Experiment: R2A outgrowth control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS25520 and ABID97_RS25525 are separated by 207 nucleotides ABID97_RS25525 and ABID97_RS25530 are separated by 387 nucleotides
ABID97_RS25520: ABID97_RS25520 - branched-chain amino acid ABC transporter permease, at 21,502 to 22,419
_RS25520
ABID97_RS25525: ABID97_RS25525 - LacI family DNA-binding transcriptional regulator, at 22,627 to 23,676
_RS25525
ABID97_RS25530: ABID97_RS25530 - cyclase family protein, at 24,064 to 25,026
_RS25530
Position (kb) 22
23
24 Strain fitness (log2 ratio) -1
0
1 at 22.287 kb on - strand, within ABID97_RS25520 at 22.554 kb on + strand at 22.568 kb on + strand at 22.576 kb on - strand at 22.577 kb on + strand at 22.577 kb on + strand at 22.578 kb on - strand at 22.628 kb on + strand at 23.482 kb on - strand, within ABID97_RS25525 at 23.522 kb on + strand, within ABID97_RS25525 at 23.744 kb on - strand at 23.904 kb on + strand at 24.164 kb on - strand, within ABID97_RS25530 at 24.316 kb on + strand, within ABID97_RS25530 at 24.352 kb on + strand, within ABID97_RS25530 at 24.352 kb on + strand, within ABID97_RS25530 at 24.382 kb on + strand, within ABID97_RS25530 at 24.403 kb on + strand, within ABID97_RS25530 at 24.452 kb on - strand, within ABID97_RS25530
Per-strain Table
Position Strand Gene LocusTag Fraction R2A outgrowth control remove 22,287 - ABID97_RS25520 0.86 +1.6 22,554 + -0.0 22,568 + -0.1 22,576 - +0.1 22,577 + +0.0 22,577 + -0.3 22,578 - -0.1 22,628 + -0.4 23,482 - ABID97_RS25525 0.81 -0.9 23,522 + ABID97_RS25525 0.85 -0.7 23,744 - -0.1 23,904 + +0.2 24,164 - ABID97_RS25530 0.10 -0.0 24,316 + ABID97_RS25530 0.26 +0.4 24,352 + ABID97_RS25530 0.30 -0.1 24,352 + ABID97_RS25530 0.30 +0.3 24,382 + ABID97_RS25530 0.33 -0.0 24,403 + ABID97_RS25530 0.35 -0.1 24,452 - ABID97_RS25530 0.40 +0.0
Or see this region's nucleotide sequence