Strain Fitness in Escherichia coli BW25113 around b2026

Experiment: protonated glucose in D2O

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisA and hisF overlap by 19 nucleotideshisF and hisI overlap by 7 nucleotideshisI and wzzB are separated by 95 nucleotides b2024: hisA - N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS), at 2,093,149 to 2,093,886 hisA b2025: hisF - imidazole glycerol phosphate synthase subunit HisF (NCBI), at 2,093,868 to 2,094,644 hisF b2026: hisI - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (NCBI), at 2,094,638 to 2,095,249 hisI b2027: wzzB - regulator of length of O-antigen component of lipopolysaccharide chains (VIMSS), at 2,095,345 to 2,096,325 wzzB Position (kb) 2094 2095 2096Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 2093.818 kb on - strandat 2093.818 kb on - strandat 2093.881 kb on + strandat 2093.918 kb on + strandat 2093.978 kb on + strand, within hisFat 2094.236 kb on + strand, within hisFat 2094.236 kb on + strand, within hisFat 2094.236 kb on + strand, within hisFat 2094.244 kb on - strand, within hisFat 2094.244 kb on - strand, within hisFat 2094.248 kb on - strand, within hisFat 2094.338 kb on - strand, within hisFat 2094.596 kb on + strandat 2094.596 kb on + strandat 2094.647 kb on + strandat 2094.712 kb on - strand, within hisIat 2094.712 kb on - strand, within hisIat 2094.716 kb on + strand, within hisIat 2094.869 kb on + strand, within hisIat 2094.907 kb on - strand, within hisIat 2094.907 kb on - strand, within hisIat 2095.032 kb on + strand, within hisIat 2095.033 kb on + strand, within hisIat 2095.033 kb on + strand, within hisIat 2095.124 kb on + strand, within hisIat 2095.167 kb on + strand, within hisIat 2095.202 kb on - strandat 2095.231 kb on + strandat 2095.386 kb on - strandat 2095.490 kb on + strand, within wzzBat 2095.494 kb on + strand, within wzzBat 2095.586 kb on - strand, within wzzBat 2095.586 kb on - strand, within wzzBat 2095.606 kb on - strand, within wzzBat 2095.719 kb on - strand, within wzzBat 2095.719 kb on - strand, within wzzBat 2095.729 kb on - strand, within wzzBat 2095.819 kb on + strand, within wzzBat 2095.819 kb on + strand, within wzzBat 2095.900 kb on + strand, within wzzBat 2095.900 kb on + strand, within wzzBat 2096.075 kb on + strand, within wzzBat 2096.075 kb on + strand, within wzzBat 2096.102 kb on + strand, within wzzBat 2096.163 kb on - strand, within wzzB

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Per-strain Table

Position Strand Gene LocusTag Fraction protonated glucose in D2O
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2,093,818 - -3.7
2,093,818 - -3.7
2,093,881 + -2.9
2,093,918 + -4.9
2,093,978 + hisF b2025 0.14 -1.4
2,094,236 + hisF b2025 0.47 -3.4
2,094,236 + hisF b2025 0.47 -3.1
2,094,236 + hisF b2025 0.47 -5.0
2,094,244 - hisF b2025 0.48 -0.2
2,094,244 - hisF b2025 0.48 -4.6
2,094,248 - hisF b2025 0.49 -2.1
2,094,338 - hisF b2025 0.60 -2.7
2,094,596 + -1.3
2,094,596 + -1.9
2,094,647 + +1.8
2,094,712 - hisI b2026 0.12 -2.5
2,094,712 - hisI b2026 0.12 -2.9
2,094,716 + hisI b2026 0.13 -5.0
2,094,869 + hisI b2026 0.38 -3.0
2,094,907 - hisI b2026 0.44 -3.7
2,094,907 - hisI b2026 0.44 -0.6
2,095,032 + hisI b2026 0.64 -4.4
2,095,033 + hisI b2026 0.65 -2.6
2,095,033 + hisI b2026 0.65 -4.1
2,095,124 + hisI b2026 0.79 -4.6
2,095,167 + hisI b2026 0.86 -4.8
2,095,202 - -3.2
2,095,231 + -0.3
2,095,386 - +0.5
2,095,490 + wzzB b2027 0.15 -0.2
2,095,494 + wzzB b2027 0.15 -0.4
2,095,586 - wzzB b2027 0.25 +0.0
2,095,586 - wzzB b2027 0.25 +0.5
2,095,606 - wzzB b2027 0.27 +0.1
2,095,719 - wzzB b2027 0.38 +0.8
2,095,719 - wzzB b2027 0.38 -0.4
2,095,729 - wzzB b2027 0.39 +0.3
2,095,819 + wzzB b2027 0.48 +0.5
2,095,819 + wzzB b2027 0.48 +0.5
2,095,900 + wzzB b2027 0.57 +1.1
2,095,900 + wzzB b2027 0.57 +0.6
2,096,075 + wzzB b2027 0.74 +0.2
2,096,075 + wzzB b2027 0.74 +2.1
2,096,102 + wzzB b2027 0.77 -0.1
2,096,163 - wzzB b2027 0.83 +0.4

Or see this region's nucleotide sequence