Strain Fitness in Escherichia coli BW25113 around b2302

Experiment: protonated glucose in D2O

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	protonated glucose in D2O
2,417,730	+	yfcE	b2300	0.85	-0.4
2,417,745	+	yfcE	b2300	0.88	+0.1
2,417,745	+	yfcE	b2300	0.88	-1.3
2,417,753	-	yfcE	b2300	0.90	+0.0
2,417,753	-	yfcE	b2300	0.90	+0.7
2,417,759	-				+0.1
2,417,965	+	yfcF	b2301	0.16	-1.4
2,417,965	+	yfcF	b2301	0.16	-0.4
2,417,970	-	yfcF	b2301	0.17	-0.1
2,417,970	-	yfcF	b2301	0.17	-0.2
2,418,048	-	yfcF	b2301	0.29	+0.3
2,418,133	+	yfcF	b2301	0.42	-0.2
2,418,153	+	yfcF	b2301	0.45	+0.2
2,418,184	-	yfcF	b2301	0.50	+0.1
2,418,247	+	yfcF	b2301	0.60	-0.1
2,418,247	+	yfcF	b2301	0.60	+0.9
2,418,269	+	yfcF	b2301	0.63	-0.8
2,418,269	+	yfcF	b2301	0.63	-1.6
2,418,445	-				+0.5
2,418,572	-				+0.5
2,418,607	-				+0.4
2,418,607	-				+0.4
2,418,607	-				+0.7
2,418,607	-				+1.1
2,418,714	+	yfcG	b2302	0.11	-0.3
2,418,736	+	yfcG	b2302	0.14	+0.9
2,418,739	-	yfcG	b2302	0.15	+2.5
2,418,739	-	yfcG	b2302	0.15	+0.5
2,418,744	-	yfcG	b2302	0.16	+0.2
2,418,876	-	yfcG	b2302	0.36	-0.1
2,418,934	-	yfcG	b2302	0.45	+0.3
2,418,939	-	yfcG	b2302	0.46	-1.0
2,418,970	-	yfcG	b2302	0.50	+0.3
2,419,017	-	yfcG	b2302	0.58	+0.3
2,419,052	-	yfcG	b2302	0.63	-0.0
2,419,064	+	yfcG	b2302	0.65	+0.7
2,419,077	-	yfcG	b2302	0.67	+0.8
2,419,077	-	yfcG	b2302	0.67	-0.1
2,419,108	-	yfcG	b2302	0.72	+0.1
2,419,109	-	yfcG	b2302	0.72	+0.2
2,419,186	+	yfcG	b2302	0.84	+0.8
2,419,188	+	yfcG	b2302	0.84	+0.5
2,419,213	+	yfcG	b2302	0.88	-0.4
2,419,213	+	yfcG	b2302	0.88	+0.7
2,419,222	+	yfcG	b2302	0.89	+0.0
2,419,223	-	yfcG	b2302	0.90	+0.1
2,419,241	+				+1.0
2,419,251	+				-0.7
2,419,267	+				+0.5
2,419,349	+				+1.2
2,419,398	-	folX	b2303	0.14	-0.3
2,419,398	-	folX	b2303	0.14	+0.2
2,419,452	+	folX	b2303	0.29	+0.3
2,419,452	+	folX	b2303	0.29	+0.3
2,419,458	+	folX	b2303	0.31	-0.2
2,419,458	+	folX	b2303	0.31	-1.0
2,419,458	+	folX	b2303	0.31	-0.9
2,419,458	+	folX	b2303	0.31	-0.4
2,419,458	-	folX	b2303	0.31	-1.1
2,419,458	-	folX	b2303	0.31	-0.3
2,419,460	-	folX	b2303	0.31	+0.7
2,419,460	-	folX	b2303	0.31	+0.1
2,419,466	-	folX	b2303	0.33	-1.5
2,419,466	-	folX	b2303	0.33	-0.0
2,419,468	-	folX	b2303	0.33	-0.1
2,419,468	-	folX	b2303	0.33	+0.4
2,419,471	+	folX	b2303	0.34	-0.6
2,419,471	+	folX	b2303	0.34	-0.5
2,419,478	+	folX	b2303	0.36	+0.0
2,419,494	+	folX	b2303	0.40	-0.6
2,419,593	+	folX	b2303	0.68	+0.8
2,419,603	-	folX	b2303	0.71	+0.4
2,419,614	-	folX	b2303	0.74	+0.5
2,419,619	+	folX	b2303	0.75	+0.7
2,419,624	-	folX	b2303	0.76	+0.7
2,419,630	+	folX	b2303	0.78	+0.2
2,419,647	+	folX	b2303	0.83	-0.7
2,419,647	+	folX	b2303	0.83	-0.2
2,419,650	+	folX	b2303	0.83	-0.5
2,419,653	-	folX	b2303	0.84	+0.2
2,419,683	+				-0.8
2,419,689	+				+0.2
2,419,689	+				+0.9
2,419,697	-				-0.3
2,419,720	-				+0.6
2,419,720	-				+0.2
2,419,725	-				+0.7
2,419,726	+				+0.2
2,419,728	+				+0.4
2,419,736	-				+0.3
2,419,736	-				+1.3
2,419,809	+				+0.3
2,419,816	+				-0.6
2,419,816	+				-0.1
2,419,821	+	yfcH	b2304	0.10	-0.2
2,419,821	+	yfcH	b2304	0.10	+1.3
2,419,865	+	yfcH	b2304	0.15	+0.5
2,419,874	-	yfcH	b2304	0.16	-0.2
2,420,059	-	yfcH	b2304	0.37	+1.5
2,420,077	-	yfcH	b2304	0.39	-0.5
2,420,077	-	yfcH	b2304	0.39	+0.5
2,420,089	-	yfcH	b2304	0.40	+0.2
2,420,225	-	yfcH	b2304	0.55	+0.7
2,420,225	-	yfcH	b2304	0.55	+0.4
2,420,228	-	yfcH	b2304	0.56	-0.4
2,420,228	-	yfcH	b2304	0.56	+0.6
2,420,267	+	yfcH	b2304	0.60	-0.2
2,420,267	+	yfcH	b2304	0.60	-0.5