Experiment: Beta-Lactose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0357 and Echvi_0358 are separated by 387 nucleotides Echvi_0358 and Echvi_0359 overlap by 8 nucleotides Echvi_0359 and Echvi_0360 are separated by 24 nucleotides
Echvi_0357: Echvi_0357 - hypothetical protein, at 385,126 to 385,608
_0357
Echvi_0358: Echvi_0358 - RNA polymerase sigma factor, sigma-70 family, at 385,996 to 386,565
_0358
Echvi_0359: Echvi_0359 - hypothetical protein, at 386,558 to 387,343
_0359
Echvi_0360: Echvi_0360 - hypothetical protein, at 387,368 to 388,405
_0360
Position (kb) 386
387
388 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 385.592 kb on - strand at 385.603 kb on + strand at 385.614 kb on + strand at 385.627 kb on - strand at 385.657 kb on + strand at 385.660 kb on + strand at 385.660 kb on + strand at 385.660 kb on + strand at 385.661 kb on - strand at 385.661 kb on - strand at 385.688 kb on - strand at 385.691 kb on - strand at 385.724 kb on + strand at 385.768 kb on - strand at 385.831 kb on + strand at 385.832 kb on - strand at 385.900 kb on + strand at 385.900 kb on + strand at 385.900 kb on + strand at 385.901 kb on - strand at 385.901 kb on - strand at 385.904 kb on + strand at 385.904 kb on + strand at 385.904 kb on + strand at 385.905 kb on - strand at 385.909 kb on + strand at 385.910 kb on - strand at 386.036 kb on + strand at 386.036 kb on + strand at 386.037 kb on - strand at 386.080 kb on + strand, within Echvi_0358 at 386.081 kb on - strand, within Echvi_0358 at 386.081 kb on - strand, within Echvi_0358 at 386.111 kb on + strand, within Echvi_0358 at 386.111 kb on + strand, within Echvi_0358 at 386.111 kb on + strand, within Echvi_0358 at 386.123 kb on - strand, within Echvi_0358 at 386.123 kb on - strand, within Echvi_0358 at 386.125 kb on - strand, within Echvi_0358 at 386.197 kb on + strand, within Echvi_0358 at 386.294 kb on + strand, within Echvi_0358 at 386.333 kb on - strand, within Echvi_0358 at 386.417 kb on - strand, within Echvi_0358 at 386.417 kb on - strand, within Echvi_0358 at 386.417 kb on - strand, within Echvi_0358 at 386.432 kb on - strand, within Echvi_0358 at 386.513 kb on + strand at 386.514 kb on - strand at 386.514 kb on - strand at 386.535 kb on - strand at 386.535 kb on - strand at 386.691 kb on + strand, within Echvi_0359 at 386.691 kb on + strand, within Echvi_0359 at 386.734 kb on + strand, within Echvi_0359 at 387.264 kb on + strand, within Echvi_0359 at 387.608 kb on - strand, within Echvi_0360 at 388.193 kb on + strand, within Echvi_0360
Per-strain Table
Position Strand Gene LocusTag Fraction Beta-Lactose (C) remove 385,592 - +0.6 385,603 + +0.8 385,614 + -0.8 385,627 - -0.8 385,657 + -0.0 385,660 + -2.0 385,660 + -3.0 385,660 + -0.7 385,661 - -0.3 385,661 - +0.2 385,688 - +0.8 385,691 - -0.7 385,724 + +0.0 385,768 - -0.3 385,831 + -2.3 385,832 - -1.0 385,900 + -0.6 385,900 + -0.2 385,900 + -0.9 385,901 - -1.1 385,901 - -0.3 385,904 + -0.3 385,904 + +0.8 385,904 + +1.9 385,905 - -0.3 385,909 + +1.6 385,910 - -0.3 386,036 + +0.9 386,036 + +0.8 386,037 - -0.4 386,080 + Echvi_0358 0.15 +0.6 386,081 - Echvi_0358 0.15 -0.1 386,081 - Echvi_0358 0.15 +0.5 386,111 + Echvi_0358 0.20 +1.7 386,111 + Echvi_0358 0.20 +1.5 386,111 + Echvi_0358 0.20 -1.0 386,123 - Echvi_0358 0.22 -0.6 386,123 - Echvi_0358 0.22 -0.5 386,125 - Echvi_0358 0.23 +0.1 386,197 + Echvi_0358 0.35 -0.3 386,294 + Echvi_0358 0.52 -0.4 386,333 - Echvi_0358 0.59 -1.5 386,417 - Echvi_0358 0.74 -1.0 386,417 - Echvi_0358 0.74 -0.3 386,417 - Echvi_0358 0.74 -0.7 386,432 - Echvi_0358 0.76 -0.2 386,513 + -0.4 386,514 - -1.5 386,514 - -0.6 386,535 - -0.4 386,535 - +1.2 386,691 + Echvi_0359 0.17 -0.5 386,691 + Echvi_0359 0.17 -2.6 386,734 + Echvi_0359 0.22 +0.2 387,264 + Echvi_0359 0.90 -1.7 387,608 - Echvi_0360 0.23 +0.8 388,193 + Echvi_0360 0.79 +0.4
Or see this region's nucleotide sequence