Experiment: deuterated glucose in H2O
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhjY and tag are separated by 157 nucleotides tag and yiaC overlap by 4 nucleotides yiaC and bisC overlap by 32 nucleotides
b3548: yhjY - putative lipase (VIMSS), at 3,710,259 to 3,710,957
yhjY
b3549: tag - 3-methyl-adenine DNA glycosylase I, constitutive (NCBI), at 3,711,115 to 3,711,678
tag
b3550: yiaC - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 3,711,675 to 3,712,115
yiaC
b3551: bisC - biotin sulfoxide reductase (RefSeq), at 3,712,084 to 3,714,417
bisC
Position (kb)
3711
3712
3713 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3710.694 kb on - strand, within yhjY at 3710.703 kb on - strand, within yhjY at 3710.729 kb on + strand, within yhjY at 3710.732 kb on - strand, within yhjY at 3710.779 kb on - strand, within yhjY at 3710.797 kb on - strand, within yhjY at 3710.798 kb on - strand, within yhjY at 3710.799 kb on - strand, within yhjY at 3710.800 kb on + strand, within yhjY at 3710.800 kb on + strand, within yhjY at 3710.800 kb on + strand, within yhjY at 3710.805 kb on + strand, within yhjY at 3710.805 kb on + strand, within yhjY at 3710.805 kb on + strand, within yhjY at 3710.805 kb on + strand, within yhjY at 3710.820 kb on - strand, within yhjY at 3710.859 kb on - strand, within yhjY at 3710.868 kb on + strand, within yhjY at 3710.917 kb on + strand at 3710.919 kb on + strand at 3710.966 kb on + strand at 3710.966 kb on + strand at 3710.966 kb on + strand at 3710.966 kb on - strand at 3710.966 kb on - strand at 3710.974 kb on - strand at 3711.005 kb on + strand at 3711.005 kb on + strand at 3711.010 kb on - strand at 3711.011 kb on - strand at 3711.019 kb on - strand at 3711.042 kb on - strand at 3711.091 kb on + strand at 3711.123 kb on + strand at 3711.137 kb on + strand at 3711.137 kb on + strand at 3711.155 kb on - strand at 3711.162 kb on + strand at 3711.162 kb on + strand at 3711.183 kb on + strand, within tag at 3711.183 kb on + strand, within tag at 3711.229 kb on + strand, within tag at 3711.298 kb on - strand, within tag at 3711.298 kb on - strand at 3711.352 kb on + strand, within tag at 3711.357 kb on - strand, within tag at 3711.357 kb on - strand, within tag at 3711.360 kb on - strand, within tag at 3711.360 kb on - strand, within tag at 3711.421 kb on - strand, within tag at 3711.433 kb on - strand, within tag at 3711.490 kb on - strand, within tag at 3711.498 kb on + strand, within tag at 3711.500 kb on - strand, within tag at 3711.534 kb on - strand, within tag at 3711.534 kb on - strand, within tag at 3711.613 kb on - strand, within tag at 3711.613 kb on - strand, within tag at 3711.617 kb on + strand, within tag at 3711.617 kb on + strand, within tag at 3711.617 kb on + strand, within tag at 3711.625 kb on - strand at 3711.635 kb on - strand at 3711.645 kb on - strand at 3711.645 kb on - strand at 3711.648 kb on + strand at 3711.702 kb on - strand at 3711.739 kb on - strand, within yiaC at 3711.751 kb on + strand, within yiaC at 3711.751 kb on + strand, within yiaC at 3711.786 kb on - strand, within yiaC at 3711.873 kb on - strand, within yiaC at 3711.873 kb on - strand, within yiaC at 3711.873 kb on - strand, within yiaC at 3711.874 kb on - strand, within yiaC at 3711.923 kb on - strand, within yiaC at 3711.946 kb on + strand, within yiaC at 3711.946 kb on + strand, within yiaC at 3711.949 kb on + strand, within yiaC at 3711.954 kb on - strand, within yiaC at 3711.954 kb on - strand, within yiaC at 3711.970 kb on + strand, within yiaC at 3711.973 kb on - strand, within yiaC at 3712.058 kb on + strand, within yiaC at 3712.073 kb on + strand at 3712.116 kb on - strand at 3712.118 kb on - strand at 3712.118 kb on - strand at 3712.135 kb on + strand at 3712.135 kb on + strand at 3712.174 kb on + strand at 3712.191 kb on + strand at 3712.191 kb on + strand at 3712.236 kb on - strand at 3712.236 kb on - strand at 3712.305 kb on + strand at 3712.320 kb on + strand, within bisC at 3712.320 kb on + strand, within bisC at 3712.349 kb on + strand, within bisC at 3712.357 kb on - strand, within bisC at 3712.364 kb on + strand, within bisC at 3712.364 kb on + strand, within bisC at 3712.374 kb on - strand, within bisC at 3712.374 kb on - strand, within bisC at 3712.382 kb on + strand, within bisC at 3712.405 kb on - strand at 3712.408 kb on + strand, within bisC at 3712.411 kb on - strand, within bisC at 3712.418 kb on + strand, within bisC at 3712.441 kb on + strand, within bisC at 3712.459 kb on + strand, within bisC at 3712.462 kb on - strand, within bisC at 3712.462 kb on - strand, within bisC at 3712.487 kb on + strand, within bisC at 3712.494 kb on - strand, within bisC at 3712.502 kb on - strand, within bisC at 3712.502 kb on - strand, within bisC at 3712.564 kb on - strand, within bisC at 3712.565 kb on - strand, within bisC at 3712.566 kb on + strand, within bisC at 3712.566 kb on + strand, within bisC at 3712.574 kb on - strand, within bisC at 3712.579 kb on - strand, within bisC at 3712.584 kb on - strand, within bisC at 3712.595 kb on + strand, within bisC at 3712.879 kb on - strand, within bisC at 3712.895 kb on + strand, within bisC at 3712.970 kb on + strand, within bisC at 3712.970 kb on + strand, within bisC at 3712.970 kb on + strand, within bisC at 3713.000 kb on - strand, within bisC at 3713.018 kb on + strand, within bisC at 3713.026 kb on - strand, within bisC at 3713.042 kb on + strand, within bisC at 3713.110 kb on - strand, within bisC at 3713.110 kb on - strand, within bisC at 3713.115 kb on + strand, within bisC at 3713.115 kb on + strand, within bisC
Per-strain Table
Position Strand Gene LocusTag Fraction deuterated glucose in H2O remove 3,710,694 - yhjY b3548 0.62 -1.1 3,710,703 - yhjY b3548 0.64 -0.1 3,710,729 + yhjY b3548 0.67 -0.3 3,710,732 - yhjY b3548 0.68 +2.8 3,710,779 - yhjY b3548 0.74 -1.1 3,710,797 - yhjY b3548 0.77 +0.5 3,710,798 - yhjY b3548 0.77 +0.5 3,710,799 - yhjY b3548 0.77 -0.2 3,710,800 + yhjY b3548 0.77 +1.2 3,710,800 + yhjY b3548 0.77 +0.9 3,710,800 + yhjY b3548 0.77 -0.0 3,710,805 + yhjY b3548 0.78 -0.3 3,710,805 + yhjY b3548 0.78 +0.5 3,710,805 + yhjY b3548 0.78 -0.4 3,710,805 + yhjY b3548 0.78 +1.0 3,710,820 - yhjY b3548 0.80 +0.3 3,710,859 - yhjY b3548 0.86 -0.3 3,710,868 + yhjY b3548 0.87 +1.0 3,710,917 + +1.0 3,710,919 + +0.8 3,710,966 + +0.1 3,710,966 + +1.8 3,710,966 + +0.6 3,710,966 - +0.5 3,710,966 - +0.5 3,710,974 - +0.1 3,711,005 + +1.3 3,711,005 + +1.0 3,711,010 - +0.1 3,711,011 - -0.1 3,711,019 - +1.0 3,711,042 - -0.5 3,711,091 + -1.1 3,711,123 + +0.2 3,711,137 + +1.0 3,711,137 + -1.1 3,711,155 - -0.1 3,711,162 + +0.4 3,711,162 + +0.1 3,711,183 + tag b3549 0.12 -1.8 3,711,183 + tag b3549 0.12 +0.0 3,711,229 + tag b3549 0.20 -0.1 3,711,298 - tag b3549 0.32 -0.6 3,711,298 - +2.8 3,711,352 + tag b3549 0.42 +0.6 3,711,357 - tag b3549 0.43 +2.0 3,711,357 - tag b3549 0.43 -0.4 3,711,360 - tag b3549 0.43 +1.2 3,711,360 - tag b3549 0.43 +0.0 3,711,421 - tag b3549 0.54 -0.2 3,711,433 - tag b3549 0.56 -0.4 3,711,490 - tag b3549 0.66 +0.8 3,711,498 + tag b3549 0.68 -0.8 3,711,500 - tag b3549 0.68 +0.6 3,711,534 - tag b3549 0.74 -0.7 3,711,534 - tag b3549 0.74 +0.5 3,711,613 - tag b3549 0.88 +1.2 3,711,613 - tag b3549 0.88 +0.2 3,711,617 + tag b3549 0.89 -1.0 3,711,617 + tag b3549 0.89 +0.1 3,711,617 + tag b3549 0.89 -2.9 3,711,625 - +0.3 3,711,635 - +0.1 3,711,645 - -0.2 3,711,645 - -0.2 3,711,648 + -1.1 3,711,702 - -2.8 3,711,739 - yiaC b3550 0.15 +0.3 3,711,751 + yiaC b3550 0.17 +0.2 3,711,751 + yiaC b3550 0.17 -0.4 3,711,786 - yiaC b3550 0.25 +1.8 3,711,873 - yiaC b3550 0.45 +1.4 3,711,873 - yiaC b3550 0.45 -1.3 3,711,873 - yiaC b3550 0.45 +0.5 3,711,874 - yiaC b3550 0.45 -2.1 3,711,923 - yiaC b3550 0.56 +0.3 3,711,946 + yiaC b3550 0.61 +0.6 3,711,946 + yiaC b3550 0.61 +1.2 3,711,949 + yiaC b3550 0.62 +0.8 3,711,954 - yiaC b3550 0.63 +0.5 3,711,954 - yiaC b3550 0.63 +1.7 3,711,970 + yiaC b3550 0.67 +0.0 3,711,973 - yiaC b3550 0.68 -0.4 3,712,058 + yiaC b3550 0.87 -0.6 3,712,073 + +0.5 3,712,116 - -0.2 3,712,118 - +0.2 3,712,118 - -0.2 3,712,135 + -1.1 3,712,135 + +1.0 3,712,174 + -0.2 3,712,191 + -1.4 3,712,191 + +0.8 3,712,236 - +0.7 3,712,236 - +0.8 3,712,305 + +0.2 3,712,320 + bisC b3551 0.10 -2.0 3,712,320 + bisC b3551 0.10 +2.8 3,712,349 + bisC b3551 0.11 -0.5 3,712,357 - bisC b3551 0.12 +0.6 3,712,364 + bisC b3551 0.12 +0.6 3,712,364 + bisC b3551 0.12 -0.9 3,712,374 - bisC b3551 0.12 -0.1 3,712,374 - bisC b3551 0.12 +1.0 3,712,382 + bisC b3551 0.13 +0.8 3,712,405 - +1.5 3,712,408 + bisC b3551 0.14 +0.1 3,712,411 - bisC b3551 0.14 -1.0 3,712,418 + bisC b3551 0.14 -2.3 3,712,441 + bisC b3551 0.15 +0.9 3,712,459 + bisC b3551 0.16 -1.7 3,712,462 - bisC b3551 0.16 +0.0 3,712,462 - bisC b3551 0.16 +0.6 3,712,487 + bisC b3551 0.17 +0.8 3,712,494 - bisC b3551 0.18 -1.0 3,712,502 - bisC b3551 0.18 +1.6 3,712,502 - bisC b3551 0.18 -1.3 3,712,564 - bisC b3551 0.21 +0.3 3,712,565 - bisC b3551 0.21 +0.7 3,712,566 + bisC b3551 0.21 +0.5 3,712,566 + bisC b3551 0.21 -1.3 3,712,574 - bisC b3551 0.21 +1.1 3,712,579 - bisC b3551 0.21 -0.3 3,712,584 - bisC b3551 0.21 -0.3 3,712,595 + bisC b3551 0.22 -0.0 3,712,879 - bisC b3551 0.34 +0.6 3,712,895 + bisC b3551 0.35 +0.7 3,712,970 + bisC b3551 0.38 +0.8 3,712,970 + bisC b3551 0.38 +0.9 3,712,970 + bisC b3551 0.38 +0.8 3,713,000 - bisC b3551 0.39 -1.0 3,713,018 + bisC b3551 0.40 +0.7 3,713,026 - bisC b3551 0.40 +0.6 3,713,042 + bisC b3551 0.41 -0.6 3,713,110 - bisC b3551 0.44 +0.4 3,713,110 - bisC b3551 0.44 +0.0 3,713,115 + bisC b3551 0.44 +0.2 3,713,115 + bisC b3551 0.44 +0.5
Or see this region's nucleotide sequence