Experiment: deuterated glucose in H2O
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rluB and btuR are separated by 39 nucleotides btuR and yciK overlap by 4 nucleotides yciK and sohB are separated by 219 nucleotides
b1269: rluB - 23S rRNA pseudouridylate synthase (NCBI), at 1,324,876 to 1,325,751
rluB
b1270: btuR - cob(I)yrinic acid a,c-diamide adenosyltransferase (NCBI), at 1,325,791 to 1,326,381
btuR
b1271: yciK - short chain dehydrogenase (NCBI), at 1,326,378 to 1,327,136
yciK
b1272: sohB - predicted inner membrane peptidase (NCBI), at 1,327,356 to 1,328,405
sohB
Position (kb)
1326
1327
1328 Strain fitness (log2 ratio)
-1
0
1 at 1325.635 kb on - strand, within rluB at 1325.636 kb on - strand, within rluB at 1325.636 kb on - strand, within rluB at 1325.814 kb on + strand at 1325.882 kb on - strand, within btuR at 1325.954 kb on - strand, within btuR at 1326.099 kb on - strand, within btuR at 1326.099 kb on - strand, within btuR at 1326.296 kb on - strand, within btuR at 1326.420 kb on + strand at 1326.420 kb on + strand at 1326.546 kb on + strand, within yciK at 1326.719 kb on + strand, within yciK at 1326.742 kb on - strand, within yciK at 1326.851 kb on - strand, within yciK at 1327.275 kb on + strand at 1327.297 kb on + strand at 1327.500 kb on + strand, within sohB at 1327.505 kb on + strand, within sohB at 1327.505 kb on + strand, within sohB at 1327.511 kb on - strand, within sohB at 1327.511 kb on - strand, within sohB at 1327.565 kb on + strand, within sohB at 1327.565 kb on + strand, within sohB at 1327.752 kb on - strand, within sohB at 1327.752 kb on - strand, within sohB at 1327.797 kb on + strand, within sohB at 1327.797 kb on + strand, within sohB at 1327.902 kb on - strand, within sohB at 1328.003 kb on + strand, within sohB at 1328.032 kb on - strand, within sohB at 1328.039 kb on + strand, within sohB at 1328.039 kb on + strand, within sohB
Per-strain Table
Position Strand Gene LocusTag Fraction deuterated glucose in H2O remove 1,325,635 - rluB b1269 0.87 -0.7 1,325,636 - rluB b1269 0.87 -1.0 1,325,636 - rluB b1269 0.87 -0.8 1,325,814 + +0.8 1,325,882 - btuR b1270 0.15 -1.0 1,325,954 - btuR b1270 0.28 +0.2 1,326,099 - btuR b1270 0.52 -0.7 1,326,099 - btuR b1270 0.52 -1.5 1,326,296 - btuR b1270 0.85 -1.7 1,326,420 + +1.1 1,326,420 + +1.2 1,326,546 + yciK b1271 0.22 -0.2 1,326,719 + yciK b1271 0.45 -0.4 1,326,742 - yciK b1271 0.48 -1.3 1,326,851 - yciK b1271 0.62 -0.7 1,327,275 + +0.2 1,327,297 + -0.9 1,327,500 + sohB b1272 0.14 -0.4 1,327,505 + sohB b1272 0.14 +0.2 1,327,505 + sohB b1272 0.14 +0.8 1,327,511 - sohB b1272 0.15 +0.4 1,327,511 - sohB b1272 0.15 -0.4 1,327,565 + sohB b1272 0.20 +0.5 1,327,565 + sohB b1272 0.20 +0.4 1,327,752 - sohB b1272 0.38 -0.2 1,327,752 - sohB b1272 0.38 -0.8 1,327,797 + sohB b1272 0.42 +1.6 1,327,797 + sohB b1272 0.42 +0.6 1,327,902 - sohB b1272 0.52 +1.1 1,328,003 + sohB b1272 0.62 +1.4 1,328,032 - sohB b1272 0.64 +0.7 1,328,039 + sohB b1272 0.65 -0.3 1,328,039 + sohB b1272 0.65 -0.4
Or see this region's nucleotide sequence