Experiment: protonated glucose in D2O
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybdK and sokE are separated by 350 nucleotides sokE and hokE are separated by 43 nucleotides hokE and insL-2 are separated by 76 nucleotides insL-2 and entD are separated by 281 nucleotides
b0581: ybdK - gamma-glutamyl:cysteine ligase (NCBI), at 605,488 to 606,606
ybdK
b4700: sokE - sRNA at remnant mokE/hokE locus (RefSeq), at 606,957 to 607,015
sokE
b4415: hokE - toxic polypeptide, small (NCBI), at 607,059 to 607,211
hokE
b0582: insL-2 - IS186/IS421 transposase (NCBI), at 607,288 to 608,400
insL-2
b0583: entD - phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex (NCBI), at 608,682 to 609,302
entD
Position (kb) 607
608
609 Strain fitness (log2 ratio) -1
0
1 at 606.317 kb on - strand, within ybdK at 606.367 kb on - strand, within ybdK at 606.498 kb on + strand at 606.506 kb on - strand at 606.516 kb on - strand at 606.516 kb on - strand at 606.602 kb on + strand at 606.621 kb on - strand at 606.673 kb on + strand at 606.937 kb on + strand at 606.988 kb on + strand, within sokE at 607.102 kb on - strand, within hokE at 607.105 kb on - strand, within hokE at 607.105 kb on - strand, within hokE at 607.122 kb on + strand, within hokE at 607.185 kb on + strand, within hokE at 607.210 kb on - strand at 607.224 kb on + strand at 608.621 kb on + strand at 608.621 kb on + strand at 608.621 kb on + strand at 608.629 kb on - strand at 608.669 kb on - strand at 608.750 kb on - strand, within entD at 608.761 kb on + strand, within entD at 608.766 kb on + strand, within entD at 608.834 kb on + strand, within entD at 609.183 kb on + strand, within entD at 609.246 kb on - strand at 609.246 kb on - strand at 609.253 kb on + strand at 609.253 kb on + strand at 609.266 kb on + strand at 609.266 kb on + strand at 609.277 kb on - strand at 609.277 kb on - strand at 609.284 kb on - strand at 609.303 kb on - strand at 609.304 kb on - strand at 609.338 kb on - strand at 609.338 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction protonated glucose in D2O remove 606,317 - ybdK b0581 0.74 +0.8 606,367 - ybdK b0581 0.79 -0.5 606,498 + +0.1 606,506 - +1.0 606,516 - +0.4 606,516 - +0.2 606,602 + +1.6 606,621 - +0.6 606,673 + +0.2 606,937 + +0.7 606,988 + sokE b4700 0.53 -1.2 607,102 - hokE b4415 0.28 +0.9 607,105 - hokE b4415 0.30 -0.1 607,105 - hokE b4415 0.30 -0.5 607,122 + hokE b4415 0.41 -0.5 607,185 + hokE b4415 0.82 +0.0 607,210 - -0.4 607,224 + +1.4 608,621 + -0.2 608,621 + +1.1 608,621 + -0.4 608,629 - +0.7 608,669 - -0.4 608,750 - entD b0583 0.11 +0.3 608,761 + entD b0583 0.13 -0.3 608,766 + entD b0583 0.14 -0.7 608,834 + entD b0583 0.24 -0.2 609,183 + entD b0583 0.81 -1.1 609,246 - +0.7 609,246 - -0.8 609,253 + +0.8 609,253 + +1.2 609,266 + -0.9 609,266 + -0.3 609,277 - +1.6 609,277 - +0.1 609,284 - +0.0 609,303 - +1.1 609,304 - +0.2 609,338 - +0.7 609,338 - -0.1
Or see this region's nucleotide sequence