Experiment: deuterated glucose in H2O
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt atoB and yfaP are separated by 73 nucleotides yfaP and yfaQ are separated by 4 nucleotides
b2224: atoB - acetyl-CoA acetyltransferase (NCBI), at 2,324,131 to 2,325,315
atoB
b2225: yfaP - hypothetical protein (NCBI), at 2,325,389 to 2,326,165
yfaP
b2226: yfaQ - hypothetical protein (NCBI), at 2,326,170 to 2,327,819
yfaQ
Position (kb)
2325
2326
2327 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2324.469 kb on + strand, within atoB at 2324.539 kb on - strand, within atoB at 2324.547 kb on + strand, within atoB at 2324.547 kb on + strand, within atoB at 2324.549 kb on + strand, within atoB at 2324.585 kb on - strand, within atoB at 2324.735 kb on + strand, within atoB at 2324.886 kb on - strand, within atoB at 2325.002 kb on - strand, within atoB at 2325.006 kb on - strand, within atoB at 2325.077 kb on - strand, within atoB at 2325.094 kb on + strand, within atoB at 2325.215 kb on - strand at 2325.222 kb on - strand at 2325.224 kb on - strand at 2325.224 kb on - strand at 2325.226 kb on + strand at 2325.354 kb on + strand at 2325.354 kb on + strand at 2325.393 kb on - strand at 2325.393 kb on - strand at 2325.488 kb on + strand, within yfaP at 2325.526 kb on + strand, within yfaP at 2325.537 kb on - strand, within yfaP at 2325.555 kb on - strand, within yfaP at 2325.623 kb on - strand, within yfaP at 2325.623 kb on - strand, within yfaP at 2325.656 kb on - strand, within yfaP at 2325.756 kb on + strand, within yfaP at 2325.759 kb on - strand, within yfaP at 2325.970 kb on + strand, within yfaP at 2325.970 kb on + strand, within yfaP at 2326.112 kb on - strand at 2326.112 kb on - strand at 2326.209 kb on + strand at 2326.209 kb on + strand at 2326.237 kb on - strand at 2326.286 kb on - strand at 2326.338 kb on - strand, within yfaQ at 2326.388 kb on - strand, within yfaQ at 2326.388 kb on - strand, within yfaQ at 2326.393 kb on - strand, within yfaQ at 2326.394 kb on - strand, within yfaQ at 2326.397 kb on - strand, within yfaQ at 2326.490 kb on + strand, within yfaQ at 2326.531 kb on + strand, within yfaQ at 2326.531 kb on + strand, within yfaQ at 2326.551 kb on + strand, within yfaQ at 2326.584 kb on + strand, within yfaQ at 2326.675 kb on + strand, within yfaQ at 2326.690 kb on + strand, within yfaQ at 2326.803 kb on - strand, within yfaQ at 2326.803 kb on - strand, within yfaQ at 2326.893 kb on + strand at 2326.954 kb on + strand, within yfaQ at 2326.954 kb on + strand, within yfaQ at 2326.954 kb on + strand, within yfaQ at 2327.035 kb on + strand, within yfaQ at 2327.053 kb on - strand, within yfaQ at 2327.053 kb on - strand, within yfaQ at 2327.135 kb on + strand, within yfaQ at 2327.135 kb on + strand, within yfaQ
Per-strain Table
Position Strand Gene LocusTag Fraction deuterated glucose in H2O remove 2,324,469 + atoB b2224 0.29 +1.1 2,324,539 - atoB b2224 0.34 -0.2 2,324,547 + atoB b2224 0.35 +0.8 2,324,547 + atoB b2224 0.35 +0.4 2,324,549 + atoB b2224 0.35 -0.2 2,324,585 - atoB b2224 0.38 +0.7 2,324,735 + atoB b2224 0.51 -0.3 2,324,886 - atoB b2224 0.64 -0.7 2,325,002 - atoB b2224 0.74 -1.1 2,325,006 - atoB b2224 0.74 +0.1 2,325,077 - atoB b2224 0.80 -0.1 2,325,094 + atoB b2224 0.81 -0.1 2,325,215 - +0.5 2,325,222 - -1.6 2,325,224 - +0.7 2,325,224 - +1.1 2,325,226 + +0.1 2,325,354 + +0.3 2,325,354 + -1.0 2,325,393 - -0.1 2,325,393 - -0.0 2,325,488 + yfaP b2225 0.13 +0.8 2,325,526 + yfaP b2225 0.18 +0.5 2,325,537 - yfaP b2225 0.19 +1.3 2,325,555 - yfaP b2225 0.21 +2.7 2,325,623 - yfaP b2225 0.30 +1.0 2,325,623 - yfaP b2225 0.30 +0.4 2,325,656 - yfaP b2225 0.34 +0.3 2,325,756 + yfaP b2225 0.47 +0.1 2,325,759 - yfaP b2225 0.48 +0.8 2,325,970 + yfaP b2225 0.75 +0.3 2,325,970 + yfaP b2225 0.75 +0.1 2,326,112 - +0.1 2,326,112 - +0.4 2,326,209 + +0.0 2,326,209 + +1.2 2,326,237 - +1.1 2,326,286 - -0.8 2,326,338 - yfaQ b2226 0.10 -0.2 2,326,388 - yfaQ b2226 0.13 +1.3 2,326,388 - yfaQ b2226 0.13 -0.5 2,326,393 - yfaQ b2226 0.14 -0.2 2,326,394 - yfaQ b2226 0.14 -0.3 2,326,397 - yfaQ b2226 0.14 -0.4 2,326,490 + yfaQ b2226 0.19 +0.1 2,326,531 + yfaQ b2226 0.22 +0.5 2,326,531 + yfaQ b2226 0.22 -1.5 2,326,551 + yfaQ b2226 0.23 -2.2 2,326,584 + yfaQ b2226 0.25 -3.2 2,326,675 + yfaQ b2226 0.31 -1.4 2,326,690 + yfaQ b2226 0.32 +0.1 2,326,803 - yfaQ b2226 0.38 -0.3 2,326,803 - yfaQ b2226 0.38 -1.8 2,326,893 + +2.3 2,326,954 + yfaQ b2226 0.48 -0.6 2,326,954 + yfaQ b2226 0.48 +0.9 2,326,954 + yfaQ b2226 0.48 -0.0 2,327,035 + yfaQ b2226 0.52 -0.0 2,327,053 - yfaQ b2226 0.54 -0.3 2,327,053 - yfaQ b2226 0.54 +0.1 2,327,135 + yfaQ b2226 0.58 -0.7 2,327,135 + yfaQ b2226 0.58 -0.5
Or see this region's nucleotide sequence