Experiment: deuterated glucose in H2O
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tesA and ybbA overlap by 33 nucleotides ybbA and ybbP overlap by 4 nucleotides ybbP and rhsD are separated by 430 nucleotides
b0494: tesA - multifunctional acyl-CoA thioesterase I and protease I and lysophospholipase L1 (NCBI), at 518,363 to 518,989
tesA
b0495: ybbA - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 518,957 to 519,643
ybbA
b0496: ybbP - predicted inner membrane protein (NCBI), at 519,640 to 522,054
ybbP
b0497: rhsD - rhsD element protein (NCBI), at 522,485 to 526,765
rhsD
Position (kb)
519
520
521
522
523 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 518.768 kb on + strand, within tesA at 518.839 kb on - strand, within tesA at 518.839 kb on - strand, within tesA at 518.839 kb on - strand, within tesA at 519.006 kb on + strand at 519.102 kb on + strand, within ybbA at 519.181 kb on - strand, within ybbA at 519.306 kb on - strand, within ybbA at 519.306 kb on - strand, within ybbA at 519.353 kb on + strand, within ybbA at 519.353 kb on + strand at 519.354 kb on + strand, within ybbA at 519.407 kb on + strand, within ybbA at 519.407 kb on + strand, within ybbA at 519.534 kb on + strand, within ybbA at 519.707 kb on + strand at 519.770 kb on - strand at 519.770 kb on - strand at 519.908 kb on - strand, within ybbP at 519.913 kb on - strand, within ybbP at 519.981 kb on - strand, within ybbP at 520.140 kb on + strand, within ybbP at 520.269 kb on - strand, within ybbP at 520.269 kb on - strand, within ybbP at 520.316 kb on - strand, within ybbP at 520.816 kb on - strand, within ybbP at 520.848 kb on + strand, within ybbP at 520.873 kb on + strand, within ybbP at 520.873 kb on + strand, within ybbP at 520.903 kb on + strand, within ybbP at 521.035 kb on - strand, within ybbP at 521.066 kb on + strand, within ybbP at 521.140 kb on - strand, within ybbP at 521.145 kb on - strand, within ybbP at 521.151 kb on + strand, within ybbP at 521.193 kb on + strand, within ybbP at 521.263 kb on + strand, within ybbP at 521.263 kb on + strand, within ybbP at 521.363 kb on - strand, within ybbP at 521.364 kb on + strand, within ybbP at 521.385 kb on - strand, within ybbP at 521.385 kb on - strand, within ybbP at 521.416 kb on + strand, within ybbP at 521.470 kb on - strand, within ybbP at 521.478 kb on - strand, within ybbP at 521.609 kb on + strand, within ybbP at 521.659 kb on - strand, within ybbP at 521.698 kb on - strand, within ybbP at 521.736 kb on + strand, within ybbP at 521.750 kb on - strand, within ybbP at 522.015 kb on + strand at 522.015 kb on + strand at 522.015 kb on + strand at 522.033 kb on + strand at 522.033 kb on + strand at 522.033 kb on + strand at 522.049 kb on + strand at 522.049 kb on + strand at 522.050 kb on + strand at 522.053 kb on + strand at 522.053 kb on + strand at 522.053 kb on + strand at 522.058 kb on + strand at 522.074 kb on + strand at 522.074 kb on + strand at 522.109 kb on + strand at 522.109 kb on + strand at 522.462 kb on - strand at 522.462 kb on - strand at 522.487 kb on - strand at 522.508 kb on + strand at 522.508 kb on + strand at 522.518 kb on - strand at 522.522 kb on - strand at 522.522 kb on - strand at 522.532 kb on - strand at 522.534 kb on - strand at 522.534 kb on - strand at 522.545 kb on - strand at 522.565 kb on - strand at 522.585 kb on - strand at 522.585 kb on - strand at 522.589 kb on - strand at 522.589 kb on - strand at 522.600 kb on + strand at 522.653 kb on + strand at 522.661 kb on + strand at 522.661 kb on + strand at 522.811 kb on - strand at 522.891 kb on - strand at 522.906 kb on - strand at 522.921 kb on + strand, within rhsD at 522.998 kb on - strand, within rhsD at 522.998 kb on - strand, within rhsD at 523.030 kb on + strand, within rhsD at 523.045 kb on - strand, within rhsD
Per-strain Table
Position Strand Gene LocusTag Fraction deuterated glucose in H2O remove 518,768 + tesA b0494 0.65 +0.4 518,839 - tesA b0494 0.76 -0.1 518,839 - tesA b0494 0.76 +2.3 518,839 - tesA b0494 0.76 +1.4 519,006 + +0.7 519,102 + ybbA b0495 0.21 -0.2 519,181 - ybbA b0495 0.33 +1.1 519,306 - ybbA b0495 0.51 +1.4 519,306 - ybbA b0495 0.51 -0.7 519,353 + ybbA b0495 0.58 +2.4 519,353 + +2.4 519,354 + ybbA b0495 0.58 +1.4 519,407 + ybbA b0495 0.66 +1.6 519,407 + ybbA b0495 0.66 +1.6 519,534 + ybbA b0495 0.84 -0.4 519,707 + -0.2 519,770 - +1.0 519,770 - -0.6 519,908 - ybbP b0496 0.11 +1.1 519,913 - ybbP b0496 0.11 +2.0 519,981 - ybbP b0496 0.14 +1.5 520,140 + ybbP b0496 0.21 +0.1 520,269 - ybbP b0496 0.26 +1.4 520,269 - ybbP b0496 0.26 +2.4 520,316 - ybbP b0496 0.28 +0.6 520,816 - ybbP b0496 0.49 +2.4 520,848 + ybbP b0496 0.50 +2.4 520,873 + ybbP b0496 0.51 +2.8 520,873 + ybbP b0496 0.51 +1.1 520,903 + ybbP b0496 0.52 +0.8 521,035 - ybbP b0496 0.58 +1.8 521,066 + ybbP b0496 0.59 +1.6 521,140 - ybbP b0496 0.62 +2.2 521,145 - ybbP b0496 0.62 +1.5 521,151 + ybbP b0496 0.63 +1.6 521,193 + ybbP b0496 0.64 +0.1 521,263 + ybbP b0496 0.67 +1.6 521,263 + ybbP b0496 0.67 +1.2 521,363 - ybbP b0496 0.71 +2.5 521,364 + ybbP b0496 0.71 +1.4 521,385 - ybbP b0496 0.72 +1.9 521,385 - ybbP b0496 0.72 -0.2 521,416 + ybbP b0496 0.74 +1.4 521,470 - ybbP b0496 0.76 -1.1 521,478 - ybbP b0496 0.76 +1.2 521,609 + ybbP b0496 0.82 +0.9 521,659 - ybbP b0496 0.84 +1.9 521,698 - ybbP b0496 0.85 +1.3 521,736 + ybbP b0496 0.87 +1.6 521,750 - ybbP b0496 0.87 +1.4 522,015 + +1.1 522,015 + +1.8 522,015 + +1.4 522,033 + +1.2 522,033 + +1.1 522,033 + -0.1 522,049 + +0.8 522,049 + +0.1 522,050 + +2.4 522,053 + +1.9 522,053 + +1.4 522,053 + +1.0 522,058 + -0.2 522,074 + -0.3 522,074 + -0.4 522,109 + +1.4 522,109 + +1.1 522,462 - +1.3 522,462 - +1.7 522,487 - -0.5 522,508 + +0.8 522,508 + +2.9 522,518 - +2.1 522,522 - +1.8 522,522 - +1.6 522,532 - +1.1 522,534 - -0.0 522,534 - +2.1 522,545 - +1.4 522,565 - +1.5 522,585 - +2.5 522,585 - +1.2 522,589 - +1.7 522,589 - +2.6 522,600 + +0.5 522,653 + +0.8 522,661 + +4.0 522,661 + +2.1 522,811 - -1.2 522,891 - +3.0 522,906 - +0.5 522,921 + rhsD b0497 0.10 +0.6 522,998 - rhsD b0497 0.12 +1.4 522,998 - rhsD b0497 0.12 +1.3 523,030 + rhsD b0497 0.13 +1.8 523,045 - rhsD b0497 0.13 -0.5
Or see this region's nucleotide sequence