Strain Fitness in Escherichia coli BW25113 around b0432

Experiment: protonated glucose in D2O

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcyoB and cyoA are separated by 21 nucleotidescyoA and ampG are separated by 459 nucleotides b0431: cyoB - cytochrome o ubiquinol oxidase subunit I (NCBI), at 447,874 to 449,865 cyoB b0432: cyoA - cytochrome o ubiquinol oxidase subunit II (NCBI), at 449,887 to 450,834 cyoA b0433: ampG - muropeptide transporter (NCBI), at 451,294 to 452,769 ampG Position (kb) 449 450 451Strain fitness (log2 ratio) -1 0 1 2at 449.038 kb on + strand, within cyoBat 449.041 kb on - strand, within cyoBat 449.053 kb on - strand, within cyoBat 449.056 kb on - strand, within cyoBat 449.098 kb on + strand, within cyoBat 449.098 kb on + strand, within cyoBat 449.135 kb on + strand, within cyoBat 449.169 kb on + strand, within cyoBat 449.169 kb on + strand, within cyoBat 449.202 kb on + strandat 449.210 kb on - strand, within cyoBat 449.210 kb on - strand, within cyoBat 449.278 kb on + strand, within cyoBat 449.299 kb on + strand, within cyoBat 449.299 kb on + strand, within cyoBat 449.323 kb on + strand, within cyoBat 449.330 kb on + strand, within cyoBat 449.438 kb on - strand, within cyoBat 449.580 kb on - strand, within cyoBat 449.631 kb on + strand, within cyoBat 449.681 kb on + strandat 449.686 kb on - strandat 449.686 kb on - strandat 449.690 kb on + strandat 449.711 kb on + strandat 449.729 kb on + strandat 449.756 kb on + strandat 449.773 kb on - strandat 449.773 kb on - strandat 449.776 kb on + strandat 449.783 kb on - strandat 449.785 kb on + strandat 449.785 kb on + strandat 449.817 kb on + strandat 449.830 kb on + strandat 449.848 kb on - strandat 449.882 kb on + strandat 449.882 kb on + strandat 449.892 kb on - strandat 449.892 kb on - strandat 449.893 kb on + strandat 449.893 kb on + strandat 449.893 kb on + strandat 449.908 kb on + strandat 449.908 kb on + strandat 449.908 kb on + strandat 449.908 kb on + strandat 449.916 kb on - strandat 449.975 kb on + strandat 449.976 kb on - strandat 449.977 kb on - strandat 449.977 kb on - strandat 449.983 kb on - strand, within cyoAat 449.983 kb on - strand, within cyoAat 449.983 kb on - strand, within cyoAat 449.987 kb on + strand, within cyoAat 450.027 kb on - strand, within cyoAat 450.047 kb on + strand, within cyoAat 450.112 kb on - strand, within cyoAat 450.178 kb on - strand, within cyoAat 450.201 kb on - strand, within cyoAat 450.221 kb on - strand, within cyoAat 450.221 kb on - strand, within cyoAat 450.344 kb on + strand, within cyoAat 450.344 kb on + strand, within cyoAat 450.353 kb on + strand, within cyoAat 450.359 kb on - strand, within cyoAat 450.367 kb on - strand, within cyoAat 450.367 kb on - strand, within cyoAat 450.397 kb on + strand, within cyoAat 450.423 kb on - strand, within cyoAat 450.432 kb on - strand, within cyoAat 450.452 kb on - strand, within cyoAat 450.452 kb on - strand, within cyoAat 450.463 kb on + strand, within cyoAat 450.497 kb on - strand, within cyoAat 450.514 kb on + strand, within cyoAat 450.522 kb on + strand, within cyoAat 450.546 kb on - strand, within cyoAat 450.553 kb on + strand, within cyoAat 450.562 kb on - strand, within cyoAat 450.562 kb on - strand, within cyoAat 450.575 kb on + strand, within cyoAat 450.581 kb on - strand, within cyoAat 450.597 kb on + strand, within cyoAat 450.601 kb on + strand, within cyoAat 450.601 kb on - strand, within cyoAat 450.673 kb on - strand, within cyoAat 450.687 kb on + strand, within cyoAat 450.702 kb on + strand, within cyoAat 450.707 kb on + strand, within cyoAat 450.710 kb on - strand, within cyoAat 450.715 kb on - strand, within cyoAat 450.718 kb on + strand, within cyoAat 450.725 kb on - strand, within cyoAat 450.730 kb on - strand, within cyoAat 450.730 kb on - strand, within cyoAat 450.753 kb on - strandat 450.763 kb on - strandat 450.769 kb on + strandat 450.769 kb on + strandat 450.774 kb on + strandat 450.777 kb on - strandat 450.779 kb on - strandat 450.779 kb on - strandat 450.782 kb on - strandat 450.782 kb on - strandat 450.792 kb on + strandat 450.863 kb on - strandat 450.863 kb on - strandat 450.874 kb on + strandat 450.874 kb on - strandat 450.893 kb on - strandat 450.904 kb on - strandat 450.904 kb on - strandat 450.950 kb on + strandat 450.954 kb on + strandat 450.954 kb on + strandat 450.957 kb on - strandat 450.973 kb on - strandat 450.996 kb on + strandat 451.004 kb on - strandat 451.021 kb on - strandat 451.023 kb on + strandat 451.026 kb on + strandat 451.041 kb on - strandat 451.041 kb on - strandat 451.044 kb on - strandat 451.213 kb on + strandat 451.277 kb on - strandat 451.325 kb on - strandat 451.328 kb on - strandat 451.328 kb on - strandat 451.347 kb on - strandat 451.386 kb on - strandat 451.386 kb on - strandat 451.394 kb on - strandat 451.402 kb on + strandat 451.403 kb on - strandat 451.449 kb on + strand, within ampGat 451.449 kb on + strand, within ampGat 451.466 kb on - strand, within ampGat 451.483 kb on - strand, within ampGat 451.590 kb on + strand, within ampGat 451.590 kb on + strand, within ampGat 451.596 kb on - strand, within ampGat 451.596 kb on - strand, within ampGat 451.596 kb on - strand, within ampGat 451.598 kb on - strand, within ampGat 451.630 kb on + strand, within ampGat 451.638 kb on - strand, within ampGat 451.683 kb on - strand, within ampGat 451.700 kb on - strand, within ampGat 451.700 kb on - strand, within ampGat 451.702 kb on - strand, within ampGat 451.742 kb on + strand, within ampGat 451.742 kb on + strand, within ampGat 451.742 kb on + strand, within ampGat 451.776 kb on + strand, within ampGat 451.776 kb on + strand, within ampGat 451.778 kb on - strand, within ampGat 451.809 kb on + strand, within ampGat 451.816 kb on + strand, within ampGat 451.816 kb on + strand, within ampGat 451.817 kb on + strand, within ampGat 451.822 kb on - strand, within ampGat 451.822 kb on - strand, within ampG

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Per-strain Table

Position Strand Gene LocusTag Fraction protonated glucose in D2O
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449,038 + cyoB b0431 0.58 +1.5
449,041 - cyoB b0431 0.59 +0.4
449,053 - cyoB b0431 0.59 +2.5
449,056 - cyoB b0431 0.59 +0.1
449,098 + cyoB b0431 0.61 +0.2
449,098 + cyoB b0431 0.61 +0.9
449,135 + cyoB b0431 0.63 -0.9
449,169 + cyoB b0431 0.65 +1.6
449,169 + cyoB b0431 0.65 +0.6
449,202 + +2.4
449,210 - cyoB b0431 0.67 +0.8
449,210 - cyoB b0431 0.67 +0.8
449,278 + cyoB b0431 0.70 +1.2
449,299 + cyoB b0431 0.72 +1.1
449,299 + cyoB b0431 0.72 +0.2
449,323 + cyoB b0431 0.73 +1.1
449,330 + cyoB b0431 0.73 +0.9
449,438 - cyoB b0431 0.79 +0.3
449,580 - cyoB b0431 0.86 +1.1
449,631 + cyoB b0431 0.88 +0.5
449,681 + -0.6
449,686 - +2.0
449,686 - -0.2
449,690 + +0.6
449,711 + +0.3
449,729 + +0.1
449,756 + +0.6
449,773 - +0.4
449,773 - -0.2
449,776 + +0.4
449,783 - +1.0
449,785 + +0.7
449,785 + -0.0
449,817 + +0.1
449,830 + +0.1
449,848 - +0.7
449,882 + -0.4
449,882 + +0.7
449,892 - +0.5
449,892 - -0.0
449,893 + +0.6
449,893 + +0.2
449,893 + +0.6
449,908 + -0.9
449,908 + +0.9
449,908 + -0.5
449,908 + +0.3
449,916 - +0.3
449,975 + -0.2
449,976 - -0.5
449,977 - -1.4
449,977 - +0.6
449,983 - cyoA b0432 0.10 +0.2
449,983 - cyoA b0432 0.10 +0.4
449,983 - cyoA b0432 0.10 -0.3
449,987 + cyoA b0432 0.11 +0.1
450,027 - cyoA b0432 0.15 -0.4
450,047 + cyoA b0432 0.17 +1.3
450,112 - cyoA b0432 0.24 -0.4
450,178 - cyoA b0432 0.31 +0.7
450,201 - cyoA b0432 0.33 -0.9
450,221 - cyoA b0432 0.35 -1.3
450,221 - cyoA b0432 0.35 +0.9
450,344 + cyoA b0432 0.48 +0.1
450,344 + cyoA b0432 0.48 -1.4
450,353 + cyoA b0432 0.49 +0.5
450,359 - cyoA b0432 0.50 +0.2
450,367 - cyoA b0432 0.51 +0.2
450,367 - cyoA b0432 0.51 -0.1
450,397 + cyoA b0432 0.54 +0.6
450,423 - cyoA b0432 0.57 +0.3
450,432 - cyoA b0432 0.57 +0.4
450,452 - cyoA b0432 0.60 -0.5
450,452 - cyoA b0432 0.60 +0.9
450,463 + cyoA b0432 0.61 +0.8
450,497 - cyoA b0432 0.64 +0.3
450,514 + cyoA b0432 0.66 +0.6
450,522 + cyoA b0432 0.67 +0.6
450,546 - cyoA b0432 0.70 -0.8
450,553 + cyoA b0432 0.70 +0.5
450,562 - cyoA b0432 0.71 +0.6
450,562 - cyoA b0432 0.71 +0.7
450,575 + cyoA b0432 0.73 +0.1
450,581 - cyoA b0432 0.73 +0.7
450,597 + cyoA b0432 0.75 +0.5
450,601 + cyoA b0432 0.75 +0.1
450,601 - cyoA b0432 0.75 +0.3
450,673 - cyoA b0432 0.83 -0.7
450,687 + cyoA b0432 0.84 +0.6
450,702 + cyoA b0432 0.86 +0.3
450,707 + cyoA b0432 0.86 +0.2
450,710 - cyoA b0432 0.87 -0.0
450,715 - cyoA b0432 0.87 -0.1
450,718 + cyoA b0432 0.88 +0.9
450,725 - cyoA b0432 0.88 -0.7
450,730 - cyoA b0432 0.89 +0.8
450,730 - cyoA b0432 0.89 +0.6
450,753 - +0.4
450,763 - -0.2
450,769 + -0.2
450,769 + +0.3
450,774 + +0.5
450,777 - +0.4
450,779 - -0.3
450,779 - -0.9
450,782 - +0.5
450,782 - +1.6
450,792 + -0.5
450,863 - -0.3
450,863 - +0.5
450,874 + +0.4
450,874 - -0.3
450,893 - +0.1
450,904 - +0.6
450,904 - +1.2
450,950 + -1.4
450,954 + +0.3
450,954 + +0.9
450,957 - +0.1
450,973 - -1.2
450,996 + -0.6
451,004 - +1.2
451,021 - -0.2
451,023 + -0.6
451,026 + -1.1
451,041 - -0.2
451,041 - +0.2
451,044 - -0.0
451,213 + -0.6
451,277 - +0.5
451,325 - +0.1
451,328 - -0.5
451,328 - -1.4
451,347 - -1.2
451,386 - -0.7
451,386 - -0.2
451,394 - -1.1
451,402 + +0.2
451,403 - -0.4
451,449 + ampG b0433 0.11 +0.4
451,449 + ampG b0433 0.11 +0.0
451,466 - ampG b0433 0.12 -0.4
451,483 - ampG b0433 0.13 -0.3
451,590 + ampG b0433 0.20 +0.6
451,590 + ampG b0433 0.20 +1.8
451,596 - ampG b0433 0.20 -0.0
451,596 - ampG b0433 0.20 +0.1
451,596 - ampG b0433 0.20 -0.2
451,598 - ampG b0433 0.21 -1.2
451,630 + ampG b0433 0.23 -0.8
451,638 - ampG b0433 0.23 -0.5
451,683 - ampG b0433 0.26 -0.0
451,700 - ampG b0433 0.28 +0.3
451,700 - ampG b0433 0.28 -0.3
451,702 - ampG b0433 0.28 +0.4
451,742 + ampG b0433 0.30 +0.1
451,742 + ampG b0433 0.30 -1.0
451,742 + ampG b0433 0.30 -0.3
451,776 + ampG b0433 0.33 +1.3
451,776 + ampG b0433 0.33 -0.4
451,778 - ampG b0433 0.33 -0.0
451,809 + ampG b0433 0.35 +0.3
451,816 + ampG b0433 0.35 +1.1
451,816 + ampG b0433 0.35 +0.1
451,817 + ampG b0433 0.35 +0.3
451,822 - ampG b0433 0.36 -0.3
451,822 - ampG b0433 0.36 -0.1

Or see this region's nucleotide sequence