Experiment: deuterated glucose in H2O
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt insH-1 and ykfD are separated by 207 nucleotides ykfD and mmuM overlap by 14 nucleotides
b0259: insH-1 - CP4-6 prophage; IS5 transposase and trans-activator (NCBI), at 273,325 to 274,341
insH-1
b0260: ykfD - putative amino acid/amine transport protein (VIMSS), at 274,549 to 275,952
ykfD
b0261: mmuM - homocysteine methyltransferase (NCBI), at 275,939 to 276,871
mmuM
Position (kb)
274
275
276 Strain fitness (log2 ratio)
-1
0
1
2 at 274.371 kb on + strand at 274.376 kb on - strand at 274.376 kb on - strand at 274.428 kb on + strand at 274.461 kb on + strand at 274.497 kb on + strand at 274.712 kb on + strand, within ykfD at 274.725 kb on - strand, within ykfD at 274.725 kb on - strand, within ykfD at 274.781 kb on - strand, within ykfD at 274.781 kb on - strand, within ykfD at 274.790 kb on + strand, within ykfD at 274.791 kb on - strand, within ykfD at 274.851 kb on + strand, within ykfD at 274.855 kb on - strand, within ykfD at 274.908 kb on - strand, within ykfD at 274.913 kb on - strand, within ykfD at 274.932 kb on + strand, within ykfD at 274.950 kb on - strand, within ykfD at 274.950 kb on - strand, within ykfD at 274.955 kb on - strand, within ykfD at 275.190 kb on - strand, within ykfD at 275.516 kb on - strand, within ykfD at 275.585 kb on - strand, within ykfD at 275.627 kb on - strand, within ykfD at 275.883 kb on + strand at 275.883 kb on + strand at 275.976 kb on - strand at 275.986 kb on - strand at 275.988 kb on - strand at 275.988 kb on - strand at 276.000 kb on - strand at 276.059 kb on + strand, within mmuM at 276.073 kb on - strand, within mmuM at 276.073 kb on - strand, within mmuM at 276.333 kb on + strand, within mmuM at 276.346 kb on - strand, within mmuM at 276.346 kb on - strand, within mmuM at 276.358 kb on + strand, within mmuM at 276.415 kb on + strand, within mmuM at 276.580 kb on - strand, within mmuM at 276.621 kb on + strand, within mmuM at 276.621 kb on + strand, within mmuM at 276.669 kb on + strand, within mmuM at 276.677 kb on - strand, within mmuM at 276.682 kb on + strand, within mmuM at 276.685 kb on + strand, within mmuM at 276.882 kb on + strand at 276.890 kb on - strand at 276.890 kb on - strand at 276.890 kb on - strand at 276.890 kb on - strand at 276.931 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction deuterated glucose in H2O remove 274,371 + +2.5 274,376 - +2.1 274,376 - +0.5 274,428 + +1.2 274,461 + +1.5 274,497 + -1.7 274,712 + ykfD b0260 0.12 -0.2 274,725 - ykfD b0260 0.13 +1.5 274,725 - ykfD b0260 0.13 +0.9 274,781 - ykfD b0260 0.17 +1.9 274,781 - ykfD b0260 0.17 +0.6 274,790 + ykfD b0260 0.17 +0.5 274,791 - ykfD b0260 0.17 -0.2 274,851 + ykfD b0260 0.22 -0.5 274,855 - ykfD b0260 0.22 -0.0 274,908 - ykfD b0260 0.26 +0.1 274,913 - ykfD b0260 0.26 +0.8 274,932 + ykfD b0260 0.27 +0.7 274,950 - ykfD b0260 0.29 -0.0 274,950 - ykfD b0260 0.29 +2.5 274,955 - ykfD b0260 0.29 +0.3 275,190 - ykfD b0260 0.46 +0.7 275,516 - ykfD b0260 0.69 +0.8 275,585 - ykfD b0260 0.74 +0.6 275,627 - ykfD b0260 0.77 +1.0 275,883 + +1.0 275,883 + +1.0 275,976 - -0.4 275,986 - +2.0 275,988 - -0.9 275,988 - -0.1 276,000 - +0.7 276,059 + mmuM b0261 0.13 -0.8 276,073 - mmuM b0261 0.14 +0.2 276,073 - mmuM b0261 0.14 +0.4 276,333 + mmuM b0261 0.42 +1.1 276,346 - mmuM b0261 0.44 -0.4 276,346 - mmuM b0261 0.44 +0.3 276,358 + mmuM b0261 0.45 +0.3 276,415 + mmuM b0261 0.51 +0.2 276,580 - mmuM b0261 0.69 -0.0 276,621 + mmuM b0261 0.73 +1.5 276,621 + mmuM b0261 0.73 +1.0 276,669 + mmuM b0261 0.78 +0.6 276,677 - mmuM b0261 0.79 +1.0 276,682 + mmuM b0261 0.80 -1.0 276,685 + mmuM b0261 0.80 -0.7 276,882 + +1.5 276,890 - +1.0 276,890 - +0.5 276,890 - +1.0 276,890 - +0.7 276,931 + +0.0
Or see this region's nucleotide sequence