Strain Fitness in Escherichia coli BW25113 around b0107

Experiment: deuterated glucose in H2O

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthofC and hofB overlap by 11 nucleotideshofB and ppdD are separated by 9 nucleotidesppdD and nadC are separated by 202 nucleotides b0106: hofC - assembly protein in type IV pilin biogenesis, transmembrane protein (NCBI), at 114,522 to 115,724 hofC b0107: hofB - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 115,714 to 117,099 hofB b0108: ppdD - predicted major pilin subunit (NCBI), at 117,109 to 117,549 ppdD b0109: nadC - nicotinate-nucleotide pyrophosphorylase (NCBI), at 117,752 to 118,645 nadC Position (kb) 115 116 117 118Strain fitness (log2 ratio) -2 -1 0 1 2at 114.838 kb on + strand, within hofCat 114.838 kb on + strand, within hofCat 114.844 kb on + strand, within hofCat 114.928 kb on - strand, within hofCat 114.930 kb on - strandat 114.930 kb on - strand, within hofCat 114.998 kb on + strand, within hofCat 114.998 kb on + strand, within hofCat 115.010 kb on - strand, within hofCat 115.021 kb on + strand, within hofCat 115.021 kb on + strand, within hofCat 115.077 kb on - strand, within hofCat 115.152 kb on - strand, within hofCat 115.152 kb on - strand, within hofCat 115.201 kb on - strand, within hofCat 115.203 kb on - strand, within hofCat 115.203 kb on - strand, within hofCat 115.273 kb on + strand, within hofCat 115.278 kb on + strand, within hofCat 115.278 kb on + strand, within hofCat 115.330 kb on + strand, within hofCat 115.367 kb on + strand, within hofCat 115.367 kb on + strand, within hofCat 115.379 kb on + strand, within hofCat 115.379 kb on + strand, within hofCat 115.409 kb on + strand, within hofCat 115.474 kb on - strand, within hofCat 115.507 kb on + strand, within hofCat 115.507 kb on + strand, within hofCat 115.515 kb on - strand, within hofCat 115.624 kb on + strandat 115.651 kb on + strandat 115.699 kb on - strandat 115.737 kb on + strandat 115.856 kb on - strand, within hofBat 115.856 kb on - strand, within hofBat 115.910 kb on + strand, within hofBat 115.911 kb on - strand, within hofBat 115.911 kb on - strand, within hofBat 115.941 kb on + strand, within hofBat 115.980 kb on + strand, within hofBat 116.058 kb on + strand, within hofBat 116.149 kb on - strand, within hofBat 116.335 kb on + strand, within hofBat 116.399 kb on + strand, within hofBat 116.430 kb on - strand, within hofBat 116.430 kb on - strand, within hofBat 116.471 kb on - strand, within hofBat 116.760 kb on - strand, within hofBat 116.777 kb on + strand, within hofBat 116.777 kb on + strand, within hofBat 116.872 kb on + strand, within hofBat 116.882 kb on + strand, within hofBat 116.957 kb on - strand, within hofBat 116.964 kb on - strandat 117.046 kb on + strandat 117.056 kb on + strandat 117.069 kb on + strandat 117.075 kb on + strandat 117.075 kb on + strandat 117.075 kb on + strandat 117.081 kb on - strandat 117.081 kb on - strandat 117.084 kb on + strandat 117.087 kb on + strandat 117.227 kb on - strand, within ppdDat 117.281 kb on + strand, within ppdDat 117.281 kb on + strand, within ppdDat 117.293 kb on - strand, within ppdDat 117.302 kb on + strand, within ppdDat 117.302 kb on + strand, within ppdDat 117.385 kb on + strand, within ppdDat 117.387 kb on + strand, within ppdDat 117.508 kb on - strandat 117.508 kb on - strandat 117.511 kb on - strandat 117.511 kb on - strandat 117.525 kb on - strandat 117.525 kb on - strandat 117.603 kb on + strandat 117.603 kb on + strandat 117.839 kb on + strandat 117.856 kb on - strand, within nadCat 117.856 kb on - strand, within nadCat 117.879 kb on - strand, within nadCat 117.879 kb on - strand, within nadCat 117.881 kb on - strand, within nadCat 117.922 kb on - strand, within nadCat 117.922 kb on - strand, within nadC

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Per-strain Table

Position Strand Gene LocusTag Fraction deuterated glucose in H2O
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114,838 + hofC b0106 0.26 -0.4
114,838 + hofC b0106 0.26 -0.1
114,844 + hofC b0106 0.27 +0.2
114,928 - hofC b0106 0.34 +0.1
114,930 - +2.0
114,930 - hofC b0106 0.34 -0.5
114,998 + hofC b0106 0.40 +0.1
114,998 + hofC b0106 0.40 +0.4
115,010 - hofC b0106 0.41 -0.9
115,021 + hofC b0106 0.41 -0.8
115,021 + hofC b0106 0.41 -0.8
115,077 - hofC b0106 0.46 -0.2
115,152 - hofC b0106 0.52 +0.1
115,152 - hofC b0106 0.52 +0.7
115,201 - hofC b0106 0.56 -0.3
115,203 - hofC b0106 0.57 -0.5
115,203 - hofC b0106 0.57 -2.7
115,273 + hofC b0106 0.62 -0.5
115,278 + hofC b0106 0.63 +0.3
115,278 + hofC b0106 0.63 +0.1
115,330 + hofC b0106 0.67 -0.9
115,367 + hofC b0106 0.70 +0.1
115,367 + hofC b0106 0.70 +0.5
115,379 + hofC b0106 0.71 -0.1
115,379 + hofC b0106 0.71 +0.4
115,409 + hofC b0106 0.74 -0.0
115,474 - hofC b0106 0.79 -1.5
115,507 + hofC b0106 0.82 +1.1
115,507 + hofC b0106 0.82 +0.2
115,515 - hofC b0106 0.83 +0.8
115,624 + +0.8
115,651 + -0.5
115,699 - +1.4
115,737 + +0.0
115,856 - hofB b0107 0.10 -0.7
115,856 - hofB b0107 0.10 -1.8
115,910 + hofB b0107 0.14 -0.4
115,911 - hofB b0107 0.14 -0.9
115,911 - hofB b0107 0.14 -1.7
115,941 + hofB b0107 0.16 -0.6
115,980 + hofB b0107 0.19 -1.1
116,058 + hofB b0107 0.25 +1.1
116,149 - hofB b0107 0.31 +0.2
116,335 + hofB b0107 0.45 -0.4
116,399 + hofB b0107 0.49 -1.7
116,430 - hofB b0107 0.52 -0.2
116,430 - hofB b0107 0.52 +0.2
116,471 - hofB b0107 0.55 -1.7
116,760 - hofB b0107 0.75 -0.2
116,777 + hofB b0107 0.77 +2.3
116,777 + hofB b0107 0.77 -1.3
116,872 + hofB b0107 0.84 +0.0
116,882 + hofB b0107 0.84 -1.9
116,957 - hofB b0107 0.90 -0.4
116,964 - +1.2
117,046 + -0.4
117,056 + +0.1
117,069 + -0.2
117,075 + +0.7
117,075 + +0.6
117,075 + +0.5
117,081 - -0.1
117,081 - -0.5
117,084 + +0.8
117,087 + -0.4
117,227 - ppdD b0108 0.27 +0.7
117,281 + ppdD b0108 0.39 +0.4
117,281 + ppdD b0108 0.39 +0.5
117,293 - ppdD b0108 0.42 +0.4
117,302 + ppdD b0108 0.44 -0.5
117,302 + ppdD b0108 0.44 -0.6
117,385 + ppdD b0108 0.63 +1.2
117,387 + ppdD b0108 0.63 -1.3
117,508 - -0.5
117,508 - -0.2
117,511 - +0.1
117,511 - -0.1
117,525 - +1.0
117,525 - +0.4
117,603 + +1.0
117,603 + +0.9
117,839 + -1.2
117,856 - nadC b0109 0.12 -1.0
117,856 - nadC b0109 0.12 -0.5
117,879 - nadC b0109 0.14 +2.0
117,879 - nadC b0109 0.14 -1.7
117,881 - nadC b0109 0.14 -1.7
117,922 - nadC b0109 0.19 -1.9
117,922 - nadC b0109 0.19 -1.3

Or see this region's nucleotide sequence