Experiment: Chloramphenicol 0.0075 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05580 and PS417_05585 are separated by 327 nucleotides PS417_05585 and PS417_05590 are separated by 142 nucleotides PS417_05590 and PS417_05595 overlap by 4 nucleotides
PS417_05580: PS417_05580 - glycerol uptake facilitator GlpF, at 1,256,214 to 1,257,071
_05580
PS417_05585: PS417_05585 - hypothetical protein, at 1,257,399 to 1,257,869
_05585
PS417_05590: PS417_05590 - iron ABC transporter ATP-binding protein, at 1,258,012 to 1,259,121
_05590
PS417_05595: PS417_05595 - ornithine carbamoyltransferase, at 1,259,118 to 1,260,038
_05595
Position (kb)
1258
1259
1260 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1257.167 kb on + strand at 1257.196 kb on - strand at 1257.196 kb on - strand at 1257.201 kb on - strand at 1257.218 kb on + strand at 1257.219 kb on - strand at 1257.277 kb on - strand at 1257.323 kb on - strand at 1257.323 kb on - strand at 1257.337 kb on + strand at 1257.456 kb on + strand, within PS417_05585 at 1257.484 kb on - strand, within PS417_05585 at 1257.484 kb on - strand, within PS417_05585 at 1257.833 kb on + strand at 1257.833 kb on + strand at 1257.929 kb on + strand at 1258.009 kb on + strand at 1258.010 kb on - strand at 1258.010 kb on - strand at 1258.325 kb on + strand, within PS417_05590 at 1258.400 kb on + strand, within PS417_05590 at 1258.400 kb on + strand, within PS417_05590 at 1258.427 kb on + strand, within PS417_05590 at 1258.427 kb on + strand, within PS417_05590 at 1258.458 kb on - strand, within PS417_05590 at 1258.850 kb on + strand, within PS417_05590 at 1258.850 kb on + strand, within PS417_05590 at 1259.108 kb on - strand at 1259.132 kb on - strand at 1259.483 kb on - strand, within PS417_05595 at 1259.535 kb on + strand, within PS417_05595 at 1259.535 kb on + strand, within PS417_05595 at 1259.535 kb on + strand, within PS417_05595 at 1259.535 kb on + strand, within PS417_05595 at 1259.535 kb on + strand, within PS417_05595 at 1259.536 kb on - strand, within PS417_05595 at 1259.536 kb on - strand, within PS417_05595 at 1259.536 kb on - strand, within PS417_05595 at 1259.536 kb on - strand, within PS417_05595 at 1259.539 kb on + strand, within PS417_05595 at 1259.539 kb on + strand, within PS417_05595 at 1259.539 kb on + strand, within PS417_05595 at 1259.539 kb on + strand, within PS417_05595 at 1259.539 kb on + strand, within PS417_05595 at 1259.540 kb on - strand, within PS417_05595 at 1259.540 kb on - strand, within PS417_05595 at 1259.540 kb on - strand, within PS417_05595 at 1259.543 kb on + strand, within PS417_05595 at 1259.544 kb on - strand, within PS417_05595 at 1259.544 kb on - strand, within PS417_05595 at 1259.544 kb on - strand, within PS417_05595 at 1259.544 kb on - strand, within PS417_05595 at 1259.544 kb on - strand, within PS417_05595 at 1259.544 kb on - strand, within PS417_05595 at 1259.544 kb on - strand at 1259.727 kb on - strand, within PS417_05595 at 1259.934 kb on + strand at 1260.042 kb on + strand at 1260.043 kb on - strand at 1260.087 kb on - strand at 1260.087 kb on - strand at 1260.087 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0075 mg/ml remove 1,257,167 + -0.4 1,257,196 - +2.8 1,257,196 - +0.0 1,257,201 - +0.5 1,257,218 + -0.1 1,257,219 - +0.1 1,257,277 - +0.2 1,257,323 - -0.9 1,257,323 - +0.3 1,257,337 + -0.2 1,257,456 + PS417_05585 0.12 -0.1 1,257,484 - PS417_05585 0.18 -0.4 1,257,484 - PS417_05585 0.18 -0.1 1,257,833 + -0.4 1,257,833 + -0.2 1,257,929 + -0.3 1,258,009 + -0.5 1,258,010 - -0.5 1,258,010 - +0.1 1,258,325 + PS417_05590 0.28 -0.6 1,258,400 + PS417_05590 0.35 -0.3 1,258,400 + PS417_05590 0.35 +0.7 1,258,427 + PS417_05590 0.37 +0.0 1,258,427 + PS417_05590 0.37 +0.0 1,258,458 - PS417_05590 0.40 +0.6 1,258,850 + PS417_05590 0.75 -0.3 1,258,850 + PS417_05590 0.75 +0.4 1,259,108 - -0.1 1,259,132 - +0.2 1,259,483 - PS417_05595 0.40 +0.4 1,259,535 + PS417_05595 0.45 -1.3 1,259,535 + PS417_05595 0.45 +0.3 1,259,535 + PS417_05595 0.45 +0.1 1,259,535 + PS417_05595 0.45 -0.5 1,259,535 + PS417_05595 0.45 -2.4 1,259,536 - PS417_05595 0.45 -0.1 1,259,536 - PS417_05595 0.45 -0.3 1,259,536 - PS417_05595 0.45 -0.3 1,259,536 - PS417_05595 0.45 +0.1 1,259,539 + PS417_05595 0.46 +1.2 1,259,539 + PS417_05595 0.46 +0.2 1,259,539 + PS417_05595 0.46 +1.1 1,259,539 + PS417_05595 0.46 +0.2 1,259,539 + PS417_05595 0.46 +0.3 1,259,540 - PS417_05595 0.46 -0.7 1,259,540 - PS417_05595 0.46 +0.6 1,259,540 - PS417_05595 0.46 -0.5 1,259,543 + PS417_05595 0.46 +0.3 1,259,544 - PS417_05595 0.46 +0.4 1,259,544 - PS417_05595 0.46 -0.3 1,259,544 - PS417_05595 0.46 -0.2 1,259,544 - PS417_05595 0.46 +0.2 1,259,544 - PS417_05595 0.46 +0.3 1,259,544 - PS417_05595 0.46 +0.0 1,259,544 - +0.2 1,259,727 - PS417_05595 0.66 +0.5 1,259,934 + -0.8 1,260,042 + +0.0 1,260,043 - +0.9 1,260,087 - +0.2 1,260,087 - +0.0 1,260,087 - +0.4
Or see this region's nucleotide sequence