Experiment: deuterated glucose in H2O
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt insA-2 and insX are separated by 238 nucleotides insX and yagB are separated by 64 nucleotides yagB and yagA are separated by 93 nucleotides yagA and yagE are separated by 294 nucleotides
b0265: insA-2 - CP4-6 prophage; IS1 repressor protein InsA (NCBI), at 278,824 to 279,099
insA-2
b4505: insX - predicted IS protein (RefSeq), at 279,338 to 279,586
insX
b0266: yagB - orf, hypothetical protein (VIMSS), at 279,651 to 279,959
yagB
b0267: yagA - CP4-6 prophage; predicted DNA-binding transcriptional regulator (NCBI), at 280,053 to 281,207
yagA
b0268: yagE - 2-keto-3-deoxy gluconate (KDG) aldolase; CP4-6 prophage (NCBI), at 281,502 to 282,410
yagE
Position (kb) 280
281
282 Strain fitness (log2 ratio) -1
0
1
2 at 279.472 kb on - strand, within insX at 279.476 kb on - strand, within insX at 279.573 kb on + strand at 279.581 kb on - strand at 279.581 kb on - strand at 279.584 kb on + strand at 279.584 kb on + strand at 279.624 kb on - strand at 279.624 kb on - strand at 279.714 kb on + strand, within yagB at 279.719 kb on + strand, within yagB at 279.719 kb on + strand, within yagB at 279.719 kb on + strand, within yagB at 279.721 kb on + strand, within yagB at 279.721 kb on + strand, within yagB at 279.727 kb on - strand, within yagB at 279.729 kb on - strand, within yagB at 279.729 kb on - strand, within yagB at 279.747 kb on - strand, within yagB at 279.757 kb on - strand, within yagB at 279.824 kb on - strand, within yagB at 279.829 kb on - strand, within yagB at 279.857 kb on - strand, within yagB at 279.882 kb on + strand, within yagB at 279.882 kb on + strand, within yagB at 279.890 kb on - strand, within yagB at 279.890 kb on - strand, within yagB at 279.909 kb on + strand, within yagB at 279.936 kb on + strand at 280.030 kb on + strand at 280.060 kb on + strand at 280.106 kb on + strand at 280.106 kb on + strand at 280.122 kb on - strand at 280.122 kb on - strand at 280.127 kb on + strand at 280.172 kb on - strand, within yagA at 280.182 kb on - strand, within yagA at 280.184 kb on + strand, within yagA at 280.184 kb on + strand, within yagA at 280.288 kb on + strand, within yagA at 280.288 kb on + strand, within yagA at 280.362 kb on - strand, within yagA at 280.362 kb on - strand, within yagA at 280.370 kb on - strand, within yagA at 280.390 kb on - strand, within yagA at 280.395 kb on + strand, within yagA at 280.395 kb on - strand, within yagA at 280.395 kb on - strand, within yagA at 280.398 kb on - strand, within yagA at 280.581 kb on + strand, within yagA at 280.715 kb on + strand, within yagA at 280.940 kb on + strand, within yagA at 280.955 kb on + strand, within yagA at 280.955 kb on + strand, within yagA at 280.973 kb on - strand, within yagA at 281.054 kb on - strand, within yagA at 281.054 kb on - strand, within yagA at 281.236 kb on + strand at 281.236 kb on + strand at 281.271 kb on + strand at 281.293 kb on - strand at 281.293 kb on - strand at 281.359 kb on - strand at 281.359 kb on - strand at 281.464 kb on + strand at 281.464 kb on + strand at 281.579 kb on + strand at 281.579 kb on + strand at 281.639 kb on - strand, within yagE at 281.664 kb on + strand, within yagE at 281.869 kb on + strand, within yagE at 282.021 kb on - strand, within yagE at 282.021 kb on - strand, within yagE at 282.072 kb on + strand, within yagE at 282.101 kb on + strand, within yagE at 282.109 kb on - strand, within yagE at 282.109 kb on - strand, within yagE at 282.114 kb on - strand, within yagE at 282.114 kb on - strand, within yagE
Per-strain Table
Position Strand Gene LocusTag Fraction deuterated glucose in H2O remove 279,472 - insX b4505 0.54 +2.5 279,476 - insX b4505 0.55 +0.5 279,573 + +0.9 279,581 - -0.1 279,581 - +0.5 279,584 + +0.9 279,584 + +0.9 279,624 - -0.8 279,624 - -0.7 279,714 + yagB b0266 0.20 -0.3 279,719 + yagB b0266 0.22 +0.5 279,719 + yagB b0266 0.22 -0.1 279,719 + yagB b0266 0.22 +0.4 279,721 + yagB b0266 0.23 +1.1 279,721 + yagB b0266 0.23 -0.9 279,727 - yagB b0266 0.25 +0.5 279,729 - yagB b0266 0.25 +0.5 279,729 - yagB b0266 0.25 +0.2 279,747 - yagB b0266 0.31 +0.9 279,757 - yagB b0266 0.34 +0.1 279,824 - yagB b0266 0.56 +0.3 279,829 - yagB b0266 0.58 -0.5 279,857 - yagB b0266 0.67 +0.3 279,882 + yagB b0266 0.75 +0.6 279,882 + yagB b0266 0.75 +0.2 279,890 - yagB b0266 0.77 +1.2 279,890 - yagB b0266 0.77 +1.5 279,909 + yagB b0266 0.83 +0.6 279,936 + -0.1 280,030 + -1.3 280,060 + -0.1 280,106 + +1.4 280,106 + +2.5 280,122 - +0.9 280,122 - -0.6 280,127 + +0.5 280,172 - yagA b0267 0.10 +0.2 280,182 - yagA b0267 0.11 +2.5 280,184 + yagA b0267 0.11 +0.5 280,184 + yagA b0267 0.11 +0.4 280,288 + yagA b0267 0.20 +0.7 280,288 + yagA b0267 0.20 +2.5 280,362 - yagA b0267 0.27 +0.3 280,362 - yagA b0267 0.27 +0.8 280,370 - yagA b0267 0.27 +0.6 280,390 - yagA b0267 0.29 +0.8 280,395 + yagA b0267 0.30 +1.0 280,395 - yagA b0267 0.30 +0.9 280,395 - yagA b0267 0.30 +0.2 280,398 - yagA b0267 0.30 +0.7 280,581 + yagA b0267 0.46 -0.5 280,715 + yagA b0267 0.57 +1.2 280,940 + yagA b0267 0.77 +0.9 280,955 + yagA b0267 0.78 +0.3 280,955 + yagA b0267 0.78 -0.3 280,973 - yagA b0267 0.80 +1.0 281,054 - yagA b0267 0.87 +0.6 281,054 - yagA b0267 0.87 -0.3 281,236 + +0.5 281,236 + -0.5 281,271 + +1.2 281,293 - +0.5 281,293 - -0.8 281,359 - +0.6 281,359 - +2.5 281,464 + +0.4 281,464 + +2.5 281,579 + -1.1 281,579 + -0.5 281,639 - yagE b0268 0.15 -0.3 281,664 + yagE b0268 0.18 -0.3 281,869 + yagE b0268 0.40 +0.2 282,021 - yagE b0268 0.57 +1.5 282,021 - yagE b0268 0.57 +2.5 282,072 + yagE b0268 0.63 +1.9 282,101 + yagE b0268 0.66 +1.9 282,109 - yagE b0268 0.67 +2.1 282,109 - yagE b0268 0.67 +2.5 282,114 - yagE b0268 0.67 +1.5 282,114 - yagE b0268 0.67 -0.7
Or see this region's nucleotide sequence