Experiment: protonated glucose in D2O
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt malZ and yajB are separated by 4 nucleotides yajB and queA are separated by 92 nucleotides
b0403: malZ - maltodextrin glucosidase (NCBI), at 421,742 to 423,556
malZ
b0404: yajB - hypothetical protein (NCBI), at 423,561 to 424,142
yajB
b0405: queA - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI), at 424,235 to 425,305
queA
Position (kb) 423
424
425 Strain fitness (log2 ratio) -1
0
1 at 422.690 kb on - strand, within malZ at 422.699 kb on + strand, within malZ at 422.745 kb on + strand, within malZ at 422.753 kb on + strand, within malZ at 422.753 kb on + strand, within malZ at 422.795 kb on - strand, within malZ at 422.892 kb on - strand, within malZ at 422.892 kb on - strand, within malZ at 422.905 kb on + strand, within malZ at 422.909 kb on + strand, within malZ at 422.909 kb on + strand, within malZ at 422.968 kb on + strand, within malZ at 423.015 kb on - strand, within malZ at 423.025 kb on - strand, within malZ at 423.025 kb on - strand, within malZ at 423.071 kb on - strand, within malZ at 423.162 kb on + strand, within malZ at 423.283 kb on - strand, within malZ at 423.401 kb on - strand at 423.404 kb on - strand at 423.404 kb on - strand at 423.445 kb on - strand at 423.512 kb on + strand at 423.548 kb on - strand at 423.548 kb on - strand at 423.711 kb on + strand, within yajB at 423.711 kb on + strand, within yajB at 423.909 kb on + strand, within yajB at 423.909 kb on + strand at 423.923 kb on + strand, within yajB at 423.996 kb on + strand, within yajB at 424.011 kb on - strand, within yajB at 424.112 kb on + strand at 424.121 kb on - strand at 424.121 kb on - strand at 424.232 kb on + strand at 424.232 kb on + strand at 424.280 kb on + strand at 424.280 kb on + strand at 424.484 kb on - strand, within queA at 424.484 kb on - strand, within queA at 424.612 kb on + strand, within queA at 424.651 kb on - strand, within queA at 424.651 kb on - strand, within queA at 424.651 kb on - strand, within queA at 424.657 kb on + strand, within queA at 424.668 kb on + strand, within queA at 424.668 kb on + strand, within queA at 424.922 kb on - strand, within queA
Per-strain Table
Position Strand Gene LocusTag Fraction protonated glucose in D2O remove 422,690 - malZ b0403 0.52 -0.1 422,699 + malZ b0403 0.53 +0.4 422,745 + malZ b0403 0.55 +0.8 422,753 + malZ b0403 0.56 -0.0 422,753 + malZ b0403 0.56 +0.2 422,795 - malZ b0403 0.58 +0.0 422,892 - malZ b0403 0.63 +0.2 422,892 - malZ b0403 0.63 +0.6 422,905 + malZ b0403 0.64 +0.4 422,909 + malZ b0403 0.64 +0.4 422,909 + malZ b0403 0.64 +0.2 422,968 + malZ b0403 0.68 +0.1 423,015 - malZ b0403 0.70 +0.0 423,025 - malZ b0403 0.71 +0.3 423,025 - malZ b0403 0.71 +0.0 423,071 - malZ b0403 0.73 -0.7 423,162 + malZ b0403 0.78 +0.3 423,283 - malZ b0403 0.85 +0.2 423,401 - +0.0 423,404 - -1.6 423,404 - -0.6 423,445 - +1.4 423,512 + +1.0 423,548 - +0.7 423,548 - +0.3 423,711 + yajB b0404 0.26 +0.3 423,711 + yajB b0404 0.26 -0.1 423,909 + yajB b0404 0.60 -0.0 423,909 + -0.9 423,923 + yajB b0404 0.62 -0.0 423,996 + yajB b0404 0.75 +0.2 424,011 - yajB b0404 0.77 +1.5 424,112 + +0.9 424,121 - +0.2 424,121 - +0.6 424,232 + -0.4 424,232 + -0.6 424,280 + -0.2 424,280 + -1.3 424,484 - queA b0405 0.23 -0.2 424,484 - queA b0405 0.23 -0.8 424,612 + queA b0405 0.35 +0.1 424,651 - queA b0405 0.39 -0.7 424,651 - queA b0405 0.39 +0.4 424,651 - queA b0405 0.39 +0.8 424,657 + queA b0405 0.39 -1.4 424,668 + queA b0405 0.40 -1.1 424,668 + queA b0405 0.40 +0.2 424,922 - queA b0405 0.64 +0.1
Or see this region's nucleotide sequence