Experiment: deuterated glucose in H2O
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybgI and ybgJ are separated by 22 nucleotides ybgJ and ybgK overlap by 7 nucleotides ybgK and ybgL overlap by 11 nucleotides ybgL and nei are separated by 35 nucleotides
b0710: ybgI - conserved metal-binding protein (NCBI), at 742,050 to 742,793
ybgI
b0711: ybgJ - predicted enzyme subunit (NCBI), at 742,816 to 743,472
ybgJ
b0712: ybgK - predicted enzyme subunit (NCBI), at 743,466 to 744,398
ybgK
b0713: ybgL - hypothetical protein (NCBI), at 744,388 to 745,122
ybgL
b0714: nei - endonuclease VIII/ 5-formyluracil/5-hydroxymethyluracil DNA glycosylase (NCBI), at 745,158 to 745,949
nei
Position (kb) 743
744
745 Strain fitness (log2 ratio) -1
0
1
2 at 742.541 kb on + strand, within ybgI at 742.541 kb on + strand, within ybgI at 742.615 kb on + strand, within ybgI at 742.699 kb on - strand, within ybgI at 742.753 kb on + strand at 742.761 kb on - strand at 742.761 kb on - strand at 742.973 kb on - strand, within ybgJ at 743.091 kb on + strand, within ybgJ at 743.091 kb on + strand, within ybgJ at 743.091 kb on + strand, within ybgJ at 743.185 kb on - strand, within ybgJ at 743.185 kb on - strand, within ybgJ at 743.185 kb on - strand, within ybgJ at 743.248 kb on - strand, within ybgJ at 743.250 kb on + strand, within ybgJ at 743.250 kb on + strand, within ybgJ at 743.302 kb on + strand, within ybgJ at 743.369 kb on + strand, within ybgJ at 743.374 kb on + strand, within ybgJ at 743.461 kb on + strand at 743.489 kb on + strand at 743.492 kb on + strand at 743.492 kb on + strand at 743.492 kb on + strand at 743.500 kb on - strand at 743.500 kb on - strand at 743.504 kb on + strand at 743.515 kb on - strand at 743.538 kb on + strand at 743.538 kb on + strand at 743.539 kb on + strand at 743.539 kb on + strand at 743.595 kb on - strand, within ybgK at 743.595 kb on - strand, within ybgK at 743.642 kb on + strand, within ybgK at 743.799 kb on + strand, within ybgK at 743.865 kb on - strand, within ybgK at 743.880 kb on + strand, within ybgK at 743.880 kb on + strand, within ybgK at 743.888 kb on - strand, within ybgK at 743.920 kb on - strand, within ybgK at 743.946 kb on - strand, within ybgK at 743.956 kb on + strand, within ybgK at 743.956 kb on + strand, within ybgK at 744.039 kb on + strand, within ybgK at 744.039 kb on + strand, within ybgK at 744.080 kb on + strand, within ybgK at 744.080 kb on + strand, within ybgK at 744.152 kb on + strand, within ybgK at 744.271 kb on - strand, within ybgK at 744.423 kb on - strand at 744.662 kb on - strand, within ybgL at 744.662 kb on - strand, within ybgL at 744.711 kb on + strand, within ybgL at 744.766 kb on + strand, within ybgL at 744.832 kb on - strand, within ybgL at 744.978 kb on - strand, within ybgL at 744.978 kb on - strand, within ybgL at 745.140 kb on - strand at 745.148 kb on + strand at 745.194 kb on + strand at 745.194 kb on + strand at 745.286 kb on + strand, within nei at 745.297 kb on + strand, within nei at 745.297 kb on + strand, within nei at 745.358 kb on - strand, within nei at 745.358 kb on - strand, within nei
Per-strain Table
Position Strand Gene LocusTag Fraction deuterated glucose in H2O remove 742,541 + ybgI b0710 0.66 -0.7 742,541 + ybgI b0710 0.66 -1.2 742,615 + ybgI b0710 0.76 +1.1 742,699 - ybgI b0710 0.87 +0.5 742,753 + -1.1 742,761 - +1.0 742,761 - +0.4 742,973 - ybgJ b0711 0.24 +1.1 743,091 + ybgJ b0711 0.42 +1.1 743,091 + ybgJ b0711 0.42 -0.4 743,091 + ybgJ b0711 0.42 +2.3 743,185 - ybgJ b0711 0.56 +1.2 743,185 - ybgJ b0711 0.56 -0.1 743,185 - ybgJ b0711 0.56 +0.0 743,248 - ybgJ b0711 0.66 +0.8 743,250 + ybgJ b0711 0.66 -0.5 743,250 + ybgJ b0711 0.66 +0.4 743,302 + ybgJ b0711 0.74 +1.4 743,369 + ybgJ b0711 0.84 +1.0 743,374 + ybgJ b0711 0.85 +0.2 743,461 + -0.2 743,489 + +0.7 743,492 + -0.1 743,492 + +0.6 743,492 + +0.5 743,500 - -0.5 743,500 - -0.5 743,504 + +0.7 743,515 - +0.7 743,538 + +1.4 743,538 + +0.7 743,539 + +1.7 743,539 + -0.3 743,595 - ybgK b0712 0.14 +0.7 743,595 - ybgK b0712 0.14 -1.5 743,642 + ybgK b0712 0.19 +0.2 743,799 + ybgK b0712 0.36 +0.3 743,865 - ybgK b0712 0.43 -0.8 743,880 + ybgK b0712 0.44 +0.6 743,880 + ybgK b0712 0.44 +0.2 743,888 - ybgK b0712 0.45 +1.0 743,920 - ybgK b0712 0.49 +1.0 743,946 - ybgK b0712 0.51 +0.7 743,956 + ybgK b0712 0.53 +0.7 743,956 + ybgK b0712 0.53 -0.1 744,039 + ybgK b0712 0.61 -0.1 744,039 + ybgK b0712 0.61 +0.2 744,080 + ybgK b0712 0.66 +1.2 744,080 + ybgK b0712 0.66 -0.4 744,152 + ybgK b0712 0.74 -0.3 744,271 - ybgK b0712 0.86 +1.7 744,423 - +0.7 744,662 - ybgL b0713 0.37 +0.4 744,662 - ybgL b0713 0.37 +0.9 744,711 + ybgL b0713 0.44 -0.1 744,766 + ybgL b0713 0.51 +0.9 744,832 - ybgL b0713 0.60 -0.1 744,978 - ybgL b0713 0.80 +0.2 744,978 - ybgL b0713 0.80 +0.5 745,140 - +0.3 745,148 + +0.9 745,194 + -0.2 745,194 + +0.1 745,286 + nei b0714 0.16 +0.7 745,297 + nei b0714 0.18 +0.5 745,297 + nei b0714 0.18 +0.5 745,358 - nei b0714 0.25 +0.6 745,358 - nei b0714 0.25 +1.7
Or see this region's nucleotide sequence