Experiment: deuterated glucose in H2O
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phnM and phnL overlap by 4 nucleotides phnL and phnK are separated by 110 nucleotides phnK and phnJ overlap by 4 nucleotides
b4095: phnM - carbon-phosphorus lyase complex subunit (NCBI), at 4,314,105 to 4,315,241
phnM
b4096: phnL - carbon-phosphorus lyase complex subunit (NCBI), at 4,315,238 to 4,315,918
phnL
b4097: phnK - carbon-phosphorus lyase complex subunit (NCBI), at 4,316,029 to 4,316,787
phnK
b4098: phnJ - carbon-phosphorus lyase complex subunit (NCBI), at 4,316,784 to 4,317,629
phnJ
Position (kb)
4315
4316 Strain fitness (log2 ratio)
-1
0
1
2
3 at 4314.357 kb on - strand, within phnM at 4314.363 kb on + strand, within phnM at 4314.396 kb on + strand, within phnM at 4314.445 kb on + strand, within phnM at 4314.445 kb on + strand, within phnM at 4314.742 kb on + strand, within phnM at 4314.819 kb on + strand, within phnM at 4314.819 kb on + strand, within phnM at 4314.822 kb on - strand, within phnM at 4314.822 kb on - strand, within phnM at 4314.857 kb on - strand, within phnM at 4314.857 kb on - strand, within phnM at 4315.006 kb on - strand, within phnM at 4315.181 kb on - strand at 4315.192 kb on - strand at 4315.192 kb on - strand at 4315.200 kb on - strand at 4315.288 kb on - strand at 4315.408 kb on + strand, within phnL at 4315.507 kb on + strand, within phnL at 4315.516 kb on - strand, within phnL at 4315.516 kb on - strand, within phnL at 4315.585 kb on - strand, within phnL at 4315.585 kb on - strand, within phnL at 4315.720 kb on - strand, within phnL at 4315.740 kb on + strand, within phnL at 4315.740 kb on + strand, within phnL at 4315.751 kb on + strand, within phnL at 4316.095 kb on + strand at 4316.185 kb on + strand, within phnK at 4316.185 kb on + strand, within phnK at 4316.208 kb on - strand, within phnK at 4316.211 kb on + strand, within phnK at 4316.242 kb on + strand, within phnK at 4316.242 kb on + strand, within phnK at 4316.434 kb on + strand, within phnK at 4316.434 kb on + strand, within phnK at 4316.447 kb on - strand, within phnK at 4316.461 kb on - strand, within phnK at 4316.564 kb on + strand, within phnK at 4316.564 kb on + strand, within phnK at 4316.654 kb on - strand, within phnK at 4316.891 kb on - strand, within phnJ
Per-strain Table
Position Strand Gene LocusTag Fraction deuterated glucose in H2O remove 4,314,357 - phnM b4095 0.22 +0.8 4,314,363 + phnM b4095 0.23 +1.7 4,314,396 + phnM b4095 0.26 +1.5 4,314,445 + phnM b4095 0.30 +1.1 4,314,445 + phnM b4095 0.30 +1.5 4,314,742 + phnM b4095 0.56 +0.7 4,314,819 + phnM b4095 0.63 +1.7 4,314,819 + phnM b4095 0.63 +1.1 4,314,822 - phnM b4095 0.63 +0.7 4,314,822 - phnM b4095 0.63 +1.1 4,314,857 - phnM b4095 0.66 +1.0 4,314,857 - phnM b4095 0.66 +0.7 4,315,006 - phnM b4095 0.79 +1.6 4,315,181 - +0.4 4,315,192 - +0.7 4,315,192 - +0.7 4,315,200 - +1.3 4,315,288 - +1.3 4,315,408 + phnL b4096 0.25 +0.5 4,315,507 + phnL b4096 0.40 +1.6 4,315,516 - phnL b4096 0.41 +1.8 4,315,516 - phnL b4096 0.41 +1.0 4,315,585 - phnL b4096 0.51 +2.7 4,315,585 - phnL b4096 0.51 +1.7 4,315,720 - phnL b4096 0.71 +2.7 4,315,740 + phnL b4096 0.74 +0.4 4,315,740 + phnL b4096 0.74 +2.5 4,315,751 + phnL b4096 0.75 +0.9 4,316,095 + +2.7 4,316,185 + phnK b4097 0.21 +0.3 4,316,185 + phnK b4097 0.21 +1.7 4,316,208 - phnK b4097 0.24 +1.5 4,316,211 + phnK b4097 0.24 +0.9 4,316,242 + phnK b4097 0.28 +1.7 4,316,242 + phnK b4097 0.28 +2.0 4,316,434 + phnK b4097 0.53 +0.1 4,316,434 + phnK b4097 0.53 +0.8 4,316,447 - phnK b4097 0.55 +2.8 4,316,461 - phnK b4097 0.57 +1.0 4,316,564 + phnK b4097 0.70 +0.8 4,316,564 + phnK b4097 0.70 +1.6 4,316,654 - phnK b4097 0.82 +0.8 4,316,891 - phnJ b4098 0.13 +0.9
Or see this region's nucleotide sequence