Experiment: deuterated glucose in H2O
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdxB and flk are separated by 98 nucleotides flk and yfcJ overlap by 4 nucleotides
b2320: pdxB - erythronate-4-phosphate dehydrogenase (NCBI), at 2,434,737 to 2,435,873
pdxB
b2321: flk - predicted flagella assembly protein (NCBI), at 2,435,972 to 2,436,967
flk
b2322: yfcJ - predicted transporter (NCBI), at 2,436,964 to 2,438,142
yfcJ
Position (kb)
2435
2436
2437 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2434.999 kb on - strand, within pdxB at 2435.038 kb on - strand, within pdxB at 2435.105 kb on - strand at 2435.110 kb on + strand, within pdxB at 2435.110 kb on + strand, within pdxB at 2435.230 kb on + strand, within pdxB at 2435.230 kb on + strand, within pdxB at 2435.246 kb on + strand, within pdxB at 2435.285 kb on + strand, within pdxB at 2435.324 kb on + strand, within pdxB at 2435.324 kb on + strand, within pdxB at 2435.343 kb on - strand, within pdxB at 2435.511 kb on + strand, within pdxB at 2435.796 kb on - strand at 2435.796 kb on - strand at 2435.801 kb on - strand at 2435.838 kb on + strand at 2435.838 kb on + strand at 2435.913 kb on - strand at 2435.939 kb on - strand at 2435.982 kb on - strand at 2436.015 kb on - strand at 2436.067 kb on + strand at 2436.124 kb on - strand, within flk at 2436.134 kb on - strand, within flk at 2436.134 kb on - strand, within flk at 2436.134 kb on - strand, within flk at 2436.164 kb on - strand, within flk at 2436.211 kb on + strand, within flk at 2436.258 kb on - strand, within flk at 2436.258 kb on - strand, within flk at 2436.260 kb on + strand, within flk at 2436.352 kb on - strand, within flk at 2436.352 kb on - strand, within flk at 2436.359 kb on + strand, within flk at 2436.359 kb on + strand, within flk at 2436.629 kb on - strand, within flk at 2436.774 kb on + strand, within flk at 2436.813 kb on - strand, within flk at 2436.813 kb on - strand, within flk at 2436.828 kb on + strand, within flk at 2436.914 kb on - strand at 2436.923 kb on + strand at 2436.923 kb on + strand at 2436.927 kb on - strand at 2436.933 kb on + strand at 2436.941 kb on - strand at 2436.947 kb on - strand at 2436.954 kb on + strand at 2436.993 kb on + strand at 2437.001 kb on - strand at 2437.001 kb on - strand at 2437.008 kb on + strand at 2437.207 kb on + strand, within yfcJ at 2437.207 kb on + strand, within yfcJ at 2437.285 kb on + strand, within yfcJ at 2437.287 kb on - strand, within yfcJ at 2437.329 kb on - strand, within yfcJ at 2437.389 kb on - strand, within yfcJ at 2437.446 kb on - strand, within yfcJ at 2437.471 kb on + strand, within yfcJ at 2437.534 kb on + strand, within yfcJ at 2437.574 kb on + strand, within yfcJ at 2437.687 kb on - strand, within yfcJ at 2437.818 kb on + strand, within yfcJ at 2437.818 kb on + strand, within yfcJ at 2437.818 kb on + strand, within yfcJ at 2437.917 kb on - strand, within yfcJ
Per-strain Table
Position Strand Gene LocusTag Fraction deuterated glucose in H2O remove 2,434,999 - pdxB b2320 0.23 -4.0 2,435,038 - pdxB b2320 0.26 -2.0 2,435,105 - +2.3 2,435,110 + pdxB b2320 0.33 -2.6 2,435,110 + pdxB b2320 0.33 -1.8 2,435,230 + pdxB b2320 0.43 -2.2 2,435,230 + pdxB b2320 0.43 -1.4 2,435,246 + pdxB b2320 0.45 -3.4 2,435,285 + pdxB b2320 0.48 -2.7 2,435,324 + pdxB b2320 0.52 -4.0 2,435,324 + pdxB b2320 0.52 -2.8 2,435,343 - pdxB b2320 0.53 -3.7 2,435,511 + pdxB b2320 0.68 +0.3 2,435,796 - -3.3 2,435,796 - -0.4 2,435,801 - -2.8 2,435,838 + -1.9 2,435,838 + -2.9 2,435,913 - -1.9 2,435,939 - -0.5 2,435,982 - +0.5 2,436,015 - -1.1 2,436,067 + +0.6 2,436,124 - flk b2321 0.15 +0.2 2,436,134 - flk b2321 0.16 +0.9 2,436,134 - flk b2321 0.16 -0.2 2,436,134 - flk b2321 0.16 +0.0 2,436,164 - flk b2321 0.19 -0.8 2,436,211 + flk b2321 0.24 -1.7 2,436,258 - flk b2321 0.29 -1.2 2,436,258 - flk b2321 0.29 -2.6 2,436,260 + flk b2321 0.29 +0.3 2,436,352 - flk b2321 0.38 -0.6 2,436,352 - flk b2321 0.38 -1.3 2,436,359 + flk b2321 0.39 -0.1 2,436,359 + flk b2321 0.39 +0.7 2,436,629 - flk b2321 0.66 -0.6 2,436,774 + flk b2321 0.81 -0.1 2,436,813 - flk b2321 0.84 -0.3 2,436,813 - flk b2321 0.84 -0.7 2,436,828 + flk b2321 0.86 +0.3 2,436,914 - +1.3 2,436,923 + +0.3 2,436,923 + +0.0 2,436,927 - +0.3 2,436,933 + -0.4 2,436,941 - +0.4 2,436,947 - +0.6 2,436,954 + -0.0 2,436,993 + +1.0 2,437,001 - +1.8 2,437,001 - +0.6 2,437,008 + -0.3 2,437,207 + yfcJ b2322 0.21 +1.0 2,437,207 + yfcJ b2322 0.21 -0.7 2,437,285 + yfcJ b2322 0.27 -0.3 2,437,287 - yfcJ b2322 0.27 -0.4 2,437,329 - yfcJ b2322 0.31 +0.2 2,437,389 - yfcJ b2322 0.36 +0.7 2,437,446 - yfcJ b2322 0.41 +0.6 2,437,471 + yfcJ b2322 0.43 -1.1 2,437,534 + yfcJ b2322 0.48 +0.9 2,437,574 + yfcJ b2322 0.52 -2.3 2,437,687 - yfcJ b2322 0.61 +0.2 2,437,818 + yfcJ b2322 0.72 -0.8 2,437,818 + yfcJ b2322 0.72 -0.3 2,437,818 + yfcJ b2322 0.72 -0.1 2,437,917 - yfcJ b2322 0.81 +0.4
Or see this region's nucleotide sequence