Strain Fitness in Escherichia coli BW25113 around b3453

Experiment: deuterated glucose in H2O

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntugpE and ugpA overlap by 4 nucleotidesugpA and ugpB are separated by 97 nucleotidesugpB and livF are separated by 398 nucleotides b3451: ugpE - glycerol-3-phosphate transporter subunit (NCBI), at 3,587,205 to 3,588,050 ugpE b3452: ugpA - glycerol-3-phosphate transporter subunit (NCBI), at 3,588,047 to 3,588,934 ugpA b3453: ugpB - glycerol-3-phosphate transporter subunit (NCBI), at 3,589,032 to 3,590,348 ugpB b3454: livF - ATP-binding component of leucine transport (VIMSS), at 3,590,747 to 3,591,460 livF Position (kb) 3589 3590 3591Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3588.047 kb on - strandat 3588.047 kb on - strandat 3588.067 kb on + strandat 3588.238 kb on + strand, within ugpAat 3588.238 kb on + strand, within ugpAat 3588.254 kb on - strand, within ugpAat 3588.269 kb on + strand, within ugpAat 3588.288 kb on + strand, within ugpAat 3588.288 kb on + strand, within ugpAat 3588.291 kb on - strand, within ugpAat 3588.291 kb on - strand, within ugpAat 3588.399 kb on + strand, within ugpAat 3588.404 kb on + strand, within ugpAat 3588.443 kb on + strand, within ugpAat 3588.443 kb on + strand, within ugpAat 3588.540 kb on + strand, within ugpAat 3588.540 kb on + strand, within ugpAat 3588.548 kb on - strand, within ugpAat 3588.588 kb on + strand, within ugpAat 3588.588 kb on + strand, within ugpAat 3588.608 kb on + strand, within ugpAat 3588.638 kb on + strand, within ugpAat 3588.638 kb on + strand, within ugpAat 3588.647 kb on - strand, within ugpAat 3588.647 kb on - strand, within ugpAat 3588.662 kb on - strand, within ugpAat 3588.662 kb on - strand, within ugpAat 3588.667 kb on - strand, within ugpAat 3588.695 kb on - strand, within ugpAat 3588.996 kb on + strandat 3589.030 kb on + strandat 3589.109 kb on - strandat 3589.109 kb on - strandat 3589.121 kb on - strandat 3589.121 kb on - strandat 3589.134 kb on + strandat 3589.134 kb on + strandat 3589.259 kb on + strand, within ugpBat 3589.259 kb on + strand, within ugpBat 3589.282 kb on + strand, within ugpBat 3589.286 kb on - strand, within ugpBat 3589.292 kb on - strand, within ugpBat 3589.292 kb on - strand, within ugpBat 3589.307 kb on + strand, within ugpBat 3589.307 kb on + strand, within ugpBat 3589.415 kb on + strand, within ugpBat 3589.415 kb on + strand, within ugpBat 3589.417 kb on - strand, within ugpBat 3589.417 kb on - strand, within ugpBat 3589.471 kb on + strand, within ugpBat 3589.471 kb on + strand, within ugpBat 3589.484 kb on + strand, within ugpBat 3589.484 kb on + strand, within ugpBat 3589.487 kb on - strand, within ugpBat 3589.540 kb on - strand, within ugpBat 3589.583 kb on - strand, within ugpBat 3589.583 kb on - strand, within ugpBat 3589.629 kb on - strand, within ugpBat 3589.634 kb on + strand, within ugpBat 3589.674 kb on - strand, within ugpBat 3589.674 kb on - strand, within ugpBat 3589.677 kb on + strand, within ugpBat 3589.677 kb on + strand, within ugpBat 3589.685 kb on - strand, within ugpBat 3589.685 kb on - strand, within ugpBat 3589.777 kb on + strand, within ugpBat 3589.777 kb on + strand, within ugpBat 3589.998 kb on - strand, within ugpBat 3589.998 kb on - strand, within ugpBat 3590.230 kb on + strandat 3590.271 kb on + strandat 3590.305 kb on + strandat 3590.319 kb on + strandat 3590.390 kb on + strandat 3590.403 kb on + strandat 3590.411 kb on - strandat 3590.450 kb on + strandat 3590.459 kb on + strandat 3590.655 kb on + strandat 3590.659 kb on - strandat 3590.659 kb on - strandat 3590.703 kb on - strandat 3590.774 kb on - strandat 3590.810 kb on - strandat 3590.898 kb on - strand, within livFat 3590.898 kb on - strand, within livFat 3590.903 kb on - strand, within livFat 3590.966 kb on - strand, within livFat 3590.966 kb on - strand, within livFat 3590.988 kb on + strand, within livFat 3591.059 kb on - strand, within livFat 3591.061 kb on + strand, within livFat 3591.090 kb on - strand, within livFat 3591.125 kb on - strand, within livFat 3591.164 kb on + strand, within livFat 3591.164 kb on + strand, within livFat 3591.172 kb on - strand, within livFat 3591.197 kb on + strand, within livFat 3591.252 kb on + strand, within livFat 3591.252 kb on + strand, within livF

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Per-strain Table

Position Strand Gene LocusTag Fraction deuterated glucose in H2O
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3,588,047 - -0.4
3,588,047 - -1.1
3,588,067 + +0.4
3,588,238 + ugpA b3452 0.22 -0.1
3,588,238 + ugpA b3452 0.22 -0.3
3,588,254 - ugpA b3452 0.23 -0.5
3,588,269 + ugpA b3452 0.25 +0.5
3,588,288 + ugpA b3452 0.27 +0.3
3,588,288 + ugpA b3452 0.27 +0.0
3,588,291 - ugpA b3452 0.27 +0.1
3,588,291 - ugpA b3452 0.27 +1.2
3,588,399 + ugpA b3452 0.40 -0.2
3,588,404 + ugpA b3452 0.40 +0.1
3,588,443 + ugpA b3452 0.45 -0.3
3,588,443 + ugpA b3452 0.45 -1.2
3,588,540 + ugpA b3452 0.56 +1.2
3,588,540 + ugpA b3452 0.56 +0.9
3,588,548 - ugpA b3452 0.56 -1.3
3,588,588 + ugpA b3452 0.61 +0.1
3,588,588 + ugpA b3452 0.61 -0.3
3,588,608 + ugpA b3452 0.63 +2.2
3,588,638 + ugpA b3452 0.67 +0.5
3,588,638 + ugpA b3452 0.67 +0.3
3,588,647 - ugpA b3452 0.68 -0.3
3,588,647 - ugpA b3452 0.68 -1.4
3,588,662 - ugpA b3452 0.69 -1.5
3,588,662 - ugpA b3452 0.69 -0.5
3,588,667 - ugpA b3452 0.70 -0.7
3,588,695 - ugpA b3452 0.73 +0.3
3,588,996 + +0.0
3,589,030 + +0.4
3,589,109 - +0.9
3,589,109 - +0.2
3,589,121 - +1.2
3,589,121 - +0.4
3,589,134 + +0.4
3,589,134 + +1.4
3,589,259 + ugpB b3453 0.17 +1.4
3,589,259 + ugpB b3453 0.17 -0.6
3,589,282 + ugpB b3453 0.19 -0.2
3,589,286 - ugpB b3453 0.19 -0.2
3,589,292 - ugpB b3453 0.20 +0.8
3,589,292 - ugpB b3453 0.20 -1.2
3,589,307 + ugpB b3453 0.21 -0.0
3,589,307 + ugpB b3453 0.21 -0.4
3,589,415 + ugpB b3453 0.29 -0.4
3,589,415 + ugpB b3453 0.29 +0.4
3,589,417 - ugpB b3453 0.29 +0.2
3,589,417 - ugpB b3453 0.29 +0.3
3,589,471 + ugpB b3453 0.33 -0.6
3,589,471 + ugpB b3453 0.33 -0.3
3,589,484 + ugpB b3453 0.34 +0.4
3,589,484 + ugpB b3453 0.34 +0.1
3,589,487 - ugpB b3453 0.35 -1.0
3,589,540 - ugpB b3453 0.39 -0.1
3,589,583 - ugpB b3453 0.42 +1.1
3,589,583 - ugpB b3453 0.42 +0.2
3,589,629 - ugpB b3453 0.45 +0.0
3,589,634 + ugpB b3453 0.46 +0.3
3,589,674 - ugpB b3453 0.49 -1.5
3,589,674 - ugpB b3453 0.49 -0.1
3,589,677 + ugpB b3453 0.49 -0.4
3,589,677 + ugpB b3453 0.49 +0.3
3,589,685 - ugpB b3453 0.50 -0.1
3,589,685 - ugpB b3453 0.50 +0.0
3,589,777 + ugpB b3453 0.57 -2.0
3,589,777 + ugpB b3453 0.57 +0.3
3,589,998 - ugpB b3453 0.73 -0.6
3,589,998 - ugpB b3453 0.73 -0.2
3,590,230 + -0.0
3,590,271 + +0.8
3,590,305 + -0.6
3,590,319 + +0.1
3,590,390 + -0.4
3,590,403 + +2.2
3,590,411 - +1.6
3,590,450 + +0.4
3,590,459 + +3.2
3,590,655 + +0.9
3,590,659 - +0.3
3,590,659 - -0.2
3,590,703 - +0.5
3,590,774 - +2.0
3,590,810 - +1.7
3,590,898 - livF b3454 0.21 +0.6
3,590,898 - livF b3454 0.21 +0.6
3,590,903 - livF b3454 0.22 +1.7
3,590,966 - livF b3454 0.31 +0.5
3,590,966 - livF b3454 0.31 +1.0
3,590,988 + livF b3454 0.34 +2.5
3,591,059 - livF b3454 0.44 +1.8
3,591,061 + livF b3454 0.44 +1.8
3,591,090 - livF b3454 0.48 +1.9
3,591,125 - livF b3454 0.53 +2.4
3,591,164 + livF b3454 0.58 +2.2
3,591,164 + livF b3454 0.58 +1.4
3,591,172 - livF b3454 0.60 +1.8
3,591,197 + livF b3454 0.63 +2.9
3,591,252 + livF b3454 0.71 +2.7
3,591,252 + livF b3454 0.71 +2.3

Or see this region's nucleotide sequence