Experiment: deuterated glucose in H2O
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pflD and pflC overlap by 35 nucleotides pflC and frwD are separated by 1 nucleotides frwD and yijO overlap by 14 nucleotides
b3951: pflD - predicted formate acetyltransferase 2 (pyruvate formate lyase II) (NCBI), at 4,142,018 to 4,144,315
pflD
b3952: pflC - probable pyruvate formate lyase activating enzyme 2 (VIMSS), at 4,144,281 to 4,145,159
pflC
b3953: frwD - predicted enzyme IIB component of PTS (NCBI), at 4,145,161 to 4,145,502
frwD
b3954: yijO - predicted DNA-binding transcriptional regulator (NCBI), at 4,145,489 to 4,146,340
yijO
Position (kb)
4145
4146 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4144.162 kb on - strand at 4144.235 kb on + strand at 4144.235 kb on + strand at 4144.243 kb on - strand at 4144.243 kb on - strand at 4144.287 kb on - strand at 4144.304 kb on - strand at 4144.317 kb on - strand at 4144.317 kb on - strand at 4144.409 kb on + strand, within pflC at 4144.445 kb on - strand, within pflC at 4144.454 kb on + strand, within pflC at 4144.454 kb on + strand, within pflC at 4144.479 kb on + strand, within pflC at 4144.479 kb on + strand, within pflC at 4144.505 kb on - strand, within pflC at 4144.592 kb on - strand, within pflC at 4144.592 kb on - strand, within pflC at 4144.599 kb on + strand, within pflC at 4144.599 kb on + strand, within pflC at 4144.709 kb on + strand, within pflC at 4144.770 kb on - strand, within pflC at 4144.841 kb on + strand, within pflC at 4144.952 kb on + strand, within pflC at 4144.983 kb on - strand, within pflC at 4144.983 kb on - strand, within pflC at 4145.095 kb on - strand at 4145.097 kb on + strand at 4145.097 kb on + strand at 4145.109 kb on - strand at 4145.142 kb on - strand at 4145.142 kb on - strand at 4145.142 kb on - strand at 4145.190 kb on + strand at 4145.190 kb on + strand at 4145.261 kb on - strand, within frwD at 4145.263 kb on - strand, within frwD at 4145.264 kb on - strand, within frwD at 4145.341 kb on - strand, within frwD at 4145.354 kb on + strand, within frwD at 4145.354 kb on + strand, within frwD at 4145.364 kb on + strand, within frwD at 4145.364 kb on + strand, within frwD at 4145.372 kb on - strand, within frwD at 4145.398 kb on + strand, within frwD at 4145.398 kb on + strand, within frwD at 4145.411 kb on - strand, within frwD at 4145.419 kb on + strand, within frwD at 4145.452 kb on + strand, within frwD at 4145.499 kb on - strand at 4145.500 kb on - strand at 4145.525 kb on + strand at 4145.612 kb on + strand, within yijO at 4145.626 kb on - strand, within yijO at 4145.638 kb on + strand, within yijO at 4145.658 kb on + strand, within yijO at 4145.658 kb on + strand, within yijO at 4145.669 kb on + strand, within yijO at 4145.669 kb on + strand, within yijO at 4145.677 kb on - strand, within yijO at 4145.697 kb on - strand, within yijO at 4145.697 kb on - strand, within yijO at 4145.800 kb on - strand, within yijO at 4145.843 kb on + strand, within yijO at 4145.853 kb on + strand, within yijO at 4145.856 kb on - strand, within yijO at 4145.900 kb on + strand, within yijO at 4145.900 kb on + strand, within yijO at 4146.038 kb on + strand, within yijO at 4146.069 kb on + strand, within yijO at 4146.091 kb on - strand, within yijO at 4146.093 kb on + strand, within yijO at 4146.137 kb on + strand, within yijO at 4146.140 kb on + strand, within yijO at 4146.148 kb on + strand, within yijO at 4146.162 kb on + strand, within yijO at 4146.162 kb on + strand, within yijO at 4146.168 kb on + strand, within yijO at 4146.174 kb on + strand, within yijO at 4146.179 kb on + strand, within yijO at 4146.207 kb on + strand, within yijO at 4146.238 kb on - strand, within yijO at 4146.242 kb on + strand, within yijO at 4146.290 kb on + strand at 4146.338 kb on - strand at 4146.347 kb on - strand at 4146.359 kb on - strand at 4146.419 kb on - strand at 4146.457 kb on + strand at 4146.458 kb on - strand at 4146.458 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction deuterated glucose in H2O remove 4,144,162 - +0.2 4,144,235 + -1.2 4,144,235 + +0.4 4,144,243 - +0.9 4,144,243 - +0.0 4,144,287 - +1.2 4,144,304 - +0.4 4,144,317 - +1.1 4,144,317 - +0.4 4,144,409 + pflC b3952 0.15 -0.9 4,144,445 - pflC b3952 0.19 -1.9 4,144,454 + pflC b3952 0.20 -0.7 4,144,454 + pflC b3952 0.20 +1.6 4,144,479 + pflC b3952 0.23 +0.3 4,144,479 + pflC b3952 0.23 -0.5 4,144,505 - pflC b3952 0.25 +0.1 4,144,592 - pflC b3952 0.35 -0.4 4,144,592 - pflC b3952 0.35 -0.4 4,144,599 + pflC b3952 0.36 +0.6 4,144,599 + pflC b3952 0.36 +0.3 4,144,709 + pflC b3952 0.49 -0.4 4,144,770 - pflC b3952 0.56 -0.3 4,144,841 + pflC b3952 0.64 +0.7 4,144,952 + pflC b3952 0.76 +1.6 4,144,983 - pflC b3952 0.80 -0.1 4,144,983 - pflC b3952 0.80 -1.5 4,145,095 - -0.5 4,145,097 + -2.6 4,145,097 + +0.1 4,145,109 - +0.1 4,145,142 - +0.8 4,145,142 - +0.8 4,145,142 - -0.0 4,145,190 + -1.0 4,145,190 + +0.9 4,145,261 - frwD b3953 0.29 -0.8 4,145,263 - frwD b3953 0.30 +0.7 4,145,264 - frwD b3953 0.30 -1.7 4,145,341 - frwD b3953 0.53 -0.4 4,145,354 + frwD b3953 0.56 -0.4 4,145,354 + frwD b3953 0.56 -1.3 4,145,364 + frwD b3953 0.59 +0.5 4,145,364 + frwD b3953 0.59 -0.8 4,145,372 - frwD b3953 0.62 +0.5 4,145,398 + frwD b3953 0.69 -1.5 4,145,398 + frwD b3953 0.69 -0.8 4,145,411 - frwD b3953 0.73 +0.1 4,145,419 + frwD b3953 0.75 +1.3 4,145,452 + frwD b3953 0.85 -1.9 4,145,499 - -0.5 4,145,500 - +2.1 4,145,525 + +0.6 4,145,612 + yijO b3954 0.14 +1.1 4,145,626 - yijO b3954 0.16 -1.3 4,145,638 + yijO b3954 0.17 -1.7 4,145,658 + yijO b3954 0.20 -0.8 4,145,658 + yijO b3954 0.20 -2.2 4,145,669 + yijO b3954 0.21 +0.9 4,145,669 + yijO b3954 0.21 +0.2 4,145,677 - yijO b3954 0.22 +1.5 4,145,697 - yijO b3954 0.24 -0.0 4,145,697 - yijO b3954 0.24 +0.1 4,145,800 - yijO b3954 0.37 +1.9 4,145,843 + yijO b3954 0.42 -0.3 4,145,853 + yijO b3954 0.43 -1.8 4,145,856 - yijO b3954 0.43 -0.4 4,145,900 + yijO b3954 0.48 +0.8 4,145,900 + yijO b3954 0.48 +0.1 4,146,038 + yijO b3954 0.64 +1.1 4,146,069 + yijO b3954 0.68 -0.2 4,146,091 - yijO b3954 0.71 +0.4 4,146,093 + yijO b3954 0.71 +1.1 4,146,137 + yijO b3954 0.76 +0.3 4,146,140 + yijO b3954 0.76 -1.1 4,146,148 + yijO b3954 0.77 +1.9 4,146,162 + yijO b3954 0.79 +0.5 4,146,162 + yijO b3954 0.79 -1.8 4,146,168 + yijO b3954 0.80 +0.3 4,146,174 + yijO b3954 0.80 +0.3 4,146,179 + yijO b3954 0.81 +0.6 4,146,207 + yijO b3954 0.84 +0.6 4,146,238 - yijO b3954 0.88 -0.7 4,146,242 + yijO b3954 0.88 -0.9 4,146,290 + -0.5 4,146,338 - -0.6 4,146,347 - -0.7 4,146,359 - +0.1 4,146,419 - +1.0 4,146,457 + +0.8 4,146,458 - -0.2 4,146,458 - -1.0
Or see this region's nucleotide sequence