Strain Fitness in Escherichia coli BW25113 around b1432

Experiment: deuterated glucose in H2O

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydcL and insP are separated by 92 nucleotidesinsP and insQ are separated by 67 nucleotidesinsQ and b1433 are separated by 39 nucleotides b1431: ydcL - predicted lipoprotein (NCBI), at 1,500,481 to 1,501,149 ydcL b4578: insP - no description, at 1,501,242 to 1,501,673 insP b1432: insQ - IS609 transposase B (RefSeq), at 1,501,741 to 1,502,889 insQ b1433: b1433 - putative membrane transport protein (VIMSS), at 1,502,929 to 1,504,104 b1433 Position (kb) 1501 1502 1503Strain fitness (log2 ratio) -2 -1 0 1 2at 1500.762 kb on + strand, within ydcLat 1500.946 kb on + strand, within ydcLat 1501.107 kb on + strandat 1501.107 kb on + strandat 1501.126 kb on - strandat 1501.226 kb on - strandat 1501.226 kb on - strandat 1501.257 kb on + strandat 1501.305 kb on - strand, within insPat 1501.305 kb on - strand, within insPat 1501.361 kb on - strand, within insPat 1501.430 kb on - strand, within insPat 1501.472 kb on - strand, within insPat 1501.681 kb on + strandat 1501.681 kb on + strandat 1501.929 kb on + strand, within insQat 1502.154 kb on + strand, within insQat 1502.160 kb on + strand, within insQat 1502.263 kb on + strand, within insQat 1502.469 kb on + strand, within insQat 1502.664 kb on + strand, within insQat 1502.664 kb on + strand, within insQat 1502.809 kb on + strandat 1502.835 kb on - strandat 1503.340 kb on - strand, within b1433at 1503.447 kb on + strand, within b1433at 1503.447 kb on + strand, within b1433at 1503.479 kb on - strand, within b1433at 1503.479 kb on - strand, within b1433at 1503.536 kb on + strand, within b1433at 1503.536 kb on + strand, within b1433at 1503.832 kb on + strand, within b1433

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Per-strain Table

Position Strand Gene LocusTag Fraction deuterated glucose in H2O
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1,500,762 + ydcL b1431 0.42 -0.3
1,500,946 + ydcL b1431 0.70 -0.9
1,501,107 + -2.6
1,501,107 + -1.4
1,501,126 - +0.1
1,501,226 - +0.8
1,501,226 - -0.2
1,501,257 + -0.1
1,501,305 - insP b4578 0.15 +0.6
1,501,305 - insP b4578 0.15 +0.3
1,501,361 - insP b4578 0.28 +0.4
1,501,430 - insP b4578 0.44 +0.2
1,501,472 - insP b4578 0.53 +0.3
1,501,681 + +0.5
1,501,681 + +1.2
1,501,929 + insQ b1432 0.16 -0.9
1,502,154 + insQ b1432 0.36 +2.1
1,502,160 + insQ b1432 0.36 -0.7
1,502,263 + insQ b1432 0.45 -1.1
1,502,469 + insQ b1432 0.63 -1.0
1,502,664 + insQ b1432 0.80 -1.2
1,502,664 + insQ b1432 0.80 -1.1
1,502,809 + -1.2
1,502,835 - +1.1
1,503,340 - b1433 b1433 0.35 +0.1
1,503,447 + b1433 b1433 0.44 +0.8
1,503,447 + b1433 b1433 0.44 +0.4
1,503,479 - b1433 b1433 0.47 +0.1
1,503,479 - b1433 b1433 0.47 +0.3
1,503,536 + b1433 b1433 0.52 -0.6
1,503,536 + b1433 b1433 0.52 +0.5
1,503,832 + b1433 b1433 0.77 -0.1

Or see this region's nucleotide sequence