Strain Fitness in Escherichia coli BW25113 around b3454

Experiment: protonated control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntugpB and livF are separated by 398 nucleotideslivF and livG are separated by 1 nucleotideslivG and livM overlap by 4 nucleotides b3453: ugpB - glycerol-3-phosphate transporter subunit (NCBI), at 3,589,032 to 3,590,348 ugpB b3454: livF - ATP-binding component of leucine transport (VIMSS), at 3,590,747 to 3,591,460 livF b3455: livG - leucine/isoleucine/valine transporter subunit (NCBI), at 3,591,462 to 3,592,229 livG b3456: livM - leucine/isoleucine/valine transporter subunit (NCBI), at 3,592,226 to 3,593,503 livM Position (kb) 3590 3591 3592Strain fitness (log2 ratio) -1 0 1 2 3at 3589.777 kb on + strand, within ugpBat 3589.777 kb on + strand, within ugpBat 3589.998 kb on - strand, within ugpBat 3589.998 kb on - strand, within ugpBat 3590.230 kb on + strandat 3590.271 kb on + strandat 3590.305 kb on + strandat 3590.319 kb on + strandat 3590.390 kb on + strandat 3590.403 kb on + strandat 3590.411 kb on - strandat 3590.450 kb on + strandat 3590.459 kb on + strandat 3590.655 kb on + strandat 3590.659 kb on - strandat 3590.659 kb on - strandat 3590.703 kb on - strandat 3590.774 kb on - strandat 3590.810 kb on - strandat 3590.898 kb on - strand, within livFat 3590.898 kb on - strand, within livFat 3590.903 kb on - strand, within livFat 3590.966 kb on - strand, within livFat 3590.966 kb on - strand, within livFat 3590.988 kb on + strand, within livFat 3591.059 kb on - strand, within livFat 3591.061 kb on + strand, within livFat 3591.090 kb on - strand, within livFat 3591.125 kb on - strand, within livFat 3591.164 kb on + strand, within livFat 3591.164 kb on + strand, within livFat 3591.172 kb on - strand, within livFat 3591.197 kb on + strand, within livFat 3591.252 kb on + strand, within livFat 3591.252 kb on + strand, within livFat 3591.381 kb on - strand, within livFat 3591.381 kb on - strand, within livFat 3591.419 kb on - strandat 3591.479 kb on - strandat 3591.479 kb on - strandat 3591.547 kb on - strand, within livGat 3591.584 kb on + strand, within livGat 3591.592 kb on - strand, within livGat 3591.748 kb on - strand, within livGat 3591.748 kb on - strand, within livGat 3591.748 kb on - strand, within livGat 3591.765 kb on + strand, within livGat 3591.768 kb on - strand, within livGat 3591.834 kb on + strand, within livGat 3591.834 kb on + strand, within livGat 3591.881 kb on + strand, within livGat 3591.907 kb on + strand, within livGat 3591.937 kb on - strand, within livGat 3591.943 kb on - strand, within livGat 3591.962 kb on - strand, within livGat 3591.974 kb on - strand, within livGat 3592.053 kb on + strand, within livGat 3592.148 kb on - strand, within livGat 3592.229 kb on + strandat 3592.321 kb on - strandat 3592.436 kb on + strand, within livMat 3592.436 kb on + strand, within livM

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Per-strain Table

Position Strand Gene LocusTag Fraction protonated control
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3,589,777 + ugpB b3453 0.57 -0.8
3,589,777 + ugpB b3453 0.57 +0.5
3,589,998 - ugpB b3453 0.73 -0.3
3,589,998 - ugpB b3453 0.73 +0.0
3,590,230 + -0.2
3,590,271 + -0.9
3,590,305 + -0.1
3,590,319 + -0.2
3,590,390 + -0.8
3,590,403 + +1.8
3,590,411 - +1.6
3,590,450 + -0.3
3,590,459 + +1.8
3,590,655 + -0.2
3,590,659 - -0.1
3,590,659 - +0.9
3,590,703 - -0.2
3,590,774 - +1.5
3,590,810 - +1.7
3,590,898 - livF b3454 0.21 +0.1
3,590,898 - livF b3454 0.21 -0.3
3,590,903 - livF b3454 0.22 +0.8
3,590,966 - livF b3454 0.31 +0.9
3,590,966 - livF b3454 0.31 +0.3
3,590,988 + livF b3454 0.34 +1.5
3,591,059 - livF b3454 0.44 +1.6
3,591,061 + livF b3454 0.44 +1.4
3,591,090 - livF b3454 0.48 +0.9
3,591,125 - livF b3454 0.53 +1.8
3,591,164 + livF b3454 0.58 +1.3
3,591,164 + livF b3454 0.58 +0.6
3,591,172 - livF b3454 0.60 +1.4
3,591,197 + livF b3454 0.63 +1.7
3,591,252 + livF b3454 0.71 +1.6
3,591,252 + livF b3454 0.71 +1.0
3,591,381 - livF b3454 0.89 +0.5
3,591,381 - livF b3454 0.89 -0.0
3,591,419 - -0.1
3,591,479 - +1.8
3,591,479 - -0.7
3,591,547 - livG b3455 0.11 +0.5
3,591,584 + livG b3455 0.16 +1.8
3,591,592 - livG b3455 0.17 +0.5
3,591,748 - livG b3455 0.37 +1.3
3,591,748 - livG b3455 0.37 +2.8
3,591,748 - livG b3455 0.37 +0.7
3,591,765 + livG b3455 0.39 +0.4
3,591,768 - livG b3455 0.40 +1.6
3,591,834 + livG b3455 0.48 +2.4
3,591,834 + livG b3455 0.48 +0.7
3,591,881 + livG b3455 0.55 +1.6
3,591,907 + livG b3455 0.58 -1.2
3,591,937 - livG b3455 0.62 +0.9
3,591,943 - livG b3455 0.63 +0.6
3,591,962 - livG b3455 0.65 +0.8
3,591,974 - livG b3455 0.67 -0.0
3,592,053 + livG b3455 0.77 +0.9
3,592,148 - livG b3455 0.89 +1.4
3,592,229 + +0.5
3,592,321 - +1.0
3,592,436 + livM b3456 0.16 +1.3
3,592,436 + livM b3456 0.16 +1.4

Or see this region's nucleotide sequence