Experiment: protonated glucose in D2O
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yifB and ilvL are separated by 352 nucleotides ilvL and ilvX are separated by 86 nucleotides ilvX and ilvG are separated by 2 nucleotides
b3765: yifB - predicted bifunctional enzyme and transcriptional regulator (RefSeq), at 3,946,472 to 3,947,992
yifB
b3766: ilvL - ilvG operon leader peptide (NCBI), at 3,948,345 to 3,948,443
ilvL
b4669: ilvX - expressed protein (RefSeq), at 3,948,530 to 3,948,580
ilvX
b4488: ilvG - no description, at 3,948,583 to 3,950,227
ilvG
Position (kb)
3948
3949 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3947.409 kb on - strand, within yifB at 3947.423 kb on - strand, within yifB at 3947.423 kb on - strand, within yifB at 3947.423 kb on - strand, within yifB at 3947.429 kb on + strand, within yifB at 3947.429 kb on + strand, within yifB at 3947.437 kb on - strand, within yifB at 3947.449 kb on - strand, within yifB at 3947.449 kb on - strand, within yifB at 3947.479 kb on - strand, within yifB at 3947.481 kb on + strand, within yifB at 3947.506 kb on - strand, within yifB at 3947.506 kb on - strand, within yifB at 3947.523 kb on + strand, within yifB at 3947.523 kb on + strand, within yifB at 3947.532 kb on - strand, within yifB at 3947.543 kb on + strand, within yifB at 3947.543 kb on + strand, within yifB at 3947.746 kb on + strand, within yifB at 3947.833 kb on - strand, within yifB at 3947.836 kb on - strand, within yifB at 3947.841 kb on + strand at 3947.903 kb on + strand at 3947.903 kb on + strand at 3947.984 kb on - strand at 3948.303 kb on + strand at 3948.305 kb on + strand at 3948.364 kb on + strand, within ilvL at 3948.375 kb on + strand, within ilvL at 3948.383 kb on - strand, within ilvL at 3948.410 kb on + strand, within ilvL at 3948.410 kb on + strand, within ilvL at 3948.410 kb on + strand, within ilvL at 3948.531 kb on - strand at 3948.579 kb on + strand at 3948.580 kb on + strand at 3948.580 kb on + strand at 3948.604 kb on + strand at 3948.604 kb on + strand at 3948.620 kb on + strand at 3948.620 kb on + strand at 3948.624 kb on + strand at 3948.633 kb on - strand at 3948.633 kb on - strand at 3948.633 kb on - strand at 3948.682 kb on + strand at 3948.721 kb on + strand at 3948.726 kb on + strand at 3948.748 kb on + strand, within ilvG at 3948.748 kb on + strand, within ilvG at 3948.781 kb on + strand, within ilvG at 3948.815 kb on + strand, within ilvG at 3948.815 kb on + strand, within ilvG at 3948.851 kb on + strand, within ilvG at 3948.851 kb on + strand, within ilvG at 3948.851 kb on + strand, within ilvG at 3948.859 kb on - strand, within ilvG at 3948.933 kb on + strand, within ilvG at 3948.939 kb on + strand, within ilvG at 3948.944 kb on + strand, within ilvG at 3948.947 kb on - strand, within ilvG at 3948.947 kb on - strand, within ilvG at 3948.966 kb on - strand, within ilvG at 3949.037 kb on - strand, within ilvG at 3949.042 kb on - strand, within ilvG at 3949.083 kb on + strand, within ilvG at 3949.083 kb on + strand, within ilvG at 3949.136 kb on + strand, within ilvG at 3949.136 kb on + strand, within ilvG at 3949.150 kb on + strand, within ilvG at 3949.150 kb on + strand, within ilvG at 3949.219 kb on + strand, within ilvG at 3949.265 kb on + strand, within ilvG at 3949.308 kb on - strand, within ilvG at 3949.330 kb on - strand, within ilvG at 3949.360 kb on + strand, within ilvG at 3949.360 kb on + strand, within ilvG at 3949.368 kb on - strand, within ilvG at 3949.370 kb on - strand, within ilvG at 3949.438 kb on - strand, within ilvG at 3949.443 kb on + strand, within ilvG at 3949.443 kb on + strand, within ilvG at 3949.443 kb on + strand, within ilvG at 3949.443 kb on + strand, within ilvG
Per-strain Table
Position Strand Gene LocusTag Fraction protonated glucose in D2O remove 3,947,409 - yifB b3765 0.62 -0.3 3,947,423 - yifB b3765 0.63 -0.5 3,947,423 - yifB b3765 0.63 -0.1 3,947,423 - yifB b3765 0.63 -0.3 3,947,429 + yifB b3765 0.63 -0.1 3,947,429 + yifB b3765 0.63 +0.4 3,947,437 - yifB b3765 0.63 -0.2 3,947,449 - yifB b3765 0.64 -0.4 3,947,449 - yifB b3765 0.64 -0.5 3,947,479 - yifB b3765 0.66 -0.9 3,947,481 + yifB b3765 0.66 +0.1 3,947,506 - yifB b3765 0.68 -0.3 3,947,506 - yifB b3765 0.68 -0.2 3,947,523 + yifB b3765 0.69 +0.0 3,947,523 + yifB b3765 0.69 +0.4 3,947,532 - yifB b3765 0.70 -0.2 3,947,543 + yifB b3765 0.70 +2.4 3,947,543 + yifB b3765 0.70 +0.5 3,947,746 + yifB b3765 0.84 -0.7 3,947,833 - yifB b3765 0.89 -0.3 3,947,836 - yifB b3765 0.90 -0.4 3,947,841 + +0.1 3,947,903 + +1.2 3,947,903 + +0.1 3,947,984 - +1.3 3,948,303 + -0.6 3,948,305 + -1.2 3,948,364 + ilvL b3766 0.19 +2.0 3,948,375 + ilvL b3766 0.30 -1.6 3,948,383 - ilvL b3766 0.38 -2.2 3,948,410 + ilvL b3766 0.66 -0.8 3,948,410 + ilvL b3766 0.66 -0.6 3,948,410 + ilvL b3766 0.66 +2.0 3,948,531 - -1.5 3,948,579 + -1.4 3,948,580 + -1.2 3,948,580 + +0.0 3,948,604 + -1.7 3,948,604 + -1.0 3,948,620 + -0.6 3,948,620 + -0.6 3,948,624 + -0.2 3,948,633 - +1.0 3,948,633 - -2.0 3,948,633 - -1.2 3,948,682 + -3.6 3,948,721 + +0.6 3,948,726 + -0.9 3,948,748 + ilvG b4488 0.10 -1.7 3,948,748 + ilvG b4488 0.10 -2.0 3,948,781 + ilvG b4488 0.12 -0.9 3,948,815 + ilvG b4488 0.14 -0.6 3,948,815 + ilvG b4488 0.14 -1.4 3,948,851 + ilvG b4488 0.16 -0.5 3,948,851 + ilvG b4488 0.16 -1.2 3,948,851 + ilvG b4488 0.16 -0.6 3,948,859 - ilvG b4488 0.17 -2.8 3,948,933 + ilvG b4488 0.21 -0.1 3,948,939 + ilvG b4488 0.22 -0.2 3,948,944 + ilvG b4488 0.22 -0.1 3,948,947 - ilvG b4488 0.22 -2.2 3,948,947 - ilvG b4488 0.22 -1.8 3,948,966 - ilvG b4488 0.23 -0.7 3,949,037 - ilvG b4488 0.28 -1.4 3,949,042 - ilvG b4488 0.28 -2.2 3,949,083 + ilvG b4488 0.30 +2.0 3,949,083 + ilvG b4488 0.30 +0.5 3,949,136 + ilvG b4488 0.34 -1.7 3,949,136 + ilvG b4488 0.34 -1.2 3,949,150 + ilvG b4488 0.34 +0.1 3,949,150 + ilvG b4488 0.34 -0.5 3,949,219 + ilvG b4488 0.39 +0.6 3,949,265 + ilvG b4488 0.41 +0.6 3,949,308 - ilvG b4488 0.44 -1.9 3,949,330 - ilvG b4488 0.45 -2.8 3,949,360 + ilvG b4488 0.47 +0.1 3,949,360 + ilvG b4488 0.47 -0.5 3,949,368 - ilvG b4488 0.48 -1.5 3,949,370 - ilvG b4488 0.48 -3.0 3,949,438 - ilvG b4488 0.52 -1.8 3,949,443 + ilvG b4488 0.52 -1.0 3,949,443 + ilvG b4488 0.52 -1.3 3,949,443 + ilvG b4488 0.52 -0.2 3,949,443 + ilvG b4488 0.52 +1.4
Or see this region's nucleotide sequence