - ampE b0111 0.61 +0.0 119,799 - ampE b0111 0.61 +0.1 119,836 - ampE b0111 0.65 +0.6 119,836 - ampE b0111 0.65 -0.6 119,915 + ampE b0111 0.74 +1.0 119,961 + ampE b0111 0.80 +0.5 119,961 + ampE b0111 0.80 +1.5 119,983 + ampE b0111 0.82 +0.5 119,985 - ampE b0111 0.82 -0.7 120,006 - ampE b0111 0.85 -0.0 120,013 - ampE b0111 0.86 +0.0 120,013 - ampE b0111 0.86 +1.3 120,014 + ampE b0111 0.86 +1.8 120,014 + ampE b0111 0.86 -0.0 120,014 + ampE b0111 0.86 +0.2 120,014 + ampE b0111 0.86 -1.4 120,037 - ampE b0111 0.88 +0.7 120,138 - -0.7 120,139 + +0.3 120,153 - +0.3 120,155 + +1.0 120,237 - +0.5 120,251 - -0.8 120,254 + -2.4 120,266 - -0.4 120,266 - -2.1 120,340 - aroP b0112 0.12 -0.1 120,362 - aroP b0112 0.13 +0.8 120,377 + aroP b0112 0.14 -0.7 120,389 - aroP b0112 0.15 +2.1 120,433 + aroP b0112 0.19 +1.8 120,461 + aroP b0112 0.21 -1.3 120,484 + aroP b0112 0.22 -0.6 120,502 - aroP b0112 0.24 -0.9 120,502 - aroP b0112 0.24 -0.4 120,514 - aroP b0112 0.24 -0.8 120,514 - aroP b0112 0.24 -0.7 120,518 + aroP b0112 0.25 -0.8 120,560 - aroP b0112 0.28 -0.8 120,571 - aroP b0112 0.29 +0.8 120,611 - aroP b0112 0.32 +0.4 120,611 - aroP b0112 0.32 -1.5 120,647 - aroP b0112 0.34 +0.2 120,647 - aroP b0112 0.34 -0.1 120,675 + aroP b0112 0.36 -0.8 120,683 - aroP b0112 0.37 -1.2 120,706 + aroP b0112 0.38 -0.3 120,712 + aroP b0112 0.39 -1.9 120,712 + aroP b0112 0.39 -0.5 120,752 + aroP b0112 0.42 -2.4 120,753 - aroP b0112 0.42 +0.5 120,753 - aroP b0112 0.42 -1.0 120,847 + aroP b0112 0.49 -1.1 120,854 - aroP b0112 0.49 -0.5 120,905 - aroP b0112 0.53 -0.5 120,905 - aroP b0112 0.53 -0.5 120,956 - aroP b0112 0.57 -0.7 120,956 - aroP b0112 0.57 -1.0 121,032 - aroP b0112 0.62 +0.6 121,032 - aroP b0112 0.62 -0.3 121,032 - aroP b0112 0.62 -0.3 121,054 + aroP b0112 0.64 -0.3 121,087 + aroP b0112 0.66 -0.7 121,095 - aroP b0112 0.67 +0.2 121,128 - aroP b0112 0.69 -0.2 121,128 - aroP b0112 0.69 -0.8 121,146 - aroP b0112 0.70 -0.9 121,146 - aroP b0112 0.70 -0.0 121,266 - aroP b0112 0.79 +1.8 121,266 - aroP b0112 0.79 -0.6 121,335 - aroP b0112 0.84 +0.2 121,374 - aroP b0112 0.87 +0.1 121,374 - aroP b0112 0.87 -2.0 121,431 + -0.0 121,431 + -0.6 121,458 - -1.4 121,469 - +1.6 121,514 + -0.1 121,531 - -1.3 121,716 - +0.3 121,716 - +0.7 121,723 - +1.1 121,723 - +0.2 121,738 - +0.6 122,355 + pdhR b0113 0.34 -2.1 122,358 + pdhR b0113 0.35 -4.0 122,369 + pdhR b0113 0.36 -2.4 122,416 + pdhR b0113 0.42 -0.6 122,442 + pdhR b0113 0.46 -3.6 122,471 + pdhR b0113 0.50 -3.6 122,489 + pdhR b0113 0.52 +0.8 122,489 + pdhR b0113 0.52 -2.1 122,489 + pdhR b0113 0.52 +0.6 122,500 + pdhR b0113 0.53 -3.2 122,537 + pdhR b0113 0.58 -3.0
Or see this region's nucleotide sequence